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The map label for this gene is pleC [H]

Identifier: 209548975

GI number: 209548975

Start: 1390652

End: 1392184

Strand: Reverse

Name: pleC [H]

Synonym: Rleg2_1372

Alternate gene names: 209548975

Gene position: 1392184-1390652 (Counterclockwise)

Preceding gene: 209548976

Following gene: 209548974

Centisome position: 30.68

GC content: 60.14

Gene sequence:

>1533_bases
ATGCCACACTCGTTTGAATCCATTCTGGAAAACTTGCCGCAGGGCATTGTTCTCCTTGATCCTGCCAATAGGGTGACGGC
TTTCAATTCCCGCACGCTTGAGATTCTCGGGCTGCCGGATGGAGCCATCGGCAAAGGAATGAATATCGACCGACTGCCGG
GCACCGCCGATTGTCGCGCGGCCGCATGCAGGCAGTCGACGACCGGCAGGCCGCACACGTCTCAGTTTGCGCTTTCCGAC
GGACGGATGGTCTCAATGACCTGCGCCCCATCGAAAGAGGGAGGCTGGATCCTCAGCTACGAAGACATCTCGACCCTGAT
CCGCGTCGAAGACAGTCTCACCGAACAACATCACCGGTTCGACGCAGCACTCAGCAACATGCCGCACGGCCTATGCATGT
TCGATTCGACGAAGAAGCTGATCCTGTGCAACCCAGCCTACGCGCGGATGTACAATCTGCCGGATCTCCTGACGCAACCG
GGGACGCCGCTTGTCGATATCCTGGCCTATCGCGGCCGCGAGGGAAATGGCCCGGCCGACACCGCTACCTATTTCGATGT
GGTGTTCGAAGCAACGGCGCGCGGCGCGGTGGCCAGCCAGAATATCGTGCTCGTTGACGGCCGCGTTATCAAAATCACCC
ACAATCCGATGCAGAACGGTGGCTACGTGGCCACCCACGAAGACGTCACCAAAACAGTGCGGCTGACCGAAGAGCTGCGC
CGGCATCACGATCAGCTCGAAACGACGGTGCAGAGCCGCACAGCCGAGGTCGAGCGGCAGGCGCGGGAACTCGAGCGAAT
GCTTGCGCAGGAGCGAAACATCAACGAATTGCAGCGGCAGTTCGTGGCGATGGCCTCCCATGAATTCCGAACGCCGCTGG
CCATCATCGACGCTGCCGCCCAGCGGCTGCTGCGCAAAAGAGGAGCAGTCGAGCCCGAATTCTTGAGCGACAAGGTCGAT
CAGATCCGCGCATCGGTGAACCGGATCGTCGACCTGATGGAGAGTATCCTTTCGGCCGGGCGCCTCGACACCGGCAAGAT
CGATATCAGCTATGACGCCTGCGCCTTGCGATCATTGATCAGAAGCTGTTGCGAGCGGCAGTCGACGATCGCCAAGACGC
ACAGGTTCGTGCTGGATATCGACGGGCTGCCGGAGTTCATCGATGCCGATCCCCGAACCGTGGCGCAGGTCTTTACCAAC
CTGCTGTCCAACGCCGTCAAATATGCGCCCGGAACCTCGGAAATTCGCGTCACGGCATGGGAGGAGACGGGAAACGTCAA
AGTTTCGATCAGCGATGACGGGGTGGGGATCGATCCTGAAGACGTGCCCCGGCTGTTCCAGCGCTATTTTCGGGCCGGGA
CGTCGACCGGAATTGCCGGGACGGGCATCGGCCTCAACCTGGTGAAGCAGATCGTCGAACTGCACCACGGCTCGATAGAG
GTTCGAAGCGCAAGAGGCTGCGGATCGACATTCACCGTAACCCTTCCGATCAAGCGAACTGGTAACGCTTCTGAGAACAG
CGACGCCGCCTGA

Upstream 100 bases:

>100_bases
AAGTGAGGAAGTCCGATCGATAGCCATTAGCGAAACTTTATCGTTTCTGACGAATGCTACGAATACGTGCAGCGTGCCAG
ATCTGAAGCAAGGCCGGATG

Downstream 100 bases:

>100_bases
CATTTCAGGATTGCTGTCCTCAATATCTACCCTATCCTTCTCGCTATAGAGGAGGACCATGCCATGGCTTTCATTCACAC
CCCCAATGCATCCGCCAGCG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MPHSFESILENLPQGIVLLDPANRVTAFNSRTLEILGLPDGAIGKGMNIDRLPGTADCRAAACRQSTTGRPHTSQFALSD
GRMVSMTCAPSKEGGWILSYEDISTLIRVEDSLTEQHHRFDAALSNMPHGLCMFDSTKKLILCNPAYARMYNLPDLLTQP
GTPLVDILAYRGREGNGPADTATYFDVVFEATARGAVASQNIVLVDGRVIKITHNPMQNGGYVATHEDVTKTVRLTEELR
RHHDQLETTVQSRTAEVERQARELERMLAQERNINELQRQFVAMASHEFRTPLAIIDAAAQRLLRKRGAVEPEFLSDKVD
QIRASVNRIVDLMESILSAGRLDTGKIDISYDACALRSLIRSCCERQSTIAKTHRFVLDIDGLPEFIDADPRTVAQVFTN
LLSNAVKYAPGTSEIRVTAWEETGNVKVSISDDGVGIDPEDVPRLFQRYFRAGTSTGIAGTGIGLNLVKQIVELHHGSIE
VRSARGCGSTFTVTLPIKRTGNASENSDAA

Sequences:

>Translated_510_residues
MPHSFESILENLPQGIVLLDPANRVTAFNSRTLEILGLPDGAIGKGMNIDRLPGTADCRAAACRQSTTGRPHTSQFALSD
GRMVSMTCAPSKEGGWILSYEDISTLIRVEDSLTEQHHRFDAALSNMPHGLCMFDSTKKLILCNPAYARMYNLPDLLTQP
GTPLVDILAYRGREGNGPADTATYFDVVFEATARGAVASQNIVLVDGRVIKITHNPMQNGGYVATHEDVTKTVRLTEELR
RHHDQLETTVQSRTAEVERQARELERMLAQERNINELQRQFVAMASHEFRTPLAIIDAAAQRLLRKRGAVEPEFLSDKVD
QIRASVNRIVDLMESILSAGRLDTGKIDISYDACALRSLIRSCCERQSTIAKTHRFVLDIDGLPEFIDADPRTVAQVFTN
LLSNAVKYAPGTSEIRVTAWEETGNVKVSISDDGVGIDPEDVPRLFQRYFRAGTSTGIAGTGIGLNLVKQIVELHHGSIE
VRSARGCGSTFTVTLPIKRTGNASENSDAA
>Mature_509_residues
PHSFESILENLPQGIVLLDPANRVTAFNSRTLEILGLPDGAIGKGMNIDRLPGTADCRAAACRQSTTGRPHTSQFALSDG
RMVSMTCAPSKEGGWILSYEDISTLIRVEDSLTEQHHRFDAALSNMPHGLCMFDSTKKLILCNPAYARMYNLPDLLTQPG
TPLVDILAYRGREGNGPADTATYFDVVFEATARGAVASQNIVLVDGRVIKITHNPMQNGGYVATHEDVTKTVRLTEELRR
HHDQLETTVQSRTAEVERQARELERMLAQERNINELQRQFVAMASHEFRTPLAIIDAAAQRLLRKRGAVEPEFLSDKVDQ
IRASVNRIVDLMESILSAGRLDTGKIDISYDACALRSLIRSCCERQSTIAKTHRFVLDIDGLPEFIDADPRTVAQVFTNL
LSNAVKYAPGTSEIRVTAWEETGNVKVSISDDGVGIDPEDVPRLFQRYFRAGTSTGIAGTGIGLNLVKQIVELHHGSIEV
RSARGCGSTFTVTLPIKRTGNASENSDAA

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=29.8387096774194, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=28.0172413793103, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081816, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786600, Length=281, Percent_Identity=26.3345195729537, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=27.9835390946502, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790861, Length=235, Percent_Identity=25.9574468085106, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=28.7878787878788, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=253, Percent_Identity=26.4822134387352, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=26.2096774193548, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1786783, Length=250, Percent_Identity=28, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=246, Percent_Identity=24.390243902439, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788279, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=26.5765765765766, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790551, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790346, Length=266, Percent_Identity=21.4285714285714, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789403, Length=240, Percent_Identity=25.8333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787894, Length=233, Percent_Identity=26.6094420600858, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55999; Mature: 55868

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSFESILENLPQGIVLLDPANRVTAFNSRTLEILGLPDGAIGKGMNIDRLPGTADCRA
CCCCHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
AACRQSTTGRPHTSQFALSDGRMVSMTCAPSKEGGWILSYEDISTLIRVEDSLTEQHHRF
HHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
DAALSNMPHGLCMFDSTKKLILCNPAYARMYNLPDLLTQPGTPLVDILAYRGREGNGPAD
HHHHHCCCCCEEEECCCCEEEEECCHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCC
TATYFDVVFEATARGAVASQNIVLVDGRVIKITHNPMQNGGYVATHEDVTKTVRLTEELR
HHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
RHHDQLETTVQSRTAEVERQARELERMLAQERNINELQRQFVAMASHEFRTPLAIIDAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
QRLLRKRGAVEPEFLSDKVDQIRASVNRIVDLMESILSAGRLDTGKIDISYDACALRSLI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHH
RSCCERQSTIAKTHRFVLDIDGLPEFIDADPRTVAQVFTNLLSNAVKYAPGTSEIRVTAW
HHHHHHHHHHHHHHEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EETGNVKVSISDDGVGIDPEDVPRLFQRYFRAGTSTGIAGTGIGLNLVKQIVELHHGSIE
ECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VRSARGCGSTFTVTLPIKRTGNASENSDAA
EEECCCCCCEEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PHSFESILENLPQGIVLLDPANRVTAFNSRTLEILGLPDGAIGKGMNIDRLPGTADCRA
CCCHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
AACRQSTTGRPHTSQFALSDGRMVSMTCAPSKEGGWILSYEDISTLIRVEDSLTEQHHRF
HHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
DAALSNMPHGLCMFDSTKKLILCNPAYARMYNLPDLLTQPGTPLVDILAYRGREGNGPAD
HHHHHCCCCCEEEECCCCEEEEECCHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCC
TATYFDVVFEATARGAVASQNIVLVDGRVIKITHNPMQNGGYVATHEDVTKTVRLTEELR
HHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
RHHDQLETTVQSRTAEVERQARELERMLAQERNINELQRQFVAMASHEFRTPLAIIDAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
QRLLRKRGAVEPEFLSDKVDQIRASVNRIVDLMESILSAGRLDTGKIDISYDACALRSLI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHH
RSCCERQSTIAKTHRFVLDIDGLPEFIDADPRTVAQVFTNLLSNAVKYAPGTSEIRVTAW
HHHHHHHHHHHHHHEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EETGNVKVSISDDGVGIDPEDVPRLFQRYFRAGTSTGIAGTGIGLNLVKQIVELHHGSIE
ECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VRSARGCGSTFTVTLPIKRTGNASENSDAA
EEECCCCCCEEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]