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The map label for this gene is nadE [H]

Identifier: 209548440

GI number: 209548440

Start: 857204

End: 858037

Strand: Direct

Name: nadE [H]

Synonym: Rleg2_0837

Alternate gene names: 209548440

Gene position: 857204-858037 (Clockwise)

Preceding gene: 209548439

Following gene: 209548442

Centisome position: 18.89

GC content: 66.55

Gene sequence:

>834_bases
ATGACCGTGCTGTCCGATGAACAAGGTGAGATCATCCGTGAGCTGGGCGTCGCCGCCGATATCGATCCCGAGCGTGAGAT
CGAGCGGCGAACCGAATTCCTCAAGGATTATCTCGTGGCCTCGGGCATGCGCGGCTATGTCCTCGGCATCAGCGGCGGCG
TCGATTCGCTGACGGCGGCGCTGATTGCCCAGAAGGCGGTGCGCGAGCTGCGCGAAAGCGGCCATGCGGCGGAGTTCATT
GCCGTGCGTCTTCCCTATGACGTCCAGGCGGATGAGGCCGATGCCGAGCGGGCGCTGGCGACAATCGGCGCCGACCGCGC
GATGGTGGTCAACATCAAGGCGGCGGCGGATGCGATGCTGGCGGCCGCGCAAAACGGCGGTCTTGCCTTTGCCGATGCCG
GCCGGCAGGATTTCATCCTCGGCAACATCAAGGCCCGCCAGCGCATGATCGCCCAGTTCGCTCTTGCCGGCGCGCTCGGC
AGCCTGGTCATCGGCACCGATCATGCCGCCGAGGCGGTGATGGGCTTCTTCACCAAATTCGGCGATGGCGCCGCCGATAT
CCTGCCGCTCGCCGGCCTCAACAAGCGCCGCGTCCGCCTGTTGGCAAAGCGGCTCGGCGCCCCTGATGAGCTGGTCTTCA
AGGTTCCCACAGCCGATCTCGAGGACCAGCGGCCGCTGCGCCCCGACGAGGAGGCCTATGGCGTCAGCTATGACGAGATC
GACGATTTCCTCGAAGGCAAGCCGGTCGGCGAGATCGCCCGCCGCCGCATCCTTGCCGCCTATCGCGCCACCGCCCACAA
GCGCGCTTTGCCGGTGGCCGTCAACGCGCTCTGA

Upstream 100 bases:

>100_bases
GGGAGATGCCCGGCAGGGCAGAGGGGGGTACCACGCACCTTACCCTTCCCATATCTTTCCGCCATAGTGCCCGCGAATCC
TACAACAGAGGAAGAACCCC

Downstream 100 bases:

>100_bases
CGGGCAGTCGACGTTACGGCCGCACCGGCGAGCCGGTCCGGCCTGCCGAGGCGAGATAGGCCTGCGGCGCGAAATAGACG
CCGTCCGCGTCGAAGGCCTC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTVLSDEQGEIIRELGVAADIDPEREIERRTEFLKDYLVASGMRGYVLGISGGVDSLTAALIAQKAVRELRESGHAAEFI
AVRLPYDVQADEADAERALATIGADRAMVVNIKAAADAMLAAAQNGGLAFADAGRQDFILGNIKARQRMIAQFALAGALG
SLVIGTDHAAEAVMGFFTKFGDGAADILPLAGLNKRRVRLLAKRLGAPDELVFKVPTADLEDQRPLRPDEEAYGVSYDEI
DDFLEGKPVGEIARRRILAAYRATAHKRALPVAVNAL

Sequences:

>Translated_277_residues
MTVLSDEQGEIIRELGVAADIDPEREIERRTEFLKDYLVASGMRGYVLGISGGVDSLTAALIAQKAVRELRESGHAAEFI
AVRLPYDVQADEADAERALATIGADRAMVVNIKAAADAMLAAAQNGGLAFADAGRQDFILGNIKARQRMIAQFALAGALG
SLVIGTDHAAEAVMGFFTKFGDGAADILPLAGLNKRRVRLLAKRLGAPDELVFKVPTADLEDQRPLRPDEEAYGVSYDEI
DDFLEGKPVGEIARRRILAAYRATAHKRALPVAVNAL
>Mature_276_residues
TVLSDEQGEIIRELGVAADIDPEREIERRTEFLKDYLVASGMRGYVLGISGGVDSLTAALIAQKAVRELRESGHAAEFIA
VRLPYDVQADEADAERALATIGADRAMVVNIKAAADAMLAAAQNGGLAFADAGRQDFILGNIKARQRMIAQFALAGALGS
LVIGTDHAAEAVMGFFTKFGDGAADILPLAGLNKRRVRLLAKRLGAPDELVFKVPTADLEDQRPLRPDEEAYGVSYDEID
DFLEGKPVGEIARRRILAAYRATAHKRALPVAVNAL

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=270, Percent_Identity=50.3703703703704, Blast_Score=244, Evalue=4e-66,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29712; Mature: 29581

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLSDEQGEIIRELGVAADIDPEREIERRTEFLKDYLVASGMRGYVLGISGGVDSLTAA
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
LIAQKAVRELRESGHAAEFIAVRLPYDVQADEADAERALATIGADRAMVVNIKAAADAML
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
AAAQNGGLAFADAGRQDFILGNIKARQRMIAQFALAGALGSLVIGTDHAAEAVMGFFTKF
HHHCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHH
GDGAADILPLAGLNKRRVRLLAKRLGAPDELVFKVPTADLEDQRPLRPDEEAYGVSYDEI
CCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCHHHH
DDFLEGKPVGEIARRRILAAYRATAHKRALPVAVNAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
TVLSDEQGEIIRELGVAADIDPEREIERRTEFLKDYLVASGMRGYVLGISGGVDSLTAA
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
LIAQKAVRELRESGHAAEFIAVRLPYDVQADEADAERALATIGADRAMVVNIKAAADAML
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
AAAQNGGLAFADAGRQDFILGNIKARQRMIAQFALAGALGSLVIGTDHAAEAVMGFFTKF
HHHCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHH
GDGAADILPLAGLNKRRVRLLAKRLGAPDELVFKVPTADLEDQRPLRPDEEAYGVSYDEI
CCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCHHHH
DDFLEGKPVGEIARRRILAAYRATAHKRALPVAVNAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA