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The map label for this gene is yafC [C]

Identifier: 209548436

GI number: 209548436

Start: 851813

End: 852715

Strand: Reverse

Name: yafC [C]

Synonym: Rleg2_0833

Alternate gene names: 209548436

Gene position: 852715-851813 (Counterclockwise)

Preceding gene: 209548441

Following gene: 209548434

Centisome position: 18.79

GC content: 60.91

Gene sequence:

>903_bases
ATGGATCGGCTGACAAGCCTCACAGTCTTTGGCCGCGTGGTGGAATGCGGCGGTTTTTCCGCCGCGGCGCGCCGGCTCAA
CATGTCGGTCACCATGGTCGGCAATCACGTGCAGTCACTGGAGGATCGGCTCGGGGTCAGGCTGCTCAACCGCACGACGC
GCAAGGTCAGCCTGACGGAAACCGGCAAATATTATTATGAACGCTCGTCGCAGATTCTTGCCGAACTCGACGAAGCCGAC
CGGACGGCCGGCGCACTCAGTACCACGCCGCGCGGCACGCTGAAGGTCTATACCAGCAGCGCCATCGTGCGTTTCCTGCT
ACCCGTCGTCAGCGAATATATGGAACTCTATCCGTCGATCGCGCTCGATTTCAGCATCGGCGAGCGGATGGTCGACATGA
TCGAGGACGGCTACGATCTGGTGATCCGCACCGTGCCGCCGCCGGATTCCTCGCTCGTCGCCCGCAAGCTGACGCCCTGG
CGCCATGTGCTCGTCTGCTCGCCGGCCTATTTCGAAAGCCATCGTATGCCGCAAAAACCGGCCGAGGTCGCCGATCACAA
TTGCCTGCAATATGCCTATTATCCCTATGGCGAGGAATGGCGCTTCGAGGATGGCGAAGGCCGGCAGGAAAGCGTCAAGA
TCGGCGGCAACGCCGTCTCCAACAGCGCCGAAATGCTGCGTTTCCTGACGCTGAACGGCCAGGGCATCTTCCTGGCGCCC
AGCTTCGTGGTCTTCGACGACATCGCCGAAGGACGGCTGGTGAAGATCATGCCGGACTACCGGCCGGTCGAATTCAGCAT
CAACGCCGTCTATCCCAACCGCAGCCATCTTGCGACCAAGGTGAGGCTGTTCATCGATCTCCTGGCGGAGAGATTTGCGG
AGCATCGGAAGTGGATGACGTGA

Upstream 100 bases:

>100_bases
CCCTGTTCGATTGAAGGGACAATGCACCCTGCCGTTCATTGTGATAATCTGCTTATATCGGGAAGGATCATCAACTCTCA
GCGGATAATCCGAGGGCAAT

Downstream 100 bases:

>100_bases
GGCGTGAGGCGGCAGACCTCAGCGCGAGAGGCTGCCCCTCACCCTAGCCCTCTCCCCGTTCTCACGGGGAGAGGGGACGT
GCCCTGCGAGACGTTGGCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDRLTSLTVFGRVVECGGFSAAARRLNMSVTMVGNHVQSLEDRLGVRLLNRTTRKVSLTETGKYYYERSSQILAELDEAD
RTAGALSTTPRGTLKVYTSSAIVRFLLPVVSEYMELYPSIALDFSIGERMVDMIEDGYDLVIRTVPPPDSSLVARKLTPW
RHVLVCSPAYFESHRMPQKPAEVADHNCLQYAYYPYGEEWRFEDGEGRQESVKIGGNAVSNSAEMLRFLTLNGQGIFLAP
SFVVFDDIAEGRLVKIMPDYRPVEFSINAVYPNRSHLATKVRLFIDLLAERFAEHRKWMT

Sequences:

>Translated_300_residues
MDRLTSLTVFGRVVECGGFSAAARRLNMSVTMVGNHVQSLEDRLGVRLLNRTTRKVSLTETGKYYYERSSQILAELDEAD
RTAGALSTTPRGTLKVYTSSAIVRFLLPVVSEYMELYPSIALDFSIGERMVDMIEDGYDLVIRTVPPPDSSLVARKLTPW
RHVLVCSPAYFESHRMPQKPAEVADHNCLQYAYYPYGEEWRFEDGEGRQESVKIGGNAVSNSAEMLRFLTLNGQGIFLAP
SFVVFDDIAEGRLVKIMPDYRPVEFSINAVYPNRSHLATKVRLFIDLLAERFAEHRKWMT
>Mature_300_residues
MDRLTSLTVFGRVVECGGFSAAARRLNMSVTMVGNHVQSLEDRLGVRLLNRTTRKVSLTETGKYYYERSSQILAELDEAD
RTAGALSTTPRGTLKVYTSSAIVRFLLPVVSEYMELYPSIALDFSIGERMVDMIEDGYDLVIRTVPPPDSSLVARKLTPW
RHVLVCSPAYFESHRMPQKPAEVADHNCLQYAYYPYGEEWRFEDGEGRQESVKIGGNAVSNSAEMLRFLTLNGQGIFLAP
SFVVFDDIAEGRLVKIMPDYRPVEFSINAVYPNRSHLATKVRLFIDLLAERFAEHRKWMT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=36.3636363636364, Blast_Score=173, Evalue=9e-45,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=34.4481605351171, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=34.3333333333333, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=32.7702702702703, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=30.8771929824561, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=27.9461279461279, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=27.9850746268657, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790011, Length=291, Percent_Identity=24.0549828178694, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788706, Length=177, Percent_Identity=30.5084745762712, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787879, Length=153, Percent_Identity=34.640522875817, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787601, Length=174, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=24.9158249158249, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=29.0780141843972, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=23.489932885906, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693105, Length=120, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=118, Percent_Identity=36.4406779661017, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33994; Mature: 33994

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLTSLTVFGRVVECGGFSAAARRLNMSVTMVGNHVQSLEDRLGVRLLNRTTRKVSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEE
TGKYYYERSSQILAELDEADRTAGALSTTPRGTLKVYTSSAIVRFLLPVVSEYMELYPSI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
ALDFSIGERMVDMIEDGYDLVIRTVPPPDSSLVARKLTPWRHVLVCSPAYFESHRMPQKP
EEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHCCCCCCC
AEVADHNCLQYAYYPYGEEWRFEDGEGRQESVKIGGNAVSNSAEMLRFLTLNGQGIFLAP
HHHHCCCCEEEEECCCCCCEECCCCCCCCCEEEECCCCCCCHHHEEEEEEECCCEEEECC
SFVVFDDIAEGRLVKIMPDYRPVEFSINAVYPNRSHLATKVRLFIDLLAERFAEHRKWMT
CEEEEECCCCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDRLTSLTVFGRVVECGGFSAAARRLNMSVTMVGNHVQSLEDRLGVRLLNRTTRKVSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEE
TGKYYYERSSQILAELDEADRTAGALSTTPRGTLKVYTSSAIVRFLLPVVSEYMELYPSI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
ALDFSIGERMVDMIEDGYDLVIRTVPPPDSSLVARKLTPWRHVLVCSPAYFESHRMPQKP
EEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHCCCCCCC
AEVADHNCLQYAYYPYGEEWRFEDGEGRQESVKIGGNAVSNSAEMLRFLTLNGQGIFLAP
HHHHCCCCEEEEECCCCCCEECCCCCCCCCEEEECCCCCCCHHHEEEEEEECCCEEEECC
SFVVFDDIAEGRLVKIMPDYRPVEFSINAVYPNRSHLATKVRLFIDLLAERFAEHRKWMT
CEEEEECCCCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]