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The map label for this gene is gcvA [H]

Identifier: 209548396

GI number: 209548396

Start: 810768

End: 811664

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_0793

Alternate gene names: 209548396

Gene position: 810768-811664 (Clockwise)

Preceding gene: 209548394

Following gene: 209548399

Centisome position: 17.87

GC content: 64.99

Gene sequence:

>897_bases
ATGAAGAACCTGAACACTGTGCATCTCAACGGCCTCAGGGCGCTGGAGGCCGTCGGTCGGCTCGGCTCGTTGCAGGCGGC
CGCCGACGAGCTCGGCGTCTCGGTCGGCGCCGTCAGCCAGCAGGTCATCAAGGCGGAGGCGCAGCTTGGCCTGGTGATCT
TCGAGCGCACCGCCCGGGGAATGATGACGACCGAAGCCGCCCGGCCAATACTGCTGGCGCTCGACGAAGGTTTTGCCCGT
CTTTCGGCGGCCGTGTCGATTGCCCAACGCAAGGACGATACGATCCTGACGATCTCGGTGGCGCCGGTTTTTGCCGCCCG
CTGGCTCGTCTGCCGGCTCGATCGTTTTGCCGAACGTCATCCCGACATCAAGCTGCGATTGGATGCGACCACCAATCTCG
TCAGTCCCGCTCTCTGCGATGTCGATATCGGCATTCGCGTCGGCGCCGGCAAATGGCCTGACGTGAAGGCCGAACTGCTC
TTGGAGCAGGAGGTTTTTCCCGTCTGCTCGCCGGAGATGGCGGCGAGATTGAAGCAGCCTTCCGATATCCTGGCGCTGCC
TGCCGTCGTCGACGGCCGCGCCATGTTCTCCTGGGATGTCTGGATGCGCGAGGCGGGCCTGTCAGGCGCGACACCTGCGA
CCCGCCACGTCTTCAACGATGCCTCGCTGTGCCTCGATGCGGCGATCGCCGGGCAAGGCGTCATGCTCGCCTGGCAGACC
CTTGCCGCCTTTGCGCTTGCCGAAGGCCGGCTGGTTGCGCCCTTCGGCATTCGCGCGCGCACCGGCTTCGGCCACTACTT
CGTCACCGCCGAAGGCACGCGCGAACCGAAGAAGGTCAGGGATTTCAAGGCTTGGATCCGCGAGGAAATGGCCGGCACGC
TTGCGCTGTTCCGATAA

Upstream 100 bases:

>100_bases
TTCTCCAAACGGCGGGGGATGATCGCCGCAGGGTGTAATCTCTGCTTCCTCAGGCTGGTTTTCAATTGAGATTAATGCGG
CTCTGCTATAGAAAAACTAT

Downstream 100 bases:

>100_bases
CTCAGACCTCGATCGGTTCCCTGCCACGCGTCTCCTGCATCCTTTTGTAGGCCGGGCGCGACTGGCAGCGCTCGATCCAG
GCCTTGATATTCGGATGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKNLNTVHLNGLRALEAVGRLGSLQAAADELGVSVGAVSQQVIKAEAQLGLVIFERTARGMMTTEAARPILLALDEGFAR
LSAAVSIAQRKDDTILTISVAPVFAARWLVCRLDRFAERHPDIKLRLDATTNLVSPALCDVDIGIRVGAGKWPDVKAELL
LEQEVFPVCSPEMAARLKQPSDILALPAVVDGRAMFSWDVWMREAGLSGATPATRHVFNDASLCLDAAIAGQGVMLAWQT
LAAFALAEGRLVAPFGIRARTGFGHYFVTAEGTREPKKVRDFKAWIREEMAGTLALFR

Sequences:

>Translated_298_residues
MKNLNTVHLNGLRALEAVGRLGSLQAAADELGVSVGAVSQQVIKAEAQLGLVIFERTARGMMTTEAARPILLALDEGFAR
LSAAVSIAQRKDDTILTISVAPVFAARWLVCRLDRFAERHPDIKLRLDATTNLVSPALCDVDIGIRVGAGKWPDVKAELL
LEQEVFPVCSPEMAARLKQPSDILALPAVVDGRAMFSWDVWMREAGLSGATPATRHVFNDASLCLDAAIAGQGVMLAWQT
LAAFALAEGRLVAPFGIRARTGFGHYFVTAEGTREPKKVRDFKAWIREEMAGTLALFR
>Mature_298_residues
MKNLNTVHLNGLRALEAVGRLGSLQAAADELGVSVGAVSQQVIKAEAQLGLVIFERTARGMMTTEAARPILLALDEGFAR
LSAAVSIAQRKDDTILTISVAPVFAARWLVCRLDRFAERHPDIKLRLDATTNLVSPALCDVDIGIRVGAGKWPDVKAELL
LEQEVFPVCSPEMAARLKQPSDILALPAVVDGRAMFSWDVWMREAGLSGATPATRHVFNDASLCLDAAIAGQGVMLAWQT
LAAFALAEGRLVAPFGIRARTGFGHYFVTAEGTREPKKVRDFKAWIREEMAGTLALFR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=34.3537414965986, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=31.2925170068027, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=27.5747508305648, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87081978, Length=265, Percent_Identity=28.6792452830189, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787128, Length=250, Percent_Identity=27.2, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32246; Mature: 32246

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLNTVHLNGLRALEAVGRLGSLQAAADELGVSVGAVSQQVIKAEAQLGLVIFERTARG
CCCCCEEEECHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECHHCC
MMTTEAARPILLALDEGFARLSAAVSIAQRKDDTILTISVAPVFAARWLVCRLDRFAERH
CEEHHCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHC
PDIKLRLDATTNLVSPALCDVDIGIRVGAGKWPDVKAELLLEQEVFPVCSPEMAARLKQP
CCEEEEEECCCCCCCCCCEEEECCEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCC
SDILALPAVVDGRAMFSWDVWMREAGLSGATPATRHVFNDASLCLDAAIAGQGVMLAWQT
CCEEEECEEECCCEEEEHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LAAFALAEGRLVAPFGIRARTGFGHYFVTAEGTREPKKVRDFKAWIREEMAGTLALFR
HHHHHHHCCCEECCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLNTVHLNGLRALEAVGRLGSLQAAADELGVSVGAVSQQVIKAEAQLGLVIFERTARG
CCCCCEEEECHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECHHCC
MMTTEAARPILLALDEGFARLSAAVSIAQRKDDTILTISVAPVFAARWLVCRLDRFAERH
CEEHHCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHC
PDIKLRLDATTNLVSPALCDVDIGIRVGAGKWPDVKAELLLEQEVFPVCSPEMAARLKQP
CCEEEEEECCCCCCCCCCEEEECCEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCC
SDILALPAVVDGRAMFSWDVWMREAGLSGATPATRHVFNDASLCLDAAIAGQGVMLAWQT
CCEEEECEEECCCEEEEHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LAAFALAEGRLVAPFGIRARTGFGHYFVTAEGTREPKKVRDFKAWIREEMAGTLALFR
HHHHHHHCCCEECCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]