Click here to switch to the map view.

The map label for this gene is thrC [H]

Identifier: 209548241

GI number: 209548241

Start: 660113

End: 661528

Strand: Reverse

Name: thrC [H]

Synonym: Rleg2_0635

Alternate gene names: 209548241

Gene position: 661528-660113 (Counterclockwise)

Preceding gene: 209548243

Following gene: 209548240

Centisome position: 14.58

GC content: 62.22

Gene sequence:

>1416_bases
GTGGACTATATCTCGACCCGCGGCGAGGCCCCTGCCCTCGGCTTTTGCGACGCCCTGCTGACGGGCCTGGCGCGCGATGG
CGGGCTCTATGTTCCCCGTGAATGGCCGCAGTTTTCGAAGAAAGAAATCCGCGCGCTGAGGGGCAAGACCTATCAGGAAA
TCGCCTTCACCATCCTTTCGCCCTTCACCAATGGCGAGATCCCCGAGGCGACCTTCCGGGCGATGATCGACGAGGCCTAC
GGCACCTTCCGCCATCCGGCGATCGCGCCGCTCGTCCAGACCGGGCCGAACAGTTTTGTCATGGAGCTGTTCCACGGCAC
GACGCTCGCCTTCAAGGATGTGGCGATGCAGCTGCTCGCACGGCTGATGGATTATGCACTTGAGAAGCGCGGCGAGCGGG
CGACGATTGTCGGCGCCACGTCGGGTGATACCGGCGGGGCGGCGATCGACGCCTTTGCCGGGCGCGAGCGCACCGACATC
TTCATTCTCTTCCCGCATGGCAAGGTCTCGCCGGTGCAGCAGCGGCAGATGACGACGTCAACGGCCGCCAATGTGCATGC
GCTTGCCGTCGAAGGCAATTTCGACGATTGCCAGAACCTGGTGAAGGCGATGTTCAACGACGCGGCGTTCCGCGACAAGG
TCAATCTCTCCGGCGTCAACTCGATCAACTGGGCGCGCATCATGGCCCAGATCGTCTATTATTTCACGACCGCGGTGGCC
TTGGGCGGGCCGGACCGGAAGATCTCGTTCACGGTGCCGACAGGCAATTTCGGCGATATCTTCGCCGGCTACTGCGCCAA
GCGCATGGGTCTGCCGATCGACCGGCTGGTCATCGCCACCAACGAAAACGACATTCTGGCGCGGACACTGAAGACCGGCC
GCTACGACATGAAGGCTGTGAAGGCAACGAGCTCGCCGTCGATGGATATCCAGATCTCGTCGAATTTCGAACGGCTGCTT
TTCGAAGCCTATGGACGCGACGCCTCGAAGGTGCGCGCGGCCATGGAAAGCCTCAAGCAATCCAACGGCTTCGAGATCGG
CCCGCAGGCATTGAAGGCGATCCGCCGCGACTTCCGGGCCGGCCGCGCCAGCGAGAAACAAGTGGCCGAGACGATCCGCA
AGACCCATGCCGAGACCGGCTATCTCCTTGATCCGCATTCGGCGATCGGCGTCTTCGTCGCCAGCAAGAAGGAAAAGCCG
AATACGCCGATGGTGACGCTTTCGACCGCGCATCCGGCGAAATTCCCCGCCGCCGTAAAATCGGCCTGCGGTATTGACCC
GGCGCTTCCGACGTGGCTTGCTGATCTGATGCACAGGGAGGAGCGCTTCCAGATCATCGAACCGGAGCTGAAAGCCGTAG
AGACCTTTATCGGCAAACACGCCCGCGGCAAGACGACGGCAGGCGCAGAAAGATAG

Upstream 100 bases:

>100_bases
ATGGTATGGCCGCAAGCGCGTCCCTCGCCGGTTTCCGGCTGCGCGAGCCACGGCCCGGCCCGGCCGAATTTCCTCAAGCG
ATAGATCAAGGATGACCAAG

Downstream 100 bases:

>100_bases
CCAATGACAGTTGAGTGCACCCGGCTGAAATCCGGGCTGACAGTAGTGACAGAAACCATGCCGCACCTTGAAAGCGTTGC
GCTCGGAGTCTGGATCAAAT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDYISTRGEAPALGFCDALLTGLARDGGLYVPREWPQFSKKEIRALRGKTYQEIAFTILSPFTNGEIPEATFRAMIDEAY
GTFRHPAIAPLVQTGPNSFVMELFHGTTLAFKDVAMQLLARLMDYALEKRGERATIVGATSGDTGGAAIDAFAGRERTDI
FILFPHGKVSPVQQRQMTTSTAANVHALAVEGNFDDCQNLVKAMFNDAAFRDKVNLSGVNSINWARIMAQIVYYFTTAVA
LGGPDRKISFTVPTGNFGDIFAGYCAKRMGLPIDRLVIATNENDILARTLKTGRYDMKAVKATSSPSMDIQISSNFERLL
FEAYGRDASKVRAAMESLKQSNGFEIGPQALKAIRRDFRAGRASEKQVAETIRKTHAETGYLLDPHSAIGVFVASKKEKP
NTPMVTLSTAHPAKFPAAVKSACGIDPALPTWLADLMHREERFQIIEPELKAVETFIGKHARGKTTAGAER

Sequences:

>Translated_471_residues
MDYISTRGEAPALGFCDALLTGLARDGGLYVPREWPQFSKKEIRALRGKTYQEIAFTILSPFTNGEIPEATFRAMIDEAY
GTFRHPAIAPLVQTGPNSFVMELFHGTTLAFKDVAMQLLARLMDYALEKRGERATIVGATSGDTGGAAIDAFAGRERTDI
FILFPHGKVSPVQQRQMTTSTAANVHALAVEGNFDDCQNLVKAMFNDAAFRDKVNLSGVNSINWARIMAQIVYYFTTAVA
LGGPDRKISFTVPTGNFGDIFAGYCAKRMGLPIDRLVIATNENDILARTLKTGRYDMKAVKATSSPSMDIQISSNFERLL
FEAYGRDASKVRAAMESLKQSNGFEIGPQALKAIRRDFRAGRASEKQVAETIRKTHAETGYLLDPHSAIGVFVASKKEKP
NTPMVTLSTAHPAKFPAAVKSACGIDPALPTWLADLMHREERFQIIEPELKAVETFIGKHARGKTTAGAER
>Mature_471_residues
MDYISTRGEAPALGFCDALLTGLARDGGLYVPREWPQFSKKEIRALRGKTYQEIAFTILSPFTNGEIPEATFRAMIDEAY
GTFRHPAIAPLVQTGPNSFVMELFHGTTLAFKDVAMQLLARLMDYALEKRGERATIVGATSGDTGGAAIDAFAGRERTDI
FILFPHGKVSPVQQRQMTTSTAANVHALAVEGNFDDCQNLVKAMFNDAAFRDKVNLSGVNSINWARIMAQIVYYFTTAVA
LGGPDRKISFTVPTGNFGDIFAGYCAKRMGLPIDRLVIATNENDILARTLKTGRYDMKAVKATSSPSMDIQISSNFERLL
FEAYGRDASKVRAAMESLKQSNGFEIGPQALKAIRRDFRAGRASEKQVAETIRKTHAETGYLLDPHSAIGVFVASKKEKP
NTPMVTLSTAHPAKFPAAVKSACGIDPALPTWLADLMHREERFQIIEPELKAVETFIGKHARGKTTAGAER

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=456, Percent_Identity=36.6228070175439, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI153792148, Length=455, Percent_Identity=35.8241758241758, Blast_Score=271, Evalue=8e-73,
Organism=Escherichia coli, GI1786185, Length=439, Percent_Identity=33.0296127562642, Blast_Score=221, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6319901, Length=457, Percent_Identity=44.6389496717724, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 51587; Mature: 51587

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYISTRGEAPALGFCDALLTGLARDGGLYVPREWPQFSKKEIRALRGKTYQEIAFTILS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHC
PFTNGEIPEATFRAMIDEAYGTFRHPAIAPLVQTGPNSFVMELFHGTTLAFKDVAMQLLA
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHH
RLMDYALEKRGERATIVGATSGDTGGAAIDAFAGRERTDIFILFPHGKVSPVQQRQMTTS
HHHHHHHHCCCCEEEEEEECCCCCCCCEEHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
TAANVHALAVEGNFDDCQNLVKAMFNDAAFRDKVNLSGVNSINWARIMAQIVYYFTTAVA
HCCCEEEEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGGPDRKISFTVPTGNFGDIFAGYCAKRMGLPIDRLVIATNENDILARTLKTGRYDMKAV
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCHHHHHHHCCCCHHHHE
KATSSPSMDIQISSNFERLLFEAYGRDASKVRAAMESLKQSNGFEIGPQALKAIRRDFRA
ECCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GRASEKQVAETIRKTHAETGYLLDPHSAIGVFVASKKEKPNTPMVTLSTAHPAKFPAAVK
CCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHH
SACGIDPALPTWLADLMHREERFQIIEPELKAVETFIGKHARGKTTAGAER
HHCCCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDYISTRGEAPALGFCDALLTGLARDGGLYVPREWPQFSKKEIRALRGKTYQEIAFTILS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHC
PFTNGEIPEATFRAMIDEAYGTFRHPAIAPLVQTGPNSFVMELFHGTTLAFKDVAMQLLA
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHH
RLMDYALEKRGERATIVGATSGDTGGAAIDAFAGRERTDIFILFPHGKVSPVQQRQMTTS
HHHHHHHHCCCCEEEEEEECCCCCCCCEEHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
TAANVHALAVEGNFDDCQNLVKAMFNDAAFRDKVNLSGVNSINWARIMAQIVYYFTTAVA
HCCCEEEEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGGPDRKISFTVPTGNFGDIFAGYCAKRMGLPIDRLVIATNENDILARTLKTGRYDMKAV
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCHHHHHHHCCCCHHHHE
KATSSPSMDIQISSNFERLLFEAYGRDASKVRAAMESLKQSNGFEIGPQALKAIRRDFRA
ECCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GRASEKQVAETIRKTHAETGYLLDPHSAIGVFVASKKEKPNTPMVTLSTAHPAKFPAAVK
CCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHH
SACGIDPALPTWLADLMHREERFQIIEPELKAVETFIGKHARGKTTAGAER
HHCCCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1333566; 10984043 [H]