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The map label for this gene is dacF [H]

Identifier: 209548202

GI number: 209548202

Start: 622347

End: 623630

Strand: Direct

Name: dacF [H]

Synonym: Rleg2_0596

Alternate gene names: 209548202

Gene position: 622347-623630 (Clockwise)

Preceding gene: 209548200

Following gene: 209548203

Centisome position: 13.71

GC content: 65.34

Gene sequence:

>1284_bases
ATGAGAACGCTTCTGGCGGCTGTTTTGACCGTAACGCTGGCCGTTTCGGCGCCTCTTGCGGCCCTGGCCGGCAGCGCTTC
ATTGGTCCTCGATGCCCGCACCGGCAAGGTTCTCGCCGCCGAAAACGCCGATACGCTGAACCATCCGGCCTCGCTGACGA
AGATGATGACGCTCTATCTCACTTTCGAAGCTTTGAAGCGCGGCAAGATCGCCTGGGATACGCCGATCAAGATGTCGAAA
TACGCCGCCTCCCGGCCGCCGACCAAGCTCGGCGTCAAGGCCGGGGGCACGATCACCGTGCGCGAAGCGGTCTATGGCAT
GATCATCAAATCCGCCAATGATGCCGCCTCCGCCATGGGCGAGAAGCTCGGCGGCTCGGAAAGCGGTTTTGCCCGGATGA
TGACGGCCAAGGCCCGCCAGCTCGGCATGAGCCGCACCGTCTTCGTCAACGCCTCCGGCCTGCCGGACGGCCGCCAGGTC
ACCACGGCGCGAGATATGTCGACGCTCGCCATCGCGCTGATGAAGAATTATCCGAACGAATACCGGCTGTTTTCGGTGGC
AAGCTTCAATTTCCGTGGCCGCACCGTGCGCGGCCACAACAATCTGATGTACCGCTACCAGGGCATGGACGGCATCAAGA
CCGGTTACACCAACGCCTCCGGCTTCAATCTCGTCAGCGCTGTTCGGGACGGCAACCGCCGCGTCGTCGGCGTCGTGCTC
GGCGGCCGCACCGCCCGCAGCCGCGACGACAAGATGGCCGGTCTGCTTGACCGTTATCTCGGCCGTGCCTCCTCTTCCGG
CGGCGCCAGGCTGGTGGCGAGCGTCGGCGCCAGGGAGCCTGTCGAAGTGGCGTCTGCCGCCGACGATTCCGATGTTCCCG
TGCCGCTCAGCGCACCGCGCCCGGCCGACGATCTTGCAGCAAAGAGCCTGGCCTATGCCGATGACGCCGTCGTGCCGCTG
GAGCGTCCGGTCGCAATGGACGAAATCCTGAACGCCGGCAAGGCGCCGAAAATGTCGGCGGAAAAGACCGATGGCGACTG
GCAGATCCAGATCTCGGCCGCCCCGAGCGCTGATGCCGCGCGCGCGCTTCTTGCCCAGGCGAAGTCCGAAGGCGGCGCGC
CGCTGGTTTCCGCCTCGCCCTATACCGAAGCGGTCGGCAAGGGCGCCAACAAGATCTACCGCGCCCGCTTCGTCGGCTTC
GCCAGCAAGGATGCAGCCGTTTCCGCCTGCGATGCACTGAAGCAGCGCTCCTACGACTGCATGCTGCTGCCGGACCACGG
CTGA

Upstream 100 bases:

>100_bases
ATGGTAGGAGTTGCCTCAAAGTCGCGCCAAACTTGCCGCTTCTGTCACGTTACGGCACAACGCCGATCCTATCGAGCGCC
GACGCATCAAGGGGAACTGC

Downstream 100 bases:

>100_bases
TCGCCGCAGGAATTCAAAGTGTTACAGGGTCCTTTGCGCGTTTGAACAAACGCGCGGCGCTGTAAGACAATCGACTCTGG
ACAAGCATCGGGAATCAGCG

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRTLLAAVLTVTLAVSAPLAALAGSASLVLDARTGKVLAAENADTLNHPASLTKMMTLYLTFEALKRGKIAWDTPIKMSK
YAASRPPTKLGVKAGGTITVREAVYGMIIKSANDAASAMGEKLGGSESGFARMMTAKARQLGMSRTVFVNASGLPDGRQV
TTARDMSTLAIALMKNYPNEYRLFSVASFNFRGRTVRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAVRDGNRRVVGVVL
GGRTARSRDDKMAGLLDRYLGRASSSGGARLVASVGAREPVEVASAADDSDVPVPLSAPRPADDLAAKSLAYADDAVVPL
ERPVAMDEILNAGKAPKMSAEKTDGDWQIQISAAPSADAARALLAQAKSEGGAPLVSASPYTEAVGKGANKIYRARFVGF
ASKDAAVSACDALKQRSYDCMLLPDHG

Sequences:

>Translated_427_residues
MRTLLAAVLTVTLAVSAPLAALAGSASLVLDARTGKVLAAENADTLNHPASLTKMMTLYLTFEALKRGKIAWDTPIKMSK
YAASRPPTKLGVKAGGTITVREAVYGMIIKSANDAASAMGEKLGGSESGFARMMTAKARQLGMSRTVFVNASGLPDGRQV
TTARDMSTLAIALMKNYPNEYRLFSVASFNFRGRTVRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAVRDGNRRVVGVVL
GGRTARSRDDKMAGLLDRYLGRASSSGGARLVASVGAREPVEVASAADDSDVPVPLSAPRPADDLAAKSLAYADDAVVPL
ERPVAMDEILNAGKAPKMSAEKTDGDWQIQISAAPSADAARALLAQAKSEGGAPLVSASPYTEAVGKGANKIYRARFVGF
ASKDAAVSACDALKQRSYDCMLLPDHG
>Mature_427_residues
MRTLLAAVLTVTLAVSAPLAALAGSASLVLDARTGKVLAAENADTLNHPASLTKMMTLYLTFEALKRGKIAWDTPIKMSK
YAASRPPTKLGVKAGGTITVREAVYGMIIKSANDAASAMGEKLGGSESGFARMMTAKARQLGMSRTVFVNASGLPDGRQV
TTARDMSTLAIALMKNYPNEYRLFSVASFNFRGRTVRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAVRDGNRRVVGVVL
GGRTARSRDDKMAGLLDRYLGRASSSGGARLVASVGAREPVEVASAADDSDVPVPLSAPRPADDLAAKSLAYADDAVVPL
ERPVAMDEILNAGKAPKMSAEKTDGDWQIQISAAPSADAARALLAQAKSEGGAPLVSASPYTEAVGKGANKIYRARFVGF
ASKDAAVSACDALKQRSYDCMLLPDHG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=316, Percent_Identity=32.5949367088608, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787062, Length=249, Percent_Identity=35.7429718875502, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87082022, Length=246, Percent_Identity=32.9268292682927, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082054, Length=230, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44846; Mature: 44846

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLLAAVLTVTLAVSAPLAALAGSASLVLDARTGKVLAAENADTLNHPASLTKMMTLYL
CHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
TFEALKRGKIAWDTPIKMSKYAASRPPTKLGVKAGGTITVREAVYGMIIKSANDAASAMG
HHHHHHCCCEEECCCCCHHHHHCCCCCCEEEECCCCEEEEHHHHHHHHHCCCCHHHHHHH
EKLGGSESGFARMMTAKARQLGMSRTVFVNASGLPDGRQVTTARDMSTLAIALMKNYPNE
HHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCC
YRLFSVASFNFRGRTVRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAVRDGNRRVVGVVL
EEEEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEE
GGRTARSRDDKMAGLLDRYLGRASSSGGARLVASVGAREPVEVASAADDSDVPVPLSAPR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
PADDLAAKSLAYADDAVVPLERPVAMDEILNAGKAPKMSAEKTDGDWQIQISAAPSADAA
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
RALLAQAKSEGGAPLVSASPYTEAVGKGANKIYRARFVGFASKDAAVSACDALKQRSYDC
HHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCE
MLLPDHG
EEECCCC
>Mature Secondary Structure
MRTLLAAVLTVTLAVSAPLAALAGSASLVLDARTGKVLAAENADTLNHPASLTKMMTLYL
CHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
TFEALKRGKIAWDTPIKMSKYAASRPPTKLGVKAGGTITVREAVYGMIIKSANDAASAMG
HHHHHHCCCEEECCCCCHHHHHCCCCCCEEEECCCCEEEEHHHHHHHHHCCCCHHHHHHH
EKLGGSESGFARMMTAKARQLGMSRTVFVNASGLPDGRQVTTARDMSTLAIALMKNYPNE
HHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCC
YRLFSVASFNFRGRTVRGHNNLMYRYQGMDGIKTGYTNASGFNLVSAVRDGNRRVVGVVL
EEEEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEE
GGRTARSRDDKMAGLLDRYLGRASSSGGARLVASVGAREPVEVASAADDSDVPVPLSAPR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
PADDLAAKSLAYADDAVVPLERPVAMDEILNAGKAPKMSAEKTDGDWQIQISAAPSADAA
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
RALLAQAKSEGGAPLVSASPYTEAVGKGANKIYRARFVGFASKDAAVSACDALKQRSYDC
HHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCE
MLLPDHG
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]