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The map label for this gene is ispB [H]

Identifier: 209548129

GI number: 209548129

Start: 537681

End: 538697

Strand: Reverse

Name: ispB [H]

Synonym: Rleg2_0523

Alternate gene names: 209548129

Gene position: 538697-537681 (Counterclockwise)

Preceding gene: 209548132

Following gene: 209548128

Centisome position: 11.87

GC content: 62.83

Gene sequence:

>1017_bases
TTGGGCGTGGTCATACCGCTTGAAGAAAGCAAAAACAAACTGGCATCCATCAAGCCATTGGTCGATCTCACCAGGGCGGA
TATGGAGCGGGTGAACCAGCTCATTCTGTCCAAGGCCGGCTCCGACGTGCAGATGATCCCCGAAGTGGCGAACCATCTGA
TCTCCTCAGGCGGCAAGCGGCTGCGGCCGATGCTGACGCTGGCGGCCGCCTCGCTGTTTGACTACCGCGGCGAAAACCAC
GTCAAGCTCGCCACCTCGGTCGAGTTCATGCACACGGCAACCCTGCTGCATGACGACGTCGTCGACGAAAGCGACCTGCG
CCGCGGCAAATCGACAGCGCGGATGATCTGGGGCAACCAGGCAAGCGTGCTGGTCGGCGACTTCCTGCTCGGCCAGGCCT
TCCGCATGATGGTCGATGTCGGCTCGCTCGATGCGCTCGACGTGCTGTCTTCCGCCGCCTGCATCATTGCCGAAGGCGAG
GTGCTGCAGCTTTCCGTCGCCAAGAACATGGAGACGACGGAGGACGATTATCTCTCGGTCATCCGCGCCAAGACGGCGGC
GCTGTTTGCCGCCGCCGCCGAAGTCGGGCCGATCGTCGCCGAGGCCGGCAAATCCGGCCGCAACGCGCTGAAATCCTACG
GCATGAATCTGGGGCTCGCCTTCCAGCTGGTCGACGATGCGCTCGATTACGGCGGCAAGGCGGCCGATCTCGGCAAGAAT
GTCGGCGACGATTTCCGCGAGGGCAAGATTACCCTGCCGGTCATTCTCGCCTATCGCCGCGGCACCGAGGACGAGCGCGC
CTTCTGGCGCGATGCGATCGAAGCCGGCAACAGCACCGATGCCAACCTCGAAAAAGCGCTGGGGCTGATCACCAAATACG
GCACGCTTTCCGACACGATCGGCCGGGCGGTCCATTACGGCACGATCGCACGGGATGCGCTGGCGCCGCTGCCCGATACG
GTCTGGAAATCCTCCCTGATGGAAGTGATCGACTTCTGCATCGAGCGCGTCAATTGA

Upstream 100 bases:

>100_bases
GTTTTCCTTCCAATTTCGCCTTGCGCGACAATTCACCCCTTGCCGCGTCCATCGCCCCTTTCTATTGTCAGATGCAAAGA
ATGAACAGGAGTCCGGGTCG

Downstream 100 bases:

>100_bases
TCAAACGTCCTTGATCTTAGGCTGAATTTCTTGCGGCGCCGCTTCAAAAAAGCCATCCTGTTATGAATCAGTGAACACAA
CTGGTTTTGCGACCGGCACC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGVVIPLEESKNKLASIKPLVDLTRADMERVNQLILSKAGSDVQMIPEVANHLISSGGKRLRPMLTLAAASLFDYRGENH
VKLATSVEFMHTATLLHDDVVDESDLRRGKSTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSSAACIIAEGE
VLQLSVAKNMETTEDDYLSVIRAKTAALFAAAAEVGPIVAEAGKSGRNALKSYGMNLGLAFQLVDDALDYGGKAADLGKN
VGDDFREGKITLPVILAYRRGTEDERAFWRDAIEAGNSTDANLEKALGLITKYGTLSDTIGRAVHYGTIARDALAPLPDT
VWKSSLMEVIDFCIERVN

Sequences:

>Translated_338_residues
MGVVIPLEESKNKLASIKPLVDLTRADMERVNQLILSKAGSDVQMIPEVANHLISSGGKRLRPMLTLAAASLFDYRGENH
VKLATSVEFMHTATLLHDDVVDESDLRRGKSTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSSAACIIAEGE
VLQLSVAKNMETTEDDYLSVIRAKTAALFAAAAEVGPIVAEAGKSGRNALKSYGMNLGLAFQLVDDALDYGGKAADLGKN
VGDDFREGKITLPVILAYRRGTEDERAFWRDAIEAGNSTDANLEKALGLITKYGTLSDTIGRAVHYGTIARDALAPLPDT
VWKSSLMEVIDFCIERVN
>Mature_337_residues
GVVIPLEESKNKLASIKPLVDLTRADMERVNQLILSKAGSDVQMIPEVANHLISSGGKRLRPMLTLAAASLFDYRGENHV
KLATSVEFMHTATLLHDDVVDESDLRRGKSTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSSAACIIAEGEV
LQLSVAKNMETTEDDYLSVIRAKTAALFAAAAEVGPIVAEAGKSGRNALKSYGMNLGLAFQLVDDALDYGGKAADLGKNV
GDDFREGKITLPVILAYRRGTEDERAFWRDAIEAGNSTDANLEKALGLITKYGTLSDTIGRAVHYGTIARDALAPLPDTV
WKSSLMEVIDFCIERVN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=223, Percent_Identity=31.390134529148, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4758430, Length=225, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI83700220, Length=225, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=47.0404984423676, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1786623, Length=233, Percent_Identity=36.480686695279, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=190, Percent_Identity=36.8421052631579, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=296, Percent_Identity=29.0540540540541, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=230, Percent_Identity=22.6086956521739, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651612, Length=311, Percent_Identity=30.8681672025724, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI281365769, Length=267, Percent_Identity=25.8426966292135, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660002, Length=267, Percent_Identity=25.8426966292135, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36459; Mature: 36328

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVIPLEESKNKLASIKPLVDLTRADMERVNQLILSKAGSDVQMIPEVANHLISSGGKR
CCEEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLAAASLFDYRGENHVKLATSVEFMHTATLLHDDVVDESDLRRGKSTARMIWGNQ
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHEEECCC
ASVLVGDFLLGQAFRMMVDVGSLDALDVLSSAACIIAEGEVLQLSVAKNMETTEDDYLSV
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCEEEEEECCCCCCCHHHHHHH
IRAKTAALFAAAAEVGPIVAEAGKSGRNALKSYGMNLGLAFQLVDDALDYGGKAADLGKN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCC
VGDDFREGKITLPVILAYRRGTEDERAFWRDAIEAGNSTDANLEKALGLITKYGTLSDTI
CCCHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
GRAVHYGTIARDALAPLPDTVWKSSLMEVIDFCIERVN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GVVIPLEESKNKLASIKPLVDLTRADMERVNQLILSKAGSDVQMIPEVANHLISSGGKR
CEEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLAAASLFDYRGENHVKLATSVEFMHTATLLHDDVVDESDLRRGKSTARMIWGNQ
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHEEECCC
ASVLVGDFLLGQAFRMMVDVGSLDALDVLSSAACIIAEGEVLQLSVAKNMETTEDDYLSV
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCEEEEEECCCCCCCHHHHHHH
IRAKTAALFAAAAEVGPIVAEAGKSGRNALKSYGMNLGLAFQLVDDALDYGGKAADLGKN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCC
VGDDFREGKITLPVILAYRRGTEDERAFWRDAIEAGNSTDANLEKALGLITKYGTLSDTI
CCCHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
GRAVHYGTIARDALAPLPDTVWKSSLMEVIDFCIERVN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]