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The map label for this gene is nemA [H]

Identifier: 209548088

GI number: 209548088

Start: 493242

End: 494351

Strand: Direct

Name: nemA [H]

Synonym: Rleg2_0481

Alternate gene names: 209548088

Gene position: 493242-494351 (Clockwise)

Preceding gene: 209548086

Following gene: 209548089

Centisome position: 10.87

GC content: 62.25

Gene sequence:

>1110_bases
ATGCACCGGCCCTCACTTTTCTCGTCACTTCATTTGGGGCGCTATCAATTGGCTCACCGTGTGGTGATGGCGCCGCTGAC
GCGCATGCGTGCCGATGCGGGCAACGTGCCAAACGCGCTGGCGCCGGAATATTACGGTCAGCGCGCCTCTTCGGGCGGAT
TGATCATCGCCGAAGCCACCCAGGTCACACCGTATGGACAGGGCTATCCATCAACACCCGGCATCCATTCCGATGAACAG
GTCAGGGGCTGGAGGAAGGTCACCGATGCGGTCCATGCCAAGGGTGGTCTCATCTTCCTGCAGCTCTGGCACGTCGGACG
CGCGTCGCATGCGAGCTTCCAGCCGAATGGTGTGTTGCCGGTCGCGCCTTCGGCGATCGCAATCACCGACCAGATGTCGC
TGACCCCCGAATGGAAGCAAGTCCCCTACGAAACGCCGCGGGCACTGGAGCTGGACGAGATCCCCGAGATCGTCGAGAAC
TATCTCCAGGCTGCGCGCAATGCCATGGCCGCTGGTTTTGACGGCGTGGAACTGCACGGCGCCAATGGCTATCTGCTTGA
GCAGTTCCTGCACGACCGCTCCAACAAGCGCACCGACATTTACGGCGGATCGATCGAAAACCGCGCGCGCCTGCTTCTGG
ATGTTACGAAAGCCGTTGTCGAAATCTGGGGTTCCGATCGCGTCGGCGTCCGGCTGTCGCCCTTCGGCACCTATAACGAC
GTCGGCGACTCCGATCCGATCGGGCTCTATAGCCATGTCCTGTCGCGCTTGATGGAACTCGACATCGCCTATGTCAGCCT
GATCGAGGCGCGCCCTGGCGCCGGAATGGAGATCGATGCCCCGCAGGCAGTCGATCAGCTTCGCCCCTTCTGGCCTAAGG
CCTTAATTCTAGCCGGCGGTTTCACCCGGCAGTCGGCGGAAGAAACCATTCGTTCGGGCCGCGCCGACGCCGTCGCCTTC
GGACGCCAGTTCATCGCCAATCCTGACCTGCCCCTTCGGCTGAAGCTGGGAGCGCCGCTCAATTCCCATGACCGCTCGAC
ATTCTATGGCGGCGGTGCAGCCGGTTACGTCGATTACCCGGAGCTGGAAGCCGCACACGCGGCGGAATGA

Upstream 100 bases:

>100_bases
CGGTCATATTAAATTAACCCAATCGGGCTGTCTGGTTGAATAAGCCGTACGGACCGAGTTCTCGCGCCCTCGGGCGCTCC
GTTTATGCAAAGGATGTCTG

Downstream 100 bases:

>100_bases
TGACGCGGCCAGCACCAACGTTGGGCGACGTCAGCGCCCGGTATCTCAACAACATCAGAGCAATATCAAAAGGAACCTTC
CCCATGAAAGTCGTTGTCTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MHRPSLFSSLHLGRYQLAHRVVMAPLTRMRADAGNVPNALAPEYYGQRASSGGLIIAEATQVTPYGQGYPSTPGIHSDEQ
VRGWRKVTDAVHAKGGLIFLQLWHVGRASHASFQPNGVLPVAPSAIAITDQMSLTPEWKQVPYETPRALELDEIPEIVEN
YLQAARNAMAAGFDGVELHGANGYLLEQFLHDRSNKRTDIYGGSIENRARLLLDVTKAVVEIWGSDRVGVRLSPFGTYND
VGDSDPIGLYSHVLSRLMELDIAYVSLIEARPGAGMEIDAPQAVDQLRPFWPKALILAGGFTRQSAEETIRSGRADAVAF
GRQFIANPDLPLRLKLGAPLNSHDRSTFYGGGAAGYVDYPELEAAHAAE

Sequences:

>Translated_369_residues
MHRPSLFSSLHLGRYQLAHRVVMAPLTRMRADAGNVPNALAPEYYGQRASSGGLIIAEATQVTPYGQGYPSTPGIHSDEQ
VRGWRKVTDAVHAKGGLIFLQLWHVGRASHASFQPNGVLPVAPSAIAITDQMSLTPEWKQVPYETPRALELDEIPEIVEN
YLQAARNAMAAGFDGVELHGANGYLLEQFLHDRSNKRTDIYGGSIENRARLLLDVTKAVVEIWGSDRVGVRLSPFGTYND
VGDSDPIGLYSHVLSRLMELDIAYVSLIEARPGAGMEIDAPQAVDQLRPFWPKALILAGGFTRQSAEETIRSGRADAVAF
GRQFIANPDLPLRLKLGAPLNSHDRSTFYGGGAAGYVDYPELEAAHAAE
>Mature_369_residues
MHRPSLFSSLHLGRYQLAHRVVMAPLTRMRADAGNVPNALAPEYYGQRASSGGLIIAEATQVTPYGQGYPSTPGIHSDEQ
VRGWRKVTDAVHAKGGLIFLQLWHVGRASHASFQPNGVLPVAPSAIAITDQMSLTPEWKQVPYETPRALELDEIPEIVEN
YLQAARNAMAAGFDGVELHGANGYLLEQFLHDRSNKRTDIYGGSIENRARLLLDVTKAVVEIWGSDRVGVRLSPFGTYND
VGDSDPIGLYSHVLSRLMELDIAYVSLIEARPGAGMEIDAPQAVDQLRPFWPKALILAGGFTRQSAEETIRSGRADAVAF
GRQFIANPDLPLRLKLGAPLNSHDRSTFYGGGAAGYVDYPELEAAHAAE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=49.0514905149051, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789463, Length=244, Percent_Identity=35.2459016393443, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17565138, Length=326, Percent_Identity=29.1411042944785, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=255, Percent_Identity=31.3725490196078, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=276, Percent_Identity=29.7101449275362, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=258, Percent_Identity=31.7829457364341, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=272, Percent_Identity=28.3088235294118, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=260, Percent_Identity=29.6153846153846, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=322, Percent_Identity=26.3975155279503, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=41.994750656168, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=40, Blast_Score=244, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40205; Mature: 40205

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRPSLFSSLHLGRYQLAHRVVMAPLTRMRADAGNVPNALAPEYYGQRASSGGLIIAEAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEECC
QVTPYGQGYPSTPGIHSDEQVRGWRKVTDAVHAKGGLIFLQLWHVGRASHASFQPNGVLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEE
VAPSAIAITDQMSLTPEWKQVPYETPRALELDEIPEIVENYLQAARNAMAAGFDGVELHG
CCCCEEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLLEQFLHDRSNKRTDIYGGSIENRARLLLDVTKAVVEIWGSDRVGVRLSPFGTYND
CCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
VGDSDPIGLYSHVLSRLMELDIAYVSLIEARPGAGMEIDAPQAVDQLRPFWPKALILAGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEECC
FTRQSAEETIRSGRADAVAFGRQFIANPDLPLRLKLGAPLNSHDRSTFYGGGAAGYVDYP
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCC
ELEAAHAAE
CCCHHHCCC
>Mature Secondary Structure
MHRPSLFSSLHLGRYQLAHRVVMAPLTRMRADAGNVPNALAPEYYGQRASSGGLIIAEAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEECC
QVTPYGQGYPSTPGIHSDEQVRGWRKVTDAVHAKGGLIFLQLWHVGRASHASFQPNGVLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEE
VAPSAIAITDQMSLTPEWKQVPYETPRALELDEIPEIVENYLQAARNAMAAGFDGVELHG
CCCCEEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLLEQFLHDRSNKRTDIYGGSIENRARLLLDVTKAVVEIWGSDRVGVRLSPFGTYND
CCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
VGDSDPIGLYSHVLSRLMELDIAYVSLIEARPGAGMEIDAPQAVDQLRPFWPKALILAGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEECC
FTRQSAEETIRSGRADAVAFGRQFIANPDLPLRLKLGAPLNSHDRSTFYGGGAAGYVDYP
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCC
ELEAAHAAE
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]