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The map label for this gene is hisS [H]

Identifier: 209548071

GI number: 209548071

Start: 473927

End: 475450

Strand: Direct

Name: hisS [H]

Synonym: Rleg2_0464

Alternate gene names: 209548071

Gene position: 473927-475450 (Clockwise)

Preceding gene: 209548067

Following gene: 209548072

Centisome position: 10.44

GC content: 63.91

Gene sequence:

>1524_bases
ATGAACGACAAGCAGAAGAAACCGCAAAAGCTCAAGGCCCGCCTGCCGCGCGGCTTCGTCGACCGGACGGCCGGCGATAT
CCGCGCCGTCAACGAGATGACCGCAAAGATCCGGGAAGTCTATGAGCATTATGGTTTCGACCCGCTCGAAACGCCGCAGT
TCGAATATACCGATGCGCTCGGCAAGTTCCTGCCGGACAGCGACCGGCCGAACGAGGGCGTCTTCTCGTTGCAGGACGAC
GACGAGCAATGGATGTCGCTGCGTTACGACCTGACGGCGCCGCTTGCCCGTCATGTCGCCGAGAATTTCAACGAGATCCA
GCTTCCCTACCGCACCTATCGTGCCGGTTACGTCTTCCGCAACGAGAAGCCGGGCCCCGGCCGCTTCCGTCAGTTCATGC
AGTTCGATGCCGACACCGTCGGCGCGCCTGGTGTTCAGGCCGATGCGGAAATGTGCATGATGATGGCCGATACGCTTGAG
GCTCTCGGCATCAAGCGCGGCGACTATGTCGTCCGCGTCAACAACCGCAAGGTTCTGGACGGCGTGCTCGAGGCGATCGG
CCTCGGCGGCGACGACAAGGCCGGCCAGCGGCTCAACGTGCTGCGCGCCATCGACAAGCTCGACAAGTTCGGCCCTGAGG
GTGTGGCCCTGCTGCTCGGTCCCGGCCGCAAGGATGAATCCGGCGACTTCACCAAGGGCGCCGGCCTCGACCAGGCGCAG
ATCGACAAGGTGCTGTTCTTCGTCGGCATCAAGGATTATGCCGAAAGCGCTTCACGCCTCGCCGAACTCGTTGCCGGAAC
CTCCAAGGGCGCCGAAGGCGTCGAGGAGCTGAACTTCATCGGTACGCTGGTTGCGAGCGCCGGCTACGGCGCCGATCGCA
TCAAGATCGATCCCTCCATCGTGCGCGGCCTCGAATATTATACCGGTCCGGTCTATGAGGCCGAACTGAGTTTCGACGTC
ACCAATGAGAAGGGCGAAAAGGTCGTCTTCGGTTCGGTCGGCGGCGGCGGCCGTTATGATGGCCTGGTCTCGCGCTTCAT
GGGCCAGCCGGTGCCGGCCACTGGCTTTTCGATCGGCGTCTCCCGGCTGATGACGGCGCTGAAGAACCTCGGCAAGCTCG
GCGCCAGCGAGGTGATCGAGCCGGTGCTGGTGACCGTCATGGACGGCGATGTCGAGGCGATGGGCCGTTATCAGAAAATG
ACGCAGGACCTGCGCGCCGCCGGCATCCGCGCCGAGATGTTCCAGGGCAACTGGAAGAAATTCGGCAACCAGCTGAAATA
TGCCGACCGCCGCGGCTGCCCCGTCGCCATCATCCAGGGGGGCGATGAGCGCGCCACCGGCGTGGTGCAGATCAAGGATC
TGATCGAGGGCAAGCGGCTCTCCGGCGAGATCGAGGACAATGCCAGCTGGCGCGAAGCCCGCGTGGCGCAGGAGACGGTG
CCGGAAGCCGACCTCGTCGCCAAGGTGAGGGAGATTCTTGCGGCGCAGGCGGAGGATCGGAAGAGGGCGGGCGGCGATGT
CTGA

Upstream 100 bases:

>100_bases
CAATCGATTCTGCTTGCGGCATCAAGACCCTTCGCCCTTGCCGCTCCCCGCCCAATCCGCTAGGAAACCGCCACTGTCAC
AGTCAGCGGAATTCCCGGAA

Downstream 100 bases:

>100_bases
GGCATACCCCCCTCTGCCCTGCCGGGCATCTCCCCCACAAGGGGGGAGATCGAATAGGAGCGGCCCCTTGCTCCAGTCAA
ACGTCGCAAATGGCCGAAAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNDKQKKPQKLKARLPRGFVDRTAGDIRAVNEMTAKIREVYEHYGFDPLETPQFEYTDALGKFLPDSDRPNEGVFSLQDD
DEQWMSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPGVQADAEMCMMMADTLE
ALGIKRGDYVVRVNNRKVLDGVLEAIGLGGDDKAGQRLNVLRAIDKLDKFGPEGVALLLGPGRKDESGDFTKGAGLDQAQ
IDKVLFFVGIKDYAESASRLAELVAGTSKGAEGVEELNFIGTLVASAGYGADRIKIDPSIVRGLEYYTGPVYEAELSFDV
TNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGASEVIEPVLVTVMDGDVEAMGRYQKM
TQDLRAAGIRAEMFQGNWKKFGNQLKYADRRGCPVAIIQGGDERATGVVQIKDLIEGKRLSGEIEDNASWREARVAQETV
PEADLVAKVREILAAQAEDRKRAGGDV

Sequences:

>Translated_507_residues
MNDKQKKPQKLKARLPRGFVDRTAGDIRAVNEMTAKIREVYEHYGFDPLETPQFEYTDALGKFLPDSDRPNEGVFSLQDD
DEQWMSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPGVQADAEMCMMMADTLE
ALGIKRGDYVVRVNNRKVLDGVLEAIGLGGDDKAGQRLNVLRAIDKLDKFGPEGVALLLGPGRKDESGDFTKGAGLDQAQ
IDKVLFFVGIKDYAESASRLAELVAGTSKGAEGVEELNFIGTLVASAGYGADRIKIDPSIVRGLEYYTGPVYEAELSFDV
TNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGASEVIEPVLVTVMDGDVEAMGRYQKM
TQDLRAAGIRAEMFQGNWKKFGNQLKYADRRGCPVAIIQGGDERATGVVQIKDLIEGKRLSGEIEDNASWREARVAQETV
PEADLVAKVREILAAQAEDRKRAGGDV
>Mature_507_residues
MNDKQKKPQKLKARLPRGFVDRTAGDIRAVNEMTAKIREVYEHYGFDPLETPQFEYTDALGKFLPDSDRPNEGVFSLQDD
DEQWMSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPGVQADAEMCMMMADTLE
ALGIKRGDYVVRVNNRKVLDGVLEAIGLGGDDKAGQRLNVLRAIDKLDKFGPEGVALLLGPGRKDESGDFTKGAGLDQAQ
IDKVLFFVGIKDYAESASRLAELVAGTSKGAEGVEELNFIGTLVASAGYGADRIKIDPSIVRGLEYYTGPVYEAELSFDV
TNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGVSRLMTALKNLGKLGASEVIEPVLVTVMDGDVEAMGRYQKM
TQDLRAAGIRAEMFQGNWKKFGNQLKYADRRGCPVAIIQGGDERATGVVQIKDLIEGKRLSGEIEDNASWREARVAQETV
PEADLVAKVREILAAQAEDRKRAGGDV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=491, Percent_Identity=29.1242362525458, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI6996014, Length=484, Percent_Identity=30.1652892561983, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1788861, Length=460, Percent_Identity=28.2608695652174, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=29.2735042735043, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=29.2735042735043, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325290, Length=531, Percent_Identity=29.1902071563089, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24643061, Length=491, Percent_Identity=30.5498981670061, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24643059, Length=510, Percent_Identity=29.2156862745098, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 55576; Mature: 55576

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKQKKPQKLKARLPRGFVDRTAGDIRAVNEMTAKIREVYEHYGFDPLETPQFEYTDAL
CCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
GKFLPDSDRPNEGVFSLQDDDEQWMSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFR
HHHCCCCCCCCCCEEEECCCHHHHHHHHHCHHHHHHHHHHCCCCEEECCHHHHHCCEEEE
NEKPGPGRFRQFMQFDADTVGAPGVQADAEMCMMMADTLEALGIKRGDYVVRVNNRKVLD
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGLGGDDKAGQRLNVLRAIDKLDKFGPEGVALLLGPGRKDESGDFTKGAGLDQAQ
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHH
IDKVLFFVGIKDYAESASRLAELVAGTSKGAEGVEELNFIGTLVASAGYGADRIKIDPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
VRGLEYYTGPVYEAELSFDVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGV
HHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHH
SRLMTALKNLGKLGASEVIEPVLVTVMDGDVEAMGRYQKMTQDLRAAGIRAEMFQGNWKK
HHHHHHHHHHHCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHH
FGNQLKYADRRGCPVAIIQGGDERATGVVQIKDLIEGKRLSGEIEDNASWREARVAQETV
HCCHHCCCCCCCCCEEEEECCCCCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
PEADLVAKVREILAAQAEDRKRAGGDV
CHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDKQKKPQKLKARLPRGFVDRTAGDIRAVNEMTAKIREVYEHYGFDPLETPQFEYTDAL
CCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
GKFLPDSDRPNEGVFSLQDDDEQWMSLRYDLTAPLARHVAENFNEIQLPYRTYRAGYVFR
HHHCCCCCCCCCCEEEECCCHHHHHHHHHCHHHHHHHHHHCCCCEEECCHHHHHCCEEEE
NEKPGPGRFRQFMQFDADTVGAPGVQADAEMCMMMADTLEALGIKRGDYVVRVNNRKVLD
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGLGGDDKAGQRLNVLRAIDKLDKFGPEGVALLLGPGRKDESGDFTKGAGLDQAQ
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHH
IDKVLFFVGIKDYAESASRLAELVAGTSKGAEGVEELNFIGTLVASAGYGADRIKIDPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
VRGLEYYTGPVYEAELSFDVTNEKGEKVVFGSVGGGGRYDGLVSRFMGQPVPATGFSIGV
HHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHH
SRLMTALKNLGKLGASEVIEPVLVTVMDGDVEAMGRYQKMTQDLRAAGIRAEMFQGNWKK
HHHHHHHHHHHCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHH
FGNQLKYADRRGCPVAIIQGGDERATGVVQIKDLIEGKRLSGEIEDNASWREARVAQETV
HCCHHCCCCCCCCCEEEEECCCCCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
PEADLVAKVREILAAQAEDRKRAGGDV
CHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA