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The map label for this gene is glcD [H]

Identifier: 209548057

GI number: 209548057

Start: 461317

End: 462756

Strand: Direct

Name: glcD [H]

Synonym: Rleg2_0450

Alternate gene names: 209548057

Gene position: 461317-462756 (Clockwise)

Preceding gene: 209548055

Following gene: 209548058

Centisome position: 10.17

GC content: 64.1

Gene sequence:

>1440_bases
ATGTCCGACGCCATCCCGTTTCTCAAACCCCGAGCCGATGTTCTCGCCCGCCGAGCCCAGATCGTCGCGGATCTCACGGA
TCTTCTGCCGCCGGAATCGCTGGTGCATGAGGCGCGTGAACTGGTTCCCTTCGAGACCGACGCCTTCGTATCCTATCGTC
GCCTGCCGCTCGCGGTCGCCCTGCCGCGCAGCACGGCTGAGGTTTCGGCGGTCATGCGTTATTGCCACCGCTACGGCATT
CCCGTCGTGCCGCGCGGCGCCGGCACCTCGCTGTCGGGCGGCGCCATTCCGCAGGAAGATGCCGTCGTGCTCGGCCTGTC
GAAAATGAACCACATCCTCGAGATCGATCTGCCCAATCGCGTTGCCGTGGTTCAGGCCGGTGTCACCAATCTCAATATTT
CAGAGTCCGTCTCGGCGGACGGCTTTTTCTACGCGCCTGATCCGAGTTCGCAGCTCGCCTGCACCATCGGCGGCAATATC
GGCATGAATTCCGGTGGTGCCCATTGCCTGAAATACGGCGTCACCACCAACAATCTGCTCGGCGTCAGGATGGTGCTGGT
CGACGGCACCGTGATCGAGCTTGGCGGCAAGGCGCTGGATGCGGCGGGTTACGACCTGCTCGGCCTCGTCTGCGGCCATG
AAGGCCAGCTCGGCATCGTCACCGAGGCGACCGTGCGGCTGATCGCCAAGCCGGAAGGCGCACGCCCCGTGCTCTTCGGC
TTCGAAGCTTCGGAAGAGGCCGGCGCCTGTGTTGCCGATGTCATCGCCGCCGGCATCATCCCCGTCGCGATCGAATTCAT
GGACAAGCCGGCAATCGAGATCTGCGAAGCCTTTGCCCATGCCGGTTATCCCCTCGATGTCGGCGCGCTGCTGATCGTCG
AGGTCGAAGGCTCGGAAGCGGAAATGGACGCCTCGCTGAAGGACATTGTCGAAATCGCCCGCCGGCATGGCGTCAAGACG
GTACGCGAATGCCAGTCGGCCACCGAAGCAGCGCTGATCTGGAAGGGCCGCAAATCCGCCTTCGGCGCCACCGGCCGCAT
CGCCGACTATATCTGCATGGACGGCACCGTGCCGCTCAGCCAGCTGTCGCATGTGCTGAAAAGAACTGCCGAGATCATCG
ACCATTACGGGTTGCGTGTCGCCAACGTCTTCCATGCCGGCGACGGCAACATGCATCCGCTGATCCTGTTCAACGCCAAC
GATCCCGAGGATGCCGCCCGCGCCGAGGCTGCCGGCAACGATATCCTCAAGCTCTGTGTCGACGCCGGCGGCTGCCTCAC
CGGCGAACATGGCGTCGGCATCGAGAAACGCGACCTGATGCGCCACCAATATTCCGAGACCGATCTCGCCCAGCAGATGG
CGGCGCGCGCTGCCTTCGATCCCGGCTGGCTGCTCAATCCGTCCAAGGTCTTCCCGCTCGAGGGGCGCCCCGCCGCATGA

Upstream 100 bases:

>100_bases
ATTTGCGCCGCTTTCGGGAAAGTGCTAAGCCGATTTATCGGCGGCAGGAGGTTTGCGGCCGTCATTCCCGTCGTCACGAT
GTCAGAGGCCTGAGAGCGCC

Downstream 100 bases:

>100_bases
TCGATCTGACGCCGACGAGCGAGGAAGAGGCGGCGGCGATTGTCCGCCACCATGCCGAAAGCGGCCGGCCGCTCGCTATC
TGCGGCGGCGATAGCCGCGC

Product: D-lactate dehydrogenase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSDAIPFLKPRADVLARRAQIVADLTDLLPPESLVHEARELVPFETDAFVSYRRLPLAVALPRSTAEVSAVMRYCHRYGI
PVVPRGAGTSLSGGAIPQEDAVVLGLSKMNHILEIDLPNRVAVVQAGVTNLNISESVSADGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVRMVLVDGTVIELGGKALDAAGYDLLGLVCGHEGQLGIVTEATVRLIAKPEGARPVLFG
FEASEEAGACVADVIAAGIIPVAIEFMDKPAIEICEAFAHAGYPLDVGALLIVEVEGSEAEMDASLKDIVEIARRHGVKT
VRECQSATEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLKRTAEIIDHYGLRVANVFHAGDGNMHPLILFNAN
DPEDAARAEAAGNDILKLCVDAGGCLTGEHGVGIEKRDLMRHQYSETDLAQQMAARAAFDPGWLLNPSKVFPLEGRPAA

Sequences:

>Translated_479_residues
MSDAIPFLKPRADVLARRAQIVADLTDLLPPESLVHEARELVPFETDAFVSYRRLPLAVALPRSTAEVSAVMRYCHRYGI
PVVPRGAGTSLSGGAIPQEDAVVLGLSKMNHILEIDLPNRVAVVQAGVTNLNISESVSADGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVRMVLVDGTVIELGGKALDAAGYDLLGLVCGHEGQLGIVTEATVRLIAKPEGARPVLFG
FEASEEAGACVADVIAAGIIPVAIEFMDKPAIEICEAFAHAGYPLDVGALLIVEVEGSEAEMDASLKDIVEIARRHGVKT
VRECQSATEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLKRTAEIIDHYGLRVANVFHAGDGNMHPLILFNAN
DPEDAARAEAAGNDILKLCVDAGGCLTGEHGVGIEKRDLMRHQYSETDLAQQMAARAAFDPGWLLNPSKVFPLEGRPAA
>Mature_478_residues
SDAIPFLKPRADVLARRAQIVADLTDLLPPESLVHEARELVPFETDAFVSYRRLPLAVALPRSTAEVSAVMRYCHRYGIP
VVPRGAGTSLSGGAIPQEDAVVLGLSKMNHILEIDLPNRVAVVQAGVTNLNISESVSADGFFYAPDPSSQLACTIGGNIG
MNSGGAHCLKYGVTTNNLLGVRMVLVDGTVIELGGKALDAAGYDLLGLVCGHEGQLGIVTEATVRLIAKPEGARPVLFGF
EASEEAGACVADVIAAGIIPVAIEFMDKPAIEICEAFAHAGYPLDVGALLIVEVEGSEAEMDASLKDIVEIARRHGVKTV
RECQSATEAALIWKGRKSAFGATGRIADYICMDGTVPLSQLSHVLKRTAEIIDHYGLRVANVFHAGDGNMHPLILFNAND
PEDAARAEAAGNDILKLCVDAGGCLTGEHGVGIEKRDLMRHQYSETDLAQQMAARAAFDPGWLLNPSKVFPLEGRPAA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=428, Percent_Identity=29.2056074766355, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=27.8026905829596, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI119964728, Length=449, Percent_Identity=28.5077951002227, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4501993, Length=237, Percent_Identity=27.0042194092827, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789351, Length=456, Percent_Identity=48.0263157894737, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787977, Length=508, Percent_Identity=25.9842519685039, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI48994907, Length=490, Percent_Identity=23.265306122449, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71992373, Length=430, Percent_Identity=27.4418604651163, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=430, Percent_Identity=27.4418604651163, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71992392, Length=365, Percent_Identity=27.6712328767123, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=487, Percent_Identity=26.0780287474333, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17556096, Length=465, Percent_Identity=22.3655913978495, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320027, Length=471, Percent_Identity=29.0870488322718, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320764, Length=492, Percent_Identity=26.6260162601626, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320023, Length=452, Percent_Identity=26.3274336283186, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18921117, Length=429, Percent_Identity=27.5058275058275, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24639277, Length=429, Percent_Identity=27.5058275058275, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24639275, Length=429, Percent_Identity=27.5058275058275, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24653753, Length=467, Percent_Identity=22.4839400428266, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50730; Mature: 50599

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAIPFLKPRADVLARRAQIVADLTDLLPPESLVHEARELVPFETDAFVSYRRLPLAVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCEEEE
LPRSTAEVSAVMRYCHRYGIPVVPRGAGTSLSGGAIPQEDAVVLGLSKMNHILEIDLPNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHCCCEEEEECCCC
VAVVQAGVTNLNISESVSADGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVRMVLVDGTVIELGGKALDAAGYDLLGLVCGHEGQLGIVTEATVRLIAKPEGARPVLFG
EEEEEEECCEEEECCCCEECCCCCCEEEEEECCCCCEEEEEHEEEEEEECCCCCCEEEEE
FEASEEAGACVADVIAAGIIPVAIEFMDKPAIEICEAFAHAGYPLDVGALLIVEVEGSEA
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EMDASLKDIVEIARRHGVKTVRECQSATEAALIWKGRKSAFGATGRIADYICMDGTVPLS
HHCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHEEEECCCCCHH
QLSHVLKRTAEIIDHYGLRVANVFHAGDGNMHPLILFNANDPEDAARAEAAGNDILKLCV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
DAGGCLTGEHGVGIEKRDLMRHQYSETDLAQQMAARAAFDPGWLLNPSKVFPLEGRPAA
CCCCCEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCCC
>Mature Secondary Structure 
SDAIPFLKPRADVLARRAQIVADLTDLLPPESLVHEARELVPFETDAFVSYRRLPLAVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCEEEE
LPRSTAEVSAVMRYCHRYGIPVVPRGAGTSLSGGAIPQEDAVVLGLSKMNHILEIDLPNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHCCCEEEEECCCC
VAVVQAGVTNLNISESVSADGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVRMVLVDGTVIELGGKALDAAGYDLLGLVCGHEGQLGIVTEATVRLIAKPEGARPVLFG
EEEEEEECCEEEECCCCEECCCCCCEEEEEECCCCCEEEEEHEEEEEEECCCCCCEEEEE
FEASEEAGACVADVIAAGIIPVAIEFMDKPAIEICEAFAHAGYPLDVGALLIVEVEGSEA
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EMDASLKDIVEIARRHGVKTVRECQSATEAALIWKGRKSAFGATGRIADYICMDGTVPLS
HHCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHEEEECCCCCHH
QLSHVLKRTAEIIDHYGLRVANVFHAGDGNMHPLILFNANDPEDAARAEAAGNDILKLCV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
DAGGCLTGEHGVGIEKRDLMRHQYSETDLAQQMAARAAFDPGWLLNPSKVFPLEGRPAA
CCCCCEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]