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The map label for this gene is hsrA [H]

Identifier: 209548037

GI number: 209548037

Start: 440375

End: 441799

Strand: Reverse

Name: hsrA [H]

Synonym: Rleg2_0430

Alternate gene names: 209548037

Gene position: 441799-440375 (Counterclockwise)

Preceding gene: 209548041

Following gene: 209548010

Centisome position: 9.74

GC content: 64.28

Gene sequence:

>1425_bases
ATGAACCGCATCGTCCCACTGATCCTTGCCGTCGCTCTTTTCATGGAACAGATGGACTCCACCGTCATTGCCACGGCGCT
GCCGGCGATCGCCGCGGATCTCAATGTCGGGCCGATCACGCTGAAACTGGCGCTGACCTCCTATATGGTGGCGCTGGCGG
TGTTCATCCCGGTCAGCGGCTGGATGGCCGACAGGTTCGGCGCCAAGAAGATCTTCCGCCTTGCCATCTCGGTCTTCGTC
ATCGGCTCGATCTTCTGCGCCGTCTCGTCCAACCTCGTCGAGTTCGTGTTCGCCCGCTTCCTGCAGGGCATGGGTGGGGC
GATGATGACGCCGGTCGGCCGTCTGGTGCTGGTGCGCACGACGAAGCGCAGCGATCTGGTCTCGGCCATGGCGCTGCTCA
CCATCCCGGCGCTGGTCGGCCCGCTCACCGGCCCGCCGCTCGGCGGCTTCATCACCACCTATTTCAGCTGGCACTGGATC
TTCCTGATCAACGTGCCGGTCGGCATCATCGGCATCTGGCTGGCGACGATCTTCCTGCCGGAGGTCGAGGCGACGGCGCC
GCCGAAGCTTGATTTCACCGGTTTCGTGCTCACCTCGCTTTCGGCCGCGGGCGTCGTCTTCGGCCTGTCGGTGGTCAGCC
TGCCGGCTCTGCCGCCGATCATAGGCGTCACCGCCACCGCGATCGGCCTCATCTGCGGCTTCCTCTATATCCGCCATGCC
AGGCGCCACCCGACGCCGATCCTCAACCTCGACCTGTTCAAGAACCCGACCTTCCGGGCCTCGACGCTGGGCGGCACGCT
CTTTCGCATCTGCGTCGGCGCCATGCCCTTCCTGACGCCGCTGATGCTGCAGCTGGGCTTCGGGCTGACGCCGTTTCAAT
CCGGCCTGATCACCTTTGCCGGCGCCATCGGGGCGATCACCACCAAATTCATGGCAAAACGCGTCTTTGCCGCTGCCGGC
TTCCGCACGACGCTGCTTTGCGCCGCCATGGTGACGACCCTCGTGACCATCGTCACCGGCCTGTTCACGCCGGCAACGCC
GCATCTGGTCATCATCGGCGTGCTGCTCATCGGCGGCTTCTCCCGCTCCTTCATGTTCACCGGCGTCAATGCGCTTGCCT
TCGCCGATATCGACGATGCCGAGGCGAGCCAAGCGACATCGATGAGCTCGGTGATGCAGCAGGTCAGCCTGGCGCTCGGT
GTCGCGGTCGCCGCGGCGATCCTGGAAAGCTCGATCTATATCAGGGGCGAAGCGCTGCAGGTCGCCGATTTCCACCTTGC
CTTCTATATCATCGCCGGTCTGACCGTCATCGCCACCATTCCCTTCGTCAGAATGGACCGCAATGCCGGCGCACTCGTTT
CCGGCCACCGCGCGAAGGTGGTGACGGTATCGACCGTTGAAGCCGAGCAGCCGGTGGTGAAATAG

Upstream 100 bases:

>100_bases
ATGGCATCGAACTGGATTTGAGCATTGGCAGGCCCCTGAAGTGGGACTAAACAGCACGGGCCTTCCCGCTTCCATCCGCG
CCCATCAAGGCCTCCCGCCT

Downstream 100 bases:

>100_bases
GCTCAAGGTGCTGCAGCGTGCTTTGCGCGCCTCAAAGCGCGGCGCTCGTTCTATTCCGGGTTTTCGCAGACGGCAGAGAG
CTTGTTGCCGTCGAGATCGC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNRIVPLILAVALFMEQMDSTVIATALPAIAADLNVGPITLKLALTSYMVALAVFIPVSGWMADRFGAKKIFRLAISVFV
IGSIFCAVSSNLVEFVFARFLQGMGGAMMTPVGRLVLVRTTKRSDLVSAMALLTIPALVGPLTGPPLGGFITTYFSWHWI
FLINVPVGIIGIWLATIFLPEVEATAPPKLDFTGFVLTSLSAAGVVFGLSVVSLPALPPIIGVTATAIGLICGFLYIRHA
RRHPTPILNLDLFKNPTFRASTLGGTLFRICVGAMPFLTPLMLQLGFGLTPFQSGLITFAGAIGAITTKFMAKRVFAAAG
FRTTLLCAAMVTTLVTIVTGLFTPATPHLVIIGVLLIGGFSRSFMFTGVNALAFADIDDAEASQATSMSSVMQQVSLALG
VAVAAAILESSIYIRGEALQVADFHLAFYIIAGLTVIATIPFVRMDRNAGALVSGHRAKVVTVSTVEAEQPVVK

Sequences:

>Translated_474_residues
MNRIVPLILAVALFMEQMDSTVIATALPAIAADLNVGPITLKLALTSYMVALAVFIPVSGWMADRFGAKKIFRLAISVFV
IGSIFCAVSSNLVEFVFARFLQGMGGAMMTPVGRLVLVRTTKRSDLVSAMALLTIPALVGPLTGPPLGGFITTYFSWHWI
FLINVPVGIIGIWLATIFLPEVEATAPPKLDFTGFVLTSLSAAGVVFGLSVVSLPALPPIIGVTATAIGLICGFLYIRHA
RRHPTPILNLDLFKNPTFRASTLGGTLFRICVGAMPFLTPLMLQLGFGLTPFQSGLITFAGAIGAITTKFMAKRVFAAAG
FRTTLLCAAMVTTLVTIVTGLFTPATPHLVIIGVLLIGGFSRSFMFTGVNALAFADIDDAEASQATSMSSVMQQVSLALG
VAVAAAILESSIYIRGEALQVADFHLAFYIIAGLTVIATIPFVRMDRNAGALVSGHRAKVVTVSTVEAEQPVVK
>Mature_474_residues
MNRIVPLILAVALFMEQMDSTVIATALPAIAADLNVGPITLKLALTSYMVALAVFIPVSGWMADRFGAKKIFRLAISVFV
IGSIFCAVSSNLVEFVFARFLQGMGGAMMTPVGRLVLVRTTKRSDLVSAMALLTIPALVGPLTGPPLGGFITTYFSWHWI
FLINVPVGIIGIWLATIFLPEVEATAPPKLDFTGFVLTSLSAAGVVFGLSVVSLPALPPIIGVTATAIGLICGFLYIRHA
RRHPTPILNLDLFKNPTFRASTLGGTLFRICVGAMPFLTPLMLQLGFGLTPFQSGLITFAGAIGAITTKFMAKRVFAAAG
FRTTLLCAAMVTTLVTIVTGLFTPATPHLVIIGVLLIGGFSRSFMFTGVNALAFADIDDAEASQATSMSSVMQQVSLALG
VAVAAAILESSIYIRGEALQVADFHLAFYIIAGLTVIATIPFVRMDRNAGALVSGHRAKVVTVSTVEAEQPVVK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=472, Percent_Identity=40.8898305084746, Blast_Score=321, Evalue=6e-89,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=37.4064837905237, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788710, Length=433, Percent_Identity=28.4064665127021, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081983, Length=297, Percent_Identity=28.956228956229, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789042, Length=304, Percent_Identity=30.2631578947368, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790146, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=30.7086614173228, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50085; Mature: 50085

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIVPLILAVALFMEQMDSTVIATALPAIAADLNVGPITLKLALTSYMVALAVFIPVSG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCC
WMADRFGAKKIFRLAISVFVIGSIFCAVSSNLVEFVFARFLQGMGGAMMTPVGRLVLVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEE
TKRSDLVSAMALLTIPALVGPLTGPPLGGFITTYFSWHWIFLINVPVGIIGIWLATIFLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHC
EVEATAPPKLDFTGFVLTSLSAAGVVFGLSVVSLPALPPIIGVTATAIGLICGFLYIRHA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RRHPTPILNLDLFKNPTFRASTLGGTLFRICVGAMPFLTPLMLQLGFGLTPFQSGLITFA
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GAIGAITTKFMAKRVFAAAGFRTTLLCAAMVTTLVTIVTGLFTPATPHLVIIGVLLIGGF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
SRSFMFTGVNALAFADIDDAEASQATSMSSVMQQVSLALGVAVAAAILESSIYIRGEALQ
CHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEE
VADFHLAFYIIAGLTVIATIPFVRMDRNAGALVSGHRAKVVTVSTVEAEQPVVK
HHHHHHHHHHHHHHHHHHHCCHHEECCCCCCEEECCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MNRIVPLILAVALFMEQMDSTVIATALPAIAADLNVGPITLKLALTSYMVALAVFIPVSG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCC
WMADRFGAKKIFRLAISVFVIGSIFCAVSSNLVEFVFARFLQGMGGAMMTPVGRLVLVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEE
TKRSDLVSAMALLTIPALVGPLTGPPLGGFITTYFSWHWIFLINVPVGIIGIWLATIFLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHC
EVEATAPPKLDFTGFVLTSLSAAGVVFGLSVVSLPALPPIIGVTATAIGLICGFLYIRHA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RRHPTPILNLDLFKNPTFRASTLGGTLFRICVGAMPFLTPLMLQLGFGLTPFQSGLITFA
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GAIGAITTKFMAKRVFAAAGFRTTLLCAAMVTTLVTIVTGLFTPATPHLVIIGVLLIGGF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
SRSFMFTGVNALAFADIDDAEASQATSMSSVMQQVSLALGVAVAAAILESSIYIRGEALQ
CHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEE
VADFHLAFYIIAGLTVIATIPFVRMDRNAGALVSGHRAKVVTVSTVEAEQPVVK
HHHHHHHHHHHHHHHHHHHCCHHEECCCCCCEEECCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]