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The map label for this gene is strE [H]

Identifier: 209548016

GI number: 209548016

Start: 420213

End: 421193

Strand: Direct

Name: strE [H]

Synonym: Rleg2_0408

Alternate gene names: 209548016

Gene position: 420213-421193 (Clockwise)

Preceding gene: 209548015

Following gene: 209548017

Centisome position: 9.26

GC content: 57.39

Gene sequence:

>981_bases
ATGAAGAAAGCGCTTGTCACCGGGGCAGATGGGTTCATCGGTTCTCATCTCGTCGAAACGCTGGTCAGGTCGGGTGTCGA
GGTTCGCGCCCTCTGCCAGTATAACTCGTTTTCCAGTTGGGGCTGGCTCGACCAATCGGAATATCGCGGCAAGTTCGAGG
TGATCCTCGGAGACGTCCGTGATCCCGCCCAGATGCGCTCCGTCGCCAGAGATGTCGACACGATCTTCCATCTTGCCGCT
CTGATTGCGATTCCCTATTCCTATCAGGCTCCCTCGAGCTACATCGATACCAATGTGCATGGGACGCTGAACGTTCTTCA
AGGCGCTCTCGACGCCGGCGTCGGAAGAGTGATCCAGACCTCGACGAGCGAAGTCTATGGGACGGCACGTTTTGTGCCCA
TCAGCGAAAGCCATCCTTTGCAGGCGCAGTCGCCCTATTCGGCGTCCAAAATCGGTGCCGATGCGATCGCCTACAGCTAT
CACTCGAGCTTCGATCTGCCGGTGACGATCGCACGGCCGTTCAACACCTACGGCCCGAGACAATCTGCAAGGGCGGTTAT
TCCAACCGTGATTTCGCAGCTTCTGAGTGGACGAAGGACGCTCAAGCTTGGTGCGCTCTCGCCCACCCGGGATTTCAATT
TCGTGCAGGATACATGCGACGGCTTTCTGGCGCTCGCGGCCTGCGACAAAGCCATCGGACAGACGGTCAATATCGGCTCG
GGCGGCGAGATATCGATCGGCGATACCGTTCGGCTGATCGCCGATATCATCGGCGTCAACATCGAGATCGAATGCGACGA
ACAGCGTTTGCGTCCGGCAAACAGCGAAGTGGAACGCCTGTGCTGTGATAACAGCCTGATCAAGTCTCTGACGGGATTCT
CGCCTCGCTACAGCTTGAAAGACGGGCTCCAGGCGACGATTGAATGGCTGCGTCAGCCCGAGAATCTGGCGCGGTATAAG
GCGGATATTTTCAATGTCTAG

Upstream 100 bases:

>100_bases
TCCAGATGCTGGGTCAGCAACGGAAGCCATCGATTTCGGCGATACAGCTGCCTTGGGAGGTTGAGAAGCTCTTCCTGCGC
GATTCTGAGGGTGTGTTGAA

Downstream 100 bases:

>100_bases
GCGAGCAGTCATTCTGGCAGGGGGAATGGGAACGCGGCTGCGCCCTTATACCGTCGTGCTTCCCAAGCCGCTGATGCCGA
TCGGCGATTATCCTATTCTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKKALVTGADGFIGSHLVETLVRSGVEVRALCQYNSFSSWGWLDQSEYRGKFEVILGDVRDPAQMRSVARDVDTIFHLAA
LIAIPYSYQAPSSYIDTNVHGTLNVLQGALDAGVGRVIQTSTSEVYGTARFVPISESHPLQAQSPYSASKIGADAIAYSY
HSSFDLPVTIARPFNTYGPRQSARAVIPTVISQLLSGRRTLKLGALSPTRDFNFVQDTCDGFLALAACDKAIGQTVNIGS
GGEISIGDTVRLIADIIGVNIEIECDEQRLRPANSEVERLCCDNSLIKSLTGFSPRYSLKDGLQATIEWLRQPENLARYK
ADIFNV

Sequences:

>Translated_326_residues
MKKALVTGADGFIGSHLVETLVRSGVEVRALCQYNSFSSWGWLDQSEYRGKFEVILGDVRDPAQMRSVARDVDTIFHLAA
LIAIPYSYQAPSSYIDTNVHGTLNVLQGALDAGVGRVIQTSTSEVYGTARFVPISESHPLQAQSPYSASKIGADAIAYSY
HSSFDLPVTIARPFNTYGPRQSARAVIPTVISQLLSGRRTLKLGALSPTRDFNFVQDTCDGFLALAACDKAIGQTVNIGS
GGEISIGDTVRLIADIIGVNIEIECDEQRLRPANSEVERLCCDNSLIKSLTGFSPRYSLKDGLQATIEWLRQPENLARYK
ADIFNV
>Mature_326_residues
MKKALVTGADGFIGSHLVETLVRSGVEVRALCQYNSFSSWGWLDQSEYRGKFEVILGDVRDPAQMRSVARDVDTIFHLAA
LIAIPYSYQAPSSYIDTNVHGTLNVLQGALDAGVGRVIQTSTSEVYGTARFVPISESHPLQAQSPYSASKIGADAIAYSY
HSSFDLPVTIARPFNTYGPRQSARAVIPTVISQLLSGRRTLKLGALSPTRDFNFVQDTCDGFLALAACDKAIGQTVNIGS
GGEISIGDTVRLIADIIGVNIEIECDEQRLRPANSEVERLCCDNSLIKSLTGFSPRYSLKDGLQATIEWLRQPENLARYK
ADIFNV

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=31.7073170731707, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=31.4641744548287, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=24.4252873563218, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI56237023, Length=345, Percent_Identity=25.2173913043478, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI56118217, Length=345, Percent_Identity=25.2173913043478, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI189083684, Length=345, Percent_Identity=25.2173913043478, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI193211614, Length=229, Percent_Identity=26.2008733624454, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI8393516, Length=229, Percent_Identity=26.2008733624454, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=28.8951841359773, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=30.3834808259587, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788366, Length=343, Percent_Identity=25.3644314868805, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786974, Length=294, Percent_Identity=29.5918367346939, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=30.8868501529052, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17568069, Length=320, Percent_Identity=25.9375, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=24.6951219512195, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=24.7813411078717, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=279, Percent_Identity=24.0143369175627, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=23.1671554252199, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=280, Percent_Identity=24.6428571428571, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=280, Percent_Identity=24.6428571428571, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=282, Percent_Identity=24.468085106383, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=26.3456090651558, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.0744985673352, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=303, Percent_Identity=26.4026402640264, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=31.5315315315315, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19923002, Length=346, Percent_Identity=27.4566473988439, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24158427, Length=347, Percent_Identity=23.6311239193084, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 35516; Mature: 35516

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALVTGADGFIGSHLVETLVRSGVEVRALCQYNSFSSWGWLDQSEYRGKFEVILGDVR
CCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCEEEEEEECCC
DPAQMRSVARDVDTIFHLAALIAIPYSYQAPSSYIDTNVHGTLNVLQGALDAGVGRVIQT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
STSEVYGTARFVPISESHPLQAQSPYSASKIGADAIAYSYHSSFDLPVTIARPFNTYGPR
CCHHHCCCEEEEECCCCCCCCCCCCCCHHHHCHHHHEEECCCCCCCCEEEECCCCCCCCC
QSARAVIPTVISQLLSGRRTLKLGALSPTRDFNFVQDTCDGFLALAACDKAIGQTVNIGS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCC
GGEISIGDTVRLIADIIGVNIEIECDEQRLRPANSEVERLCCDNSLIKSLTGFSPRYSLK
CCCEECCHHHHHHHHHHCCCEEEEECHHHCCCCCHHHHHHHHCHHHHHHHCCCCCCCCHH
DGLQATIEWLRQPENLARYKADIFNV
HHHHHHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKALVTGADGFIGSHLVETLVRSGVEVRALCQYNSFSSWGWLDQSEYRGKFEVILGDVR
CCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCEEEEEEECCC
DPAQMRSVARDVDTIFHLAALIAIPYSYQAPSSYIDTNVHGTLNVLQGALDAGVGRVIQT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
STSEVYGTARFVPISESHPLQAQSPYSASKIGADAIAYSYHSSFDLPVTIARPFNTYGPR
CCHHHCCCEEEEECCCCCCCCCCCCCCHHHHCHHHHEEECCCCCCCCEEEECCCCCCCCC
QSARAVIPTVISQLLSGRRTLKLGALSPTRDFNFVQDTCDGFLALAACDKAIGQTVNIGS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCC
GGEISIGDTVRLIADIIGVNIEIECDEQRLRPANSEVERLCCDNSLIKSLTGFSPRYSLK
CCCEECCHHHHHHHHHHCCCEEEEECHHHCCCCCHHHHHHHHCHHHHHHHCCCCCCCCHH
DGLQATIEWLRQPENLARYKADIFNV
HHHHHHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661369 [H]