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The map label for this gene is gutB [H]

Identifier: 209547878

GI number: 209547878

Start: 274394

End: 275404

Strand: Direct

Name: gutB [H]

Synonym: Rleg2_0270

Alternate gene names: 209547878

Gene position: 274394-275404 (Clockwise)

Preceding gene: 209547877

Following gene: 209547880

Centisome position: 6.05

GC content: 64.49

Gene sequence:

>1011_bases
ATGAGAGCTGTACGCTTGGAGTCGATCGGGTCTTTGACCATGCGCAGCGTTGAGAGACCGGTTGCCGGGCGGGGCGAGCT
GCTTGTCCGGGTCGCGGCCGCCGGCATCTGCGGTTCCGATCGCCACATGTACAAGGGCGAATATCCGACAGCGATCCCCG
TCACGCTGGGCCACGAATTCTGCGGCATCGTCGAAGAGATCGGCGATGGCGTCACCCGCTTTGCCGGCGGCGAGTTGGTG
ACGGTCGACCCGAATATCGCCTGCGGCACCTGTCCGGCTTGCACACGGGCGCGGCCGAACCTATGCGAGAGACTGACCGC
CATCGGCGTGACGCGGGATGGTGGCTTTGCCGAATATGTGGCGGTGCCGCATGCGCAGGCCTTCATCCTGCCGGTCGGCC
TCGATCCCGTTCACGGCGCCTTTTCCGAACCGTTGGCCTGTTGCATCCACGCCATCGACAAGGCAGGAATCCGCCCTGGC
GACAACGTCGCCATCCTCGGCGGGGGCGTGATCGGCCTGCTGATGGTGCAGCTGGCTCGTCTGGCGGGGGCAGGCGAGAT
CATTTTGATCACGCGGCAGCAATCGAGACGTCAGGCCGCTCTGCGCCTGGGGGTGACGCACGCCTTCGACCCGAATTCTT
CGGACACGGTCGCGTCCGTTCGAGAGGTCACCAAGGGCGGCGCCGATGTGGTCATCGAGTGCGCCGGCGTCAGTGACACG
CTTCAAAGCGGTCTGAAGATGGCGCGGCGCGGTGGCGCCTTCGTGCTTTTCGGGGTCACGCCTGCGGGTGTCGAGGTGCC
GGTTCTGCCTTTCGATCTGCTGATCAACGAAGTCGATATCAGGCCGGCCTACCTCAACCCCTTCACCCATTCGCGCGCTG
CGGCAATGGTCGCAAGCGGAGCATTGGAACTGGACGCATTGGTCACCAGAACCATCGGTCTCGAAGAGGTCGCCGACGTG
GTGGTCAACGCGCCATTGCCAGGCGAGATCAAGGTCATCGTCCGGCCTTAG

Upstream 100 bases:

>100_bases
TGGCGCAGTGCTACAATGTCGATGGCGGCAATGTCATGAGCTGAGCGCTTCGCTTCATCGCCAGGAATAGGCAATCGAGC
TTGAGGTCTGGAGGAGTGGA

Downstream 100 bases:

>100_bases
AGCATGGTGCTGCGGACGGGGCCGGTGGAATCGCGCGTAATCAGCGTGACCGGCACTTTGACAATCGCAGCCGCTCGAGA
TCCGTCTCCTGTTTGCTGGT

Product: alcohol dehydrogenase GroES domain-containing protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRAVRLESIGSLTMRSVERPVAGRGELLVRVAAAGICGSDRHMYKGEYPTAIPVTLGHEFCGIVEEIGDGVTRFAGGELV
TVDPNIACGTCPACTRARPNLCERLTAIGVTRDGGFAEYVAVPHAQAFILPVGLDPVHGAFSEPLACCIHAIDKAGIRPG
DNVAILGGGVIGLLMVQLARLAGAGEIILITRQQSRRQAALRLGVTHAFDPNSSDTVASVREVTKGGADVVIECAGVSDT
LQSGLKMARRGGAFVLFGVTPAGVEVPVLPFDLLINEVDIRPAYLNPFTHSRAAAMVASGALELDALVTRTIGLEEVADV
VVNAPLPGEIKVIVRP

Sequences:

>Translated_336_residues
MRAVRLESIGSLTMRSVERPVAGRGELLVRVAAAGICGSDRHMYKGEYPTAIPVTLGHEFCGIVEEIGDGVTRFAGGELV
TVDPNIACGTCPACTRARPNLCERLTAIGVTRDGGFAEYVAVPHAQAFILPVGLDPVHGAFSEPLACCIHAIDKAGIRPG
DNVAILGGGVIGLLMVQLARLAGAGEIILITRQQSRRQAALRLGVTHAFDPNSSDTVASVREVTKGGADVVIECAGVSDT
LQSGLKMARRGGAFVLFGVTPAGVEVPVLPFDLLINEVDIRPAYLNPFTHSRAAAMVASGALELDALVTRTIGLEEVADV
VVNAPLPGEIKVIVRP
>Mature_336_residues
MRAVRLESIGSLTMRSVERPVAGRGELLVRVAAAGICGSDRHMYKGEYPTAIPVTLGHEFCGIVEEIGDGVTRFAGGELV
TVDPNIACGTCPACTRARPNLCERLTAIGVTRDGGFAEYVAVPHAQAFILPVGLDPVHGAFSEPLACCIHAIDKAGIRPG
DNVAILGGGVIGLLMVQLARLAGAGEIILITRQQSRRQAALRLGVTHAFDPNSSDTVASVREVTKGGADVVIECAGVSDT
LQSGLKMARRGGAFVLFGVTPAGVEVPVLPFDLLINEVDIRPAYLNPFTHSRAAAMVASGALELDALVTRTIGLEEVADV
VVNAPLPGEIKVIVRP

Specific function: Reduces glucitol to fructose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=315, Percent_Identity=28.8888888888889, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4501929, Length=357, Percent_Identity=26.0504201680672, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4501933, Length=357, Percent_Identity=26.3305322128852, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI34577061, Length=357, Percent_Identity=26.0504201680672, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI71565154, Length=355, Percent_Identity=24.7887323943662, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4501939, Length=338, Percent_Identity=25.4437869822485, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI156523966, Length=338, Percent_Identity=25.4437869822485, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI71565152, Length=372, Percent_Identity=25.2688172043011, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI71743840, Length=364, Percent_Identity=24.4505494505494, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI262073058, Length=364, Percent_Identity=24.4505494505494, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI18379349, Length=244, Percent_Identity=26.2295081967213, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI226510992, Length=262, Percent_Identity=35.8778625954198, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1788073, Length=320, Percent_Identity=30, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787863, Length=323, Percent_Identity=29.4117647058824, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788407, Length=337, Percent_Identity=30.2670623145401, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790045, Length=327, Percent_Identity=27.217125382263, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788075, Length=333, Percent_Identity=28.2282282282282, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082125, Length=333, Percent_Identity=29.4294294294294, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790718, Length=285, Percent_Identity=30.1754385964912, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786552, Length=352, Percent_Identity=30.3977272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081918, Length=267, Percent_Identity=30.3370786516854, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786518, Length=293, Percent_Identity=23.2081911262799, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=339, Percent_Identity=29.4985250737463, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17562878, Length=335, Percent_Identity=29.8507462686567, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25146526, Length=313, Percent_Identity=27.1565495207668, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71997431, Length=305, Percent_Identity=27.5409836065574, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562584, Length=280, Percent_Identity=26.7857142857143, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=330, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17565904, Length=288, Percent_Identity=27.4305555555556, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=281, Percent_Identity=25.9786476868327, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322619, Length=333, Percent_Identity=30.6306306306306, Blast_Score=132, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319955, Length=333, Percent_Identity=30.3303303303303, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323099, Length=334, Percent_Identity=29.6407185628743, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319258, Length=316, Percent_Identity=30.6962025316456, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320033, Length=335, Percent_Identity=27.7611940298507, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323961, Length=300, Percent_Identity=28, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319257, Length=384, Percent_Identity=26.0416666666667, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=326, Percent_Identity=26.6871165644172, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=300, Percent_Identity=27.3333333333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137530, Length=335, Percent_Identity=31.044776119403, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=30.791788856305, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17737895, Length=321, Percent_Identity=28.0373831775701, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI221457811, Length=317, Percent_Identity=22.397476340694, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550770, Length=317, Percent_Identity=22.397476340694, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45551930, Length=317, Percent_Identity=22.397476340694, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 35080; Mature: 35080

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVRLESIGSLTMRSVERPVAGRGELLVRVAAAGICGSDRHMYKGEYPTAIPVTLGHEF
CCEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCCCCEEEEECCHHH
CGIVEEIGDGVTRFAGGELVTVDPNIACGTCPACTRARPNLCERLTAIGVTRDGGFAEYV
HHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEE
AVPHAQAFILPVGLDPVHGAFSEPLACCIHAIDKAGIRPGDNVAILGGGVIGLLMVQLAR
ECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHH
LAGAGEIILITRQQSRRQAALRLGVTHAFDPNSSDTVASVREVTKGGADVVIECAGVSDT
HCCCCCEEEEECCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHH
LQSGLKMARRGGAFVLFGVTPAGVEVPVLPFDLLINEVDIRPAYLNPFTHSRAAAMVASG
HHHHHHHHHCCCCEEEEECCCCCCEECCEEHHHHHHHCCCCCEEECCCCHHHHHHHHHCC
ALELDALVTRTIGLEEVADVVVNAPLPGEIKVIVRP
CHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRAVRLESIGSLTMRSVERPVAGRGELLVRVAAAGICGSDRHMYKGEYPTAIPVTLGHEF
CCEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCCCCEEEEECCHHH
CGIVEEIGDGVTRFAGGELVTVDPNIACGTCPACTRARPNLCERLTAIGVTRDGGFAEYV
HHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEE
AVPHAQAFILPVGLDPVHGAFSEPLACCIHAIDKAGIRPGDNVAILGGGVIGLLMVQLAR
ECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHH
LAGAGEIILITRQQSRRQAALRLGVTHAFDPNSSDTVASVREVTKGGADVVIECAGVSDT
HCCCCCEEEEECCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHH
LQSGLKMARRGGAFVLFGVTPAGVEVPVLPFDLLINEVDIRPAYLNPFTHSRAAAMVASG
HHHHHHHHHCCCCEEEEECCCCCCEECCEEHHHHHHHCCCCCEEECCCCHHHHHHHHHCC
ALELDALVTRTIGLEEVADVVVNAPLPGEIKVIVRP
CHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]