| Definition | Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome. |
|---|---|
| Accession | NC_011369 |
| Length | 4,537,948 |
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The map label for this gene is polS [H]
Identifier: 209547877
GI number: 209547877
Start: 273546
End: 274337
Strand: Direct
Name: polS [H]
Synonym: Rleg2_0269
Alternate gene names: 209547877
Gene position: 273546-274337 (Clockwise)
Preceding gene: 209547876
Following gene: 209547878
Centisome position: 6.03
GC content: 63.64
Gene sequence:
>792_bases ATGACAGATACGGAAACGGGGCGGCGGCTCTCCGGCAAGGTGGCATTGGTCACGGGAGGAGCAAGCGGCATCGGCAAGGC GGTATGCCAACGCTTCGCCGCCGAAGGCGCCAAGGTTGTCGTCGCCGACCTCGACGCTGGCAAATGCGTGGCAGTGGCGG AAGCGATCGGCGCGGATGCCTGGGGTGTCGCGCTCGATGTGACCCGCCAGGACAGCATCGAAGACGCCGTCCGCTTTTCC ATCGCCGCCGCCGGCAAGATCGACATTCTGGTCAACGCCGCAGGCATTTACGAGGTCGAGTCGATTCTGGAAATATCCCG GGAGCGGACGGCGAAGGTGTTTCAGGTCAATATCGAAGGCCTGATCTTCATGACACAGGTCGTTGCCAGGCACATGGTGG AGAGAGGGGAAGGTGGACGCATCATCAACTTCTCCTCCCAGGCCGGGCGCCGGGGCGAAGGACCGGCGGTGGCTTACTGC GCTTCCAAGGCGGCGGTCATCAGCATCACGCAAAGCTGCGCCCTGGAGCTGATCCGCTACGGGATCAACGTCAACGCCAT CGCTCCCGGCGTCGTCGATACGCCGATGTGGGATATCGTCGATGCAAAACTCGGCAGCCGGGAAGGGTTGCAACCCGGCG AGGTGAAGGCCCGCGTGGCCGCTGCCGTCCCGGCCGGTCGATTTGGCGCCGCTGAAGAACAAGCCGCCATGGCGGCCTTC CTGGCCGGACCCGATGCAGCATACATCGTGGCGCAGTGCTACAATGTCGATGGCGGCAATGTCATGAGCTGA
Upstream 100 bases:
>100_bases ATGCCAGGCAGTCAAAAAGGGAGGAGCACATGAATCTATTCACCATGGCAGCCGCGCACGGCCGCTCTGCCGGTGCAGAT GCGGCCGGGGAGGCAGCGCG
Downstream 100 bases:
>100_bases GCGCTTCGCTTCATCGCCAGGAATAGGCAATCGAGCTTGAGGTCTGGAGGAGTGGAATGAGAGCTGTACGCTTGGAGTCG ATCGGGTCTTTGACCATGCG
Product: sorbitol dehydrogenase
Products: NA
Alternate protein names: L-iditol 2-dehydrogenase; Polyol dehydrogenase [H]
Number of amino acids: Translated: 263; Mature: 262
Protein sequence:
>263_residues MTDTETGRRLSGKVALVTGGASGIGKAVCQRFAAEGAKVVVADLDAGKCVAVAEAIGADAWGVALDVTRQDSIEDAVRFS IAAAGKIDILVNAAGIYEVESILEISRERTAKVFQVNIEGLIFMTQVVARHMVERGEGGRIINFSSQAGRRGEGPAVAYC ASKAAVISITQSCALELIRYGINVNAIAPGVVDTPMWDIVDAKLGSREGLQPGEVKARVAAAVPAGRFGAAEEQAAMAAF LAGPDAAYIVAQCYNVDGGNVMS
Sequences:
>Translated_263_residues MTDTETGRRLSGKVALVTGGASGIGKAVCQRFAAEGAKVVVADLDAGKCVAVAEAIGADAWGVALDVTRQDSIEDAVRFS IAAAGKIDILVNAAGIYEVESILEISRERTAKVFQVNIEGLIFMTQVVARHMVERGEGGRIINFSSQAGRRGEGPAVAYC ASKAAVISITQSCALELIRYGINVNAIAPGVVDTPMWDIVDAKLGSREGLQPGEVKARVAAAVPAGRFGAAEEQAAMAAF LAGPDAAYIVAQCYNVDGGNVMS >Mature_262_residues TDTETGRRLSGKVALVTGGASGIGKAVCQRFAAEGAKVVVADLDAGKCVAVAEAIGADAWGVALDVTRQDSIEDAVRFSI AAAGKIDILVNAAGIYEVESILEISRERTAKVFQVNIEGLIFMTQVVARHMVERGEGGRIINFSSQAGRRGEGPAVAYCA SKAAVISITQSCALELIRYGINVNAIAPGVVDTPMWDIVDAKLGSREGLQPGEVKARVAAAVPAGRFGAAEEQAAMAAFL AGPDAAYIVAQCYNVDGGNVMS
Specific function: Catalyzes the oxidation of D-glucitol (sorbitol) to D- fructose, galactitol to D-tagatose and of L-iditol [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI15277342, Length=270, Percent_Identity=33.7037037037037, Blast_Score=126, Evalue=2e-29, Organism=Homo sapiens, GI7705925, Length=255, Percent_Identity=32.156862745098, Blast_Score=112, Evalue=5e-25, Organism=Homo sapiens, GI40254992, Length=249, Percent_Identity=30.1204819277108, Blast_Score=101, Evalue=7e-22, Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=31.7460317460317, Blast_Score=101, Evalue=7e-22, Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=30.9803921568627, Blast_Score=101, Evalue=8e-22, Organism=Homo sapiens, GI19923817, Length=262, Percent_Identity=29.0076335877863, Blast_Score=100, Evalue=1e-21, Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=34.8039215686275, Blast_Score=98, Evalue=6e-21, Organism=Homo sapiens, GI59889578, Length=217, Percent_Identity=31.3364055299539, Blast_Score=98, Evalue=7e-21, Organism=Homo sapiens, GI4758504, Length=208, Percent_Identity=32.2115384615385, Blast_Score=95, Evalue=5e-20, Organism=Homo sapiens, GI5031737, Length=258, Percent_Identity=28.6821705426357, Blast_Score=86, Evalue=2e-17, Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=4e-17, Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=28.2945736434109, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI4504505, Length=193, Percent_Identity=34.1968911917098, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=35.2601156069364, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI83715985, Length=186, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI7706318, Length=210, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI4503301, Length=255, Percent_Identity=27.4509803921569, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI142976729, Length=226, Percent_Identity=25.2212389380531, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI210032110, Length=193, Percent_Identity=26.4248704663212, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI33667109, Length=209, Percent_Identity=31.1004784688995, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI126723191, Length=193, Percent_Identity=30.5699481865285, Blast_Score=74, Evalue=2e-13, Organism=Homo sapiens, GI20149619, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=4e-13, Organism=Homo sapiens, GI4503817, Length=194, Percent_Identity=26.8041237113402, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI40807363, Length=198, Percent_Identity=24.7474747474747, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI7705855, Length=193, Percent_Identity=31.6062176165803, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI225579078, Length=210, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI109715829, Length=196, Percent_Identity=30.6122448979592, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI226371731, Length=191, Percent_Identity=29.8429319371728, Blast_Score=65, Evalue=7e-11, Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=35.5731225296443, Blast_Score=144, Evalue=5e-36, Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=32.03125, Blast_Score=129, Evalue=2e-31, Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=33.7209302325581, Blast_Score=126, Evalue=1e-30, Organism=Escherichia coli, GI1788459, Length=257, Percent_Identity=33.4630350194552, Blast_Score=126, Evalue=1e-30, Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=32.5396825396825, Blast_Score=123, Evalue=1e-29, Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=31.6205533596838, Blast_Score=117, Evalue=9e-28, Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=31.3492063492063, Blast_Score=108, Evalue=5e-25, Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=31.25, Blast_Score=101, Evalue=5e-23, Organism=Escherichia coli, GI2367365, Length=260, Percent_Identity=31.9230769230769, Blast_Score=100, Evalue=8e-23, Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=31.496062992126, Blast_Score=91, Evalue=5e-20, Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=28.4615384615385, Blast_Score=90, Evalue=2e-19, Organism=Escherichia coli, GI1786812, Length=260, Percent_Identity=31.1538461538462, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=31.6666666666667, Blast_Score=79, Evalue=4e-16, Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=36.328125, Blast_Score=140, Evalue=4e-34, Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=35.4581673306773, Blast_Score=138, Evalue=3e-33, Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=34.8314606741573, Blast_Score=122, Evalue=2e-28, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=32.295719844358, Blast_Score=117, Evalue=6e-27, Organism=Caenorhabditis elegans, GI193204405, Length=272, Percent_Identity=31.6176470588235, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=30.8, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=31.9548872180451, Blast_Score=108, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=30.4511278195489, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17560150, Length=274, Percent_Identity=30.6569343065693, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=30.5970149253731, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=30.7116104868914, Blast_Score=103, Evalue=6e-23, Organism=Caenorhabditis elegans, GI17562990, Length=274, Percent_Identity=31.7518248175182, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=30.6273062730627, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=32.7137546468402, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17563726, Length=269, Percent_Identity=30.8550185873606, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17560332, Length=276, Percent_Identity=32.6086956521739, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17565030, Length=275, Percent_Identity=33.0909090909091, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17538182, Length=267, Percent_Identity=31.8352059925094, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17560220, Length=276, Percent_Identity=30.7971014492754, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=28.9473684210526, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17559104, Length=273, Percent_Identity=31.1355311355311, Blast_Score=98, Evalue=4e-21, Organism=Caenorhabditis elegans, GI193203117, Length=247, Percent_Identity=29.5546558704453, Blast_Score=97, Evalue=6e-21, Organism=Caenorhabditis elegans, GI17564282, Length=202, Percent_Identity=30.1980198019802, Blast_Score=96, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=28.1954887218045, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI193203115, Length=248, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=33.0855018587361, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17536025, Length=256, Percent_Identity=29.6875, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=29.7297297297297, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=31.1023622047244, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17536651, Length=257, Percent_Identity=29.1828793774319, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17568967, Length=192, Percent_Identity=33.8541666666667, Blast_Score=84, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=27.4111675126904, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17567345, Length=198, Percent_Identity=31.3131313131313, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17561272, Length=260, Percent_Identity=29.6153846153846, Blast_Score=74, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17508651, Length=253, Percent_Identity=27.6679841897233, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI115534660, Length=197, Percent_Identity=27.9187817258883, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=189, Percent_Identity=31.7460317460317, Blast_Score=83, Evalue=5e-17, Organism=Saccharomyces cerevisiae, GI6323882, Length=195, Percent_Identity=34.8717948717949, Blast_Score=80, Evalue=4e-16, Organism=Saccharomyces cerevisiae, GI6324126, Length=260, Percent_Identity=24.6153846153846, Blast_Score=62, Evalue=6e-11, Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=36.5758754863813, Blast_Score=140, Evalue=9e-34, Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=34.765625, Blast_Score=117, Evalue=6e-27, Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=36.328125, Blast_Score=115, Evalue=3e-26, Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=33.0677290836653, Blast_Score=104, Evalue=7e-23, Organism=Drosophila melanogaster, GI17737361, Length=236, Percent_Identity=31.7796610169492, Blast_Score=100, Evalue=7e-22, Organism=Drosophila melanogaster, GI21355319, Length=258, Percent_Identity=30.2325581395349, Blast_Score=100, Evalue=8e-22, Organism=Drosophila melanogaster, GI24644339, Length=261, Percent_Identity=31.8007662835249, Blast_Score=97, Evalue=1e-20, Organism=Drosophila melanogaster, GI24643142, Length=255, Percent_Identity=30.9803921568627, Blast_Score=94, Evalue=8e-20, Organism=Drosophila melanogaster, GI281364892, Length=224, Percent_Identity=29.9107142857143, Blast_Score=87, Evalue=9e-18, Organism=Drosophila melanogaster, GI24644337, Length=258, Percent_Identity=32.1705426356589, Blast_Score=85, Evalue=4e-17, Organism=Drosophila melanogaster, GI18859837, Length=201, Percent_Identity=32.3383084577114, Blast_Score=80, Evalue=1e-15, Organism=Drosophila melanogaster, GI24641232, Length=199, Percent_Identity=30.1507537688442, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI24640492, Length=240, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=8e-15, Organism=Drosophila melanogaster, GI221510726, Length=223, Percent_Identity=29.1479820627803, Blast_Score=76, Evalue=3e-14, Organism=Drosophila melanogaster, GI21358495, Length=195, Percent_Identity=29.7435897435897, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI28571387, Length=210, Percent_Identity=29.0476190476191, Blast_Score=73, Evalue=2e-13, Organism=Drosophila melanogaster, GI21357673, Length=193, Percent_Identity=27.4611398963731, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI24580925, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24584748, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI161076933, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI21355631, Length=176, Percent_Identity=27.8409090909091, Blast_Score=69, Evalue=5e-12, Organism=Drosophila melanogaster, GI116007236, Length=204, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=7e-12, Organism=Drosophila melanogaster, GI116007234, Length=222, Percent_Identity=29.2792792792793, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI161076371, Length=203, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI24665243, Length=201, Percent_Identity=31.3432835820896, Blast_Score=66, Evalue=3e-11, Organism=Drosophila melanogaster, GI24640442, Length=201, Percent_Identity=30.8457711442786, Blast_Score=64, Evalue=7e-11, Organism=Drosophila melanogaster, GI24665264, Length=193, Percent_Identity=26.9430051813472, Blast_Score=64, Evalue=9e-11, Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=27.6041666666667, Blast_Score=64, Evalue=1e-10,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: =1.1.1.14 [H]
Molecular weight: Translated: 27187; Mature: 27056
Theoretical pI: Translated: 4.84; Mature: 4.84
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTDTETGRRLSGKVALVTGGASGIGKAVCQRFAAEGAKVVVADLDAGKCVAVAEAIGADA CCCCCCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCC WGVALDVTRQDSIEDAVRFSIAAAGKIDILVNAAGIYEVESILEISRERTAKVFQVNIEG CEEEEEECCCCCHHHHHHHHEEECCEEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCH LIFMTQVVARHMVERGEGGRIINFSSQAGRRGEGPAVAYCASKAAVISITQSCALELIRY HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHH GINVNAIAPGVVDTPMWDIVDAKLGSREGLQPGEVKARVAAAVPAGRFGAAEEQAAMAAF CCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHHH LAGPDAAYIVAQCYNVDGGNVMS HCCCCCEEEEEEEEECCCCCCCC >Mature Secondary Structure TDTETGRRLSGKVALVTGGASGIGKAVCQRFAAEGAKVVVADLDAGKCVAVAEAIGADA CCCCCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCC WGVALDVTRQDSIEDAVRFSIAAAGKIDILVNAAGIYEVESILEISRERTAKVFQVNIEG CEEEEEECCCCCHHHHHHHHEEECCEEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCH LIFMTQVVARHMVERGEGGRIINFSSQAGRRGEGPAVAYCASKAAVISITQSCALELIRY HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHH GINVNAIAPGVVDTPMWDIVDAKLGSREGLQPGEVKARVAAAVPAGRFGAAEEQAAMAAF CCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHHH LAGPDAAYIVAQCYNVDGGNVMS HCCCCCEEEEEEEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7551049 [H]