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The map label for this gene is rbsB [H]

Identifier: 209547736

GI number: 209547736

Start: 117408

End: 118427

Strand: Direct

Name: rbsB [H]

Synonym: Rleg2_0125

Alternate gene names: 209547736

Gene position: 117408-118427 (Clockwise)

Preceding gene: 209547733

Following gene: 209547737

Centisome position: 2.59

GC content: 62.35

Gene sequence:

>1020_bases
ATGAAGAAATCAGTTCTCGCTTTCGGCGCGCTCGCGCTTGGTGTCGCTTTTTCCGCGCCTGTCATGGCGGCTGACGTTTC
GGCCTGCCTGATCACCAAGACCGACACCAACCCCTTCTTCGTCAAGATGAAGGAAGGTGCGACGGCCAAGGCCAAGGAAC
TCGGCGTCTCGCTGAAGTCCTATGCCGGCAAGATCGACGGTGACAGCGAAAGCCAGGTGGCAGCGATCGAAAGCTGCATC
GCCGACGGCGCCAAGGGCATTCTGCTCACGGCTTCGGACACCAAGGGCATCGTGCCTGCGGTCAAGAAGGCCCGCGACGC
CGGCCTGCTGGTCATCGCTCTCGACACGCCGCTCGAGCCGGCCGATGCTGCCGACGCCACCTTCGCCACCGACAACCTGC
TCGCCGGCAAGCTGATCGGCCAGTGGGCCAAGGAAACCATGGGCGACAAGGCCAAGGACGCCAAGGTCGGCTTCCTCGAC
CTGACGCCGTCGCAGCCGACGGTCGACGTTCTCCGTGACCAGGGCTTCATGATGGGCTTCGGCATCGACCCGAAGAACCC
GGACAAGATCGGCGACGAAGACGACAAGCGCATCGTCGGTCACGACGTGACCAACGGCAATGAAGAAGGCGGCCGCAAGG
CCATGGAAAACCTTCTGCAGAAGGATCCGGGCATCAACGTCATCCACACGATCAACGAGCCGGCCGCTGTCGGCGCCTAT
CAGGCGCTGAAGGCCGTCGGCATGGAAAAGAACGTGCTGATCGTCTCCGTCGACGGCGGTTGCCCGGGTGTGAAGTCGGT
TAAGGAAGGCGTCATTGGCGCCACCTCGCAGCAATATCCGCTGCTGATGGCGTCGCTCGGTATCGAGGCGATCAAGAAGT
TCGCCGATAGCGGCGAAAAGCCGAAGCCGACCGAAGGCAAGTCCTTCTTCGACACCGGCGTCTCGCTCGTCACCGACAAG
CCGGTTTCCGGCGTCAAGTCGATCGACACCAAGGAAGGCACGGATAAGTGCTGGGGCTGA

Upstream 100 bases:

>100_bases
GCACGGGACGGCTTTGGAGGCTTAACAATCCACGGCGAAGTGCCATATCTTAAAATTCCGGCCCGCACGGGCGGCGCCGG
CAAACTCTGGGAGGAGTTCC

Downstream 100 bases:

>100_bases
GCCCAATCTCTTGAAACCAGAGCGGCCGGGGCTTGATCCCCGGCCGTTTTCGACGGACGATGTACCGTCGCCCCACGAAC
CGGCTCAGAATAGACCGGAA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKKSVLAFGALALGVAFSAPVMAADVSACLITKTDTNPFFVKMKEGATAKAKELGVSLKSYAGKIDGDSESQVAAIESCI
ADGAKGILLTASDTKGIVPAVKKARDAGLLVIALDTPLEPADAADATFATDNLLAGKLIGQWAKETMGDKAKDAKVGFLD
LTPSQPTVDVLRDQGFMMGFGIDPKNPDKIGDEDDKRIVGHDVTNGNEEGGRKAMENLLQKDPGINVIHTINEPAAVGAY
QALKAVGMEKNVLIVSVDGGCPGVKSVKEGVIGATSQQYPLLMASLGIEAIKKFADSGEKPKPTEGKSFFDTGVSLVTDK
PVSGVKSIDTKEGTDKCWG

Sequences:

>Translated_339_residues
MKKSVLAFGALALGVAFSAPVMAADVSACLITKTDTNPFFVKMKEGATAKAKELGVSLKSYAGKIDGDSESQVAAIESCI
ADGAKGILLTASDTKGIVPAVKKARDAGLLVIALDTPLEPADAADATFATDNLLAGKLIGQWAKETMGDKAKDAKVGFLD
LTPSQPTVDVLRDQGFMMGFGIDPKNPDKIGDEDDKRIVGHDVTNGNEEGGRKAMENLLQKDPGINVIHTINEPAAVGAY
QALKAVGMEKNVLIVSVDGGCPGVKSVKEGVIGATSQQYPLLMASLGIEAIKKFADSGEKPKPTEGKSFFDTGVSLVTDK
PVSGVKSIDTKEGTDKCWG
>Mature_339_residues
MKKSVLAFGALALGVAFSAPVMAADVSACLITKTDTNPFFVKMKEGATAKAKELGVSLKSYAGKIDGDSESQVAAIESCI
ADGAKGILLTASDTKGIVPAVKKARDAGLLVIALDTPLEPADAADATFATDNLLAGKLIGQWAKETMGDKAKDAKVGFLD
LTPSQPTVDVLRDQGFMMGFGIDPKNPDKIGDEDDKRIVGHDVTNGNEEGGRKAMENLLQKDPGINVIHTINEPAAVGAY
QALKAVGMEKNVLIVSVDGGCPGVKSVKEGVIGATSQQYPLLMASLGIEAIKKFADSGEKPKPTEGKSFFDTGVSLVTDK
PVSGVKSIDTKEGTDKCWG

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=300, Percent_Identity=30, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790526, Length=303, Percent_Identity=26.0726072607261, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790674, Length=278, Percent_Identity=25.8992805755396, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35243; Mature: 35243

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVLAFGALALGVAFSAPVMAADVSACLITKTDTNPFFVKMKEGATAKAKELGVSLKS
CCCCHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCHHH
YAGKIDGDSESQVAAIESCIADGAKGILLTASDTKGIVPAVKKARDAGLLVIALDTPLEP
HCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCC
ADAADATFATDNLLAGKLIGQWAKETMGDKAKDAKVGFLDLTPSQPTVDVLRDQGFMMGF
CCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCEEEEE
GIDPKNPDKIGDEDDKRIVGHDVTNGNEEGGRKAMENLLQKDPGINVIHTINEPAAVGAY
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
QALKAVGMEKNVLIVSVDGGCPGVKSVKEGVIGATSQQYPLLMASLGIEAIKKFADSGEK
HHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCC
PKPTEGKSFFDTGVSLVTDKPVSGVKSIDTKEGTDKCWG
CCCCCCCHHHHCCCEEEECCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKSVLAFGALALGVAFSAPVMAADVSACLITKTDTNPFFVKMKEGATAKAKELGVSLKS
CCCCHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCHHH
YAGKIDGDSESQVAAIESCIADGAKGILLTASDTKGIVPAVKKARDAGLLVIALDTPLEP
HCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCC
ADAADATFATDNLLAGKLIGQWAKETMGDKAKDAKVGFLDLTPSQPTVDVLRDQGFMMGF
CCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCEEEEE
GIDPKNPDKIGDEDDKRIVGHDVTNGNEEGGRKAMENLLQKDPGINVIHTINEPAAVGAY
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
QALKAVGMEKNVLIVSVDGGCPGVKSVKEGVIGATSQQYPLLMASLGIEAIKKFADSGEK
HHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCC
PKPTEGKSFFDTGVSLVTDKPVSGVKSIDTKEGTDKCWG
CCCCCCCHHHHCCCEEEECCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]