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The map label for this gene is dapE [H]

Identifier: 209547673

GI number: 209547673

Start: 61172

End: 62365

Strand: Reverse

Name: dapE [H]

Synonym: Rleg2_0061

Alternate gene names: 209547673

Gene position: 62365-61172 (Counterclockwise)

Preceding gene: 209547674

Following gene: 209547672

Centisome position: 1.37

GC content: 65.75

Gene sequence:

>1194_bases
ATGACCGCTACCGATCCCGTCGCCAATCTTCAGACGCTGATTCGCTGCCCCTCGGTGACTCCAGCCGAAGGCGGCGCGCT
GTCGGCGCTCGACGCCATGCTTGCGCCGCTCGGCTTCACCGTCGACAAGGTGAAAGCGAGCGAAGCGGGAACCGCCGATA
TTGAAAACCTCTATGCCCGTCTCGGCAAGGACGGCCCGCATCTGATGTTTGCCGGCCACACCGATGTCGTGCCGGTCGGC
GACGAGGCCGACTGGACGCATCCGCCCTTCGCCGCCGAGATATCGAAGGGCGAGCTCTTCGGCCGCGGTGCCGTCGACAT
GAAGGGCGGCATCGCCTGCTTCATCGCCGCCGTCGCCCGCCATATCGAGAAAAACGGGCCGCCCAAGGGCTCGATTTCCT
TCCTGATCACCGGCGATGAGGAAGGCCCGGCGATCAACGGCACAATCAAGCTTCTGCGGTGGGCGGCCGAACGCGGCGAG
CGCTGGGATGCCTGCCTGGTCGGCGAGCCCACCAATCCGGACAGGCTGGGCGACATGATCAAGATCGGCCGGCGCGGCTC
GCTGTCGGGCAAGATCACCGTGCACGGCGTCCAGGGCCATGCCGCCTATCCGCATCTTGCCGACAATCCGGTGCGCGGCA
TGCTGCAGCTGACGCAGGCGCTGATGGATCCGCCCTTCGACGGCGGCACCGACGATTTCCAGCCCTCGAACCTCGAGGTG
ACCACGGTCGATGTCGGCAATCCGGCGACCAACGTCATTCCGGCCAAGGCATCGGCCAGCTTCAACATCCGCTTCAACGA
CAGCTGGACGGCCGAGACGCTGCGGGCCGAAATCCTGCGGCGCCTCGACGCCGCCGCCGGAGACGGCCGGCTGCGGCCGG
GCCGCGAACCGGTGAAATACGATATCGTCTGGGCCGACCGGCCGAGCCATGTCTTCCTGACCCGCAACAATGCCCTGATC
GCGTCGCTGTCATCAGCCGTCGAAGGCGTGATCGGCCGCTCGCCTAAGCTTTCCACAACCGGCGGCACCTCGGATGCGCG
TTTCATCAAGGATTATTGCCCGGTCGTCGAGTTCGGCCTCGTCGGCCAGACGATGCACATGGTCGACGAGCGCGTCGCCG
TTTCCGATCTGGAGACGCTGACGGCGATCTACGAGACCTTCATTGCCCACTGGTTCGCCAATGCCGGGGCTTAG

Upstream 100 bases:

>100_bases
ATGACACCAGGACATTGATCCGGTAAACAGGCGGCGTCTTTTACGCGAGGCGCCGCCGCCTTGCCATGTCTTTGCCATTC
CCGCCGGATAGTTCGACGCC

Downstream 100 bases:

>100_bases
GGAAGTCCAATACTATCTGGCCGGCCTCTGGCTGCTGATCCGGCTGGATCCGCGCGGCTTCCGCTACCTCGATTTGTCCG
AGCGCGGCGTCAACCGCTCG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTATDPVANLQTLIRCPSVTPAEGGALSALDAMLAPLGFTVDKVKASEAGTADIENLYARLGKDGPHLMFAGHTDVVPVG
DEADWTHPPFAAEISKGELFGRGAVDMKGGIACFIAAVARHIEKNGPPKGSISFLITGDEEGPAINGTIKLLRWAAERGE
RWDACLVGEPTNPDRLGDMIKIGRRGSLSGKITVHGVQGHAAYPHLADNPVRGMLQLTQALMDPPFDGGTDDFQPSNLEV
TTVDVGNPATNVIPAKASASFNIRFNDSWTAETLRAEILRRLDAAAGDGRLRPGREPVKYDIVWADRPSHVFLTRNNALI
ASLSSAVEGVIGRSPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVSDLETLTAIYETFIAHWFANAGA

Sequences:

>Translated_397_residues
MTATDPVANLQTLIRCPSVTPAEGGALSALDAMLAPLGFTVDKVKASEAGTADIENLYARLGKDGPHLMFAGHTDVVPVG
DEADWTHPPFAAEISKGELFGRGAVDMKGGIACFIAAVARHIEKNGPPKGSISFLITGDEEGPAINGTIKLLRWAAERGE
RWDACLVGEPTNPDRLGDMIKIGRRGSLSGKITVHGVQGHAAYPHLADNPVRGMLQLTQALMDPPFDGGTDDFQPSNLEV
TTVDVGNPATNVIPAKASASFNIRFNDSWTAETLRAEILRRLDAAAGDGRLRPGREPVKYDIVWADRPSHVFLTRNNALI
ASLSSAVEGVIGRSPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVSDLETLTAIYETFIAHWFANAGA
>Mature_396_residues
TATDPVANLQTLIRCPSVTPAEGGALSALDAMLAPLGFTVDKVKASEAGTADIENLYARLGKDGPHLMFAGHTDVVPVGD
EADWTHPPFAAEISKGELFGRGAVDMKGGIACFIAAVARHIEKNGPPKGSISFLITGDEEGPAINGTIKLLRWAAERGER
WDACLVGEPTNPDRLGDMIKIGRRGSLSGKITVHGVQGHAAYPHLADNPVRGMLQLTQALMDPPFDGGTDDFQPSNLEVT
TVDVGNPATNVIPAKASASFNIRFNDSWTAETLRAEILRRLDAAAGDGRLRPGREPVKYDIVWADRPSHVFLTRNNALIA
SLSSAVEGVIGRSPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVSDLETLTAIYETFIAHWFANAGA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=392, Percent_Identity=40.0510204081633, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1790395, Length=406, Percent_Identity=25.8620689655172, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789236, Length=400, Percent_Identity=22.25, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42290; Mature: 42159

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00092 N6_MTASE ; PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATDPVANLQTLIRCPSVTPAEGGALSALDAMLAPLGFTVDKVKASEAGTADIENLYAR
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHH
LGKDGPHLMFAGHTDVVPVGDEADWTHPPFAAEISKGELFGRGAVDMKGGIACFIAAVAR
HCCCCCEEEEECCCCEEECCCCCCCCCCCCCEECCCCCEECCCCCCCCCCHHHHHHHHHH
HIEKNGPPKGSISFLITGDEEGPAINGTIKLLRWAAERGERWDACLVGEPTNPDRLGDMI
HHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KIGRRGSLSGKITVHGVQGHAAYPHLADNPVRGMLQLTQALMDPPFDGGTDDFQPSNLEV
HHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TTVDVGNPATNVIPAKASASFNIRFNDSWTAETLRAEILRRLDAAAGDGRLRPGREPVKY
EEEECCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
DIVWADRPSHVFLTRNNALIASLSSAVEGVIGRSPKLSTTGGTSDARFIKDYCPVVEFGL
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
VGQTMHMVDERVAVSDLETLTAIYETFIAHWFANAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TATDPVANLQTLIRCPSVTPAEGGALSALDAMLAPLGFTVDKVKASEAGTADIENLYAR
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHH
LGKDGPHLMFAGHTDVVPVGDEADWTHPPFAAEISKGELFGRGAVDMKGGIACFIAAVAR
HCCCCCEEEEECCCCEEECCCCCCCCCCCCCEECCCCCEECCCCCCCCCCHHHHHHHHHH
HIEKNGPPKGSISFLITGDEEGPAINGTIKLLRWAAERGERWDACLVGEPTNPDRLGDMI
HHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KIGRRGSLSGKITVHGVQGHAAYPHLADNPVRGMLQLTQALMDPPFDGGTDDFQPSNLEV
HHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TTVDVGNPATNVIPAKASASFNIRFNDSWTAETLRAEILRRLDAAAGDGRLRPGREPVKY
EEEECCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
DIVWADRPSHVFLTRNNALIASLSSAVEGVIGRSPKLSTTGGTSDARFIKDYCPVVEFGL
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
VGQTMHMVDERVAVSDLETLTAIYETFIAHWFANAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA