Click here to switch to the map view.

The map label for this gene is truA

Identifier: 209547671

GI number: 209547671

Start: 59776

End: 60582

Strand: Direct

Name: truA

Synonym: Rleg2_0059

Alternate gene names: 209547671

Gene position: 59776-60582 (Clockwise)

Preceding gene: 209547670

Following gene: 209547675

Centisome position: 1.32

GC content: 66.79

Gene sequence:

>807_bases
ATGCCGCGTTTCCGCATGACGGTCGAATATGACGGCGGCCCCTATGTCGGCTGGCAGAGGCAGGAGAATGGCCCCTCGGT
GCAGGGCGCGATCGAAGCCGCCGTGCTGTCGCTGACCGGCGAGACTGTCTCGATCCGCGGCGCCGGCCGCACCGATTCCG
GCGTCCACGCCATGGGGCAGGTGATCCATGCCGATCTTTCGAAGGCGTGGTCGCCCTACCAGCTGCAGAATGCGCTGAAC
GCGCATTTGAGGCTTGCCGGCGAGCGCGTCTCCATTCTCGATGTCGAGGCGGTTGCGGAATTCTTCGATGCCCGTTTTTC
GGCGCTGCGCCGCCACTATCTCTACCGCATCGTCAGCCGCCGGGCGCCGCTGGCGCTTGAAGCCGGCAAGGCCTGGTGGG
TGCCGAAGGTGCTCGATCACGAGGTCATGCACGCCGCCGCCCAGAGGCTGGTCGGCCGGCACGATTTCTCCACCTTCCGC
GCCGCCCACTGCCAGGCAAACAGCCCGGTGCGCACGCTCGACCGGCTGGATGTGACACGCAGCGGCGAGCTGATCGAGAT
CCGCGCCACGGCGCAGAGTTTTCTCCACAACCAGATCCGCTCCTTCGCCGGCACGCTGAAGCTCGCCGGCGAAGGCAAAT
GGACGCCTGACGATGTCGAGGCCGCCCTTGAGGCGCGCGACCGCAAGGCCTGCGGCCCGGTGGCGCCGCCAGACGGGCTC
TATTTCCTGCAGGTGGATTATCCCGACGTGATCCCCGACCGTCGCAGGCCGGTCACTGATGCCGATGACGGCGACAATCT
GTCGTAA

Upstream 100 bases:

>100_bases
CTGTGCGGCTGACCAGGCTGCAGAAGGCAGGCGGCAAGCCGCTGGCGGCGGCCGATTTCCTCAGGGGCACGCCGCTTGCC
GCCGGCATGAGGCTTTCCTG

Downstream 100 bases:

>100_bases
ACCCGCTCAGACCGGATAGATGCCGAGGAAGTGCTGCAGATATTCCGAAAGGATCATCGACGGCACCAGCAGCACCAGCG
TCATTGCCCCGACCATCAGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPRFRMTVEYDGGPYVGWQRQENGPSVQGAIEAAVLSLTGETVSIRGAGRTDSGVHAMGQVIHADLSKAWSPYQLQNALN
AHLRLAGERVSILDVEAVAEFFDARFSALRRHYLYRIVSRRAPLALEAGKAWWVPKVLDHEVMHAAAQRLVGRHDFSTFR
AAHCQANSPVRTLDRLDVTRSGELIEIRATAQSFLHNQIRSFAGTLKLAGEGKWTPDDVEAALEARDRKACGPVAPPDGL
YFLQVDYPDVIPDRRRPVTDADDGDNLS

Sequences:

>Translated_268_residues
MPRFRMTVEYDGGPYVGWQRQENGPSVQGAIEAAVLSLTGETVSIRGAGRTDSGVHAMGQVIHADLSKAWSPYQLQNALN
AHLRLAGERVSILDVEAVAEFFDARFSALRRHYLYRIVSRRAPLALEAGKAWWVPKVLDHEVMHAAAQRLVGRHDFSTFR
AAHCQANSPVRTLDRLDVTRSGELIEIRATAQSFLHNQIRSFAGTLKLAGEGKWTPDDVEAALEARDRKACGPVAPPDGL
YFLQVDYPDVIPDRRRPVTDADDGDNLS
>Mature_267_residues
PRFRMTVEYDGGPYVGWQRQENGPSVQGAIEAAVLSLTGETVSIRGAGRTDSGVHAMGQVIHADLSKAWSPYQLQNALNA
HLRLAGERVSILDVEAVAEFFDARFSALRRHYLYRIVSRRAPLALEAGKAWWVPKVLDHEVMHAAAQRLVGRHDFSTFRA
AHCQANSPVRTLDRLDVTRSGELIEIRATAQSFLHNQIRSFAGTLKLAGEGKWTPDDVEAALEARDRKACGPVAPPDGLY
FLQVDYPDVIPDRRRPVTDADDGDNLS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=250, Percent_Identity=35.2, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=36.3636363636364, Blast_Score=156, Evalue=1e-39,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=27.2131147540984, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_RHILW (B5ZN21)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002279588.1
- ProteinModelPortal:   B5ZN21
- SMR:   B5ZN21
- GeneID:   6978768
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_0059
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29666; Mature: 29535

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFRMTVEYDGGPYVGWQRQENGPSVQGAIEAAVLSLTGETVSIRGAGRTDSGVHAMGQ
CCCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHH
VIHADLSKAWSPYQLQNALNAHLRLAGERVSILDVEAVAEFFDARFSALRRHYLYRIVSR
HHHHHHHHCCCCHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
RAPLALEAGKAWWVPKVLDHEVMHAAAQRLVGRHDFSTFRAAHCQANSPVRTLDRLDVTR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCC
SGELIEIRATAQSFLHNQIRSFAGTLKLAGEGKWTPDDVEAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
YFLQVDYPDVIPDRRRPVTDADDGDNLS
EEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRFRMTVEYDGGPYVGWQRQENGPSVQGAIEAAVLSLTGETVSIRGAGRTDSGVHAMGQ
CCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHH
VIHADLSKAWSPYQLQNALNAHLRLAGERVSILDVEAVAEFFDARFSALRRHYLYRIVSR
HHHHHHHHCCCCHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
RAPLALEAGKAWWVPKVLDHEVMHAAAQRLVGRHDFSTFRAAHCQANSPVRTLDRLDVTR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCC
SGELIEIRATAQSFLHNQIRSFAGTLKLAGEGKWTPDDVEAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
YFLQVDYPDVIPDRRRPVTDADDGDNLS
EEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA