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The map label for this gene is sppA [H]

Identifier: 209547656

GI number: 209547656

Start: 44932

End: 45882

Strand: Reverse

Name: sppA [H]

Synonym: Rleg2_0044

Alternate gene names: 209547656

Gene position: 45882-44932 (Counterclockwise)

Preceding gene: 209547664

Following gene: 209547655

Centisome position: 1.01

GC content: 61.41

Gene sequence:

>951_bases
ATGGACAGTTCGATCATCGCCGATCGCCGGCGGCTGCGCCGCAAGCTCGGCTTCTGGCGTATCGTCGCGGTCGCCCTCAT
CGTGGCGCTTGGCCTGGCCTTTTACAGCTTCGCCTTCGGCAATGCCGGAACGGAGCGCCCGCATATCGCCCATGTGACGA
TATCGGGGCTGATCGTCGACGATGACGAGCTTCTAGAGCGGCTGAAGAAGGTCGAGACCAGCGATCAGGTGAAGGCCGCG
GTGATTTCGATCTCCTCGCCCGGCGGCACCACCTATGGCGGCGAAAAGATCTTCAAGGCGATCCGCGCGATCGCAGCCAA
GAAACCCGTCGTCTCCGACGTGCGCACGCTTGCCGCCTCCGCCGGCTACATGATCGCCACCGCCGGCGATACGATCATTG
CCGGCGACAGCTCGATCACCGGCTCGATCGGCGTCATCTTCCAGTATCCGCAGATCCAGCCGCTGCTCGACAAGATCGGC
GTCTCGCTGCAGGAGATCAAATCCTCGCCGCTGAAGGCCGAGCCCTCGCCGTTCCATGAAGCGAGCGAGGAGGCCAAGGC
AATGATCCGCAACATGGTGGTCGACAGCTATAACTGGTTCGTCGATCTGGTCGCCGACCGGCGCAAGCTGCCGCGCGACG
AGGTGCTGAAACTCGCCGACGGCACGATCTATACCGGCCGCCAGGCGCTCAAGGTGAAACTCGTCGATACGATCGGCGGC
GAGCCGGAAATCCGGGCCTATCTCAAATCGCGCGGCGTCGACGCCGACCTGCCGATGGTCGACTGGGACAAGAAGAGCAA
TACGCCCTTTCTGCTCGCTGGGGCTGTTTCGCGGTTGATTACGATCTTCGGTTATGATGATCTCTTGAAAGGCCAGGATA
TCAATGGAATCCTGCCCCCAAAGTTGCTTCTTGACGGGCTGCTTTCAGTTTGGCAGGTTGGCCGCGATTGA

Upstream 100 bases:

>100_bases
GGAAAAACATCAAAAAGTGGCATTCGTACGCGCCGCTGCCACCTATTTCACCGGATCGATCGGCGGCATGCCGATGCCGG
GCCAGCGCTGGAGGTTTCTC

Downstream 100 bases:

>100_bases
TAAGAAATCAATAATTTAAGGGGGCGAACGTGATCAAGTCCGAATTGGTGCAGATTGTTGCAGCGCGCAACCCGCATCTC
TATCATCGCGACGTCGAGAA

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MDSSIIADRRRLRRKLGFWRIVAVALIVALGLAFYSFAFGNAGTERPHIAHVTISGLIVDDDELLERLKKVETSDQVKAA
VISISSPGGTTYGGEKIFKAIRAIAAKKPVVSDVRTLAASAGYMIATAGDTIIAGDSSITGSIGVIFQYPQIQPLLDKIG
VSLQEIKSSPLKAEPSPFHEASEEAKAMIRNMVVDSYNWFVDLVADRRKLPRDEVLKLADGTIYTGRQALKVKLVDTIGG
EPEIRAYLKSRGVDADLPMVDWDKKSNTPFLLAGAVSRLITIFGYDDLLKGQDINGILPPKLLLDGLLSVWQVGRD

Sequences:

>Translated_316_residues
MDSSIIADRRRLRRKLGFWRIVAVALIVALGLAFYSFAFGNAGTERPHIAHVTISGLIVDDDELLERLKKVETSDQVKAA
VISISSPGGTTYGGEKIFKAIRAIAAKKPVVSDVRTLAASAGYMIATAGDTIIAGDSSITGSIGVIFQYPQIQPLLDKIG
VSLQEIKSSPLKAEPSPFHEASEEAKAMIRNMVVDSYNWFVDLVADRRKLPRDEVLKLADGTIYTGRQALKVKLVDTIGG
EPEIRAYLKSRGVDADLPMVDWDKKSNTPFLLAGAVSRLITIFGYDDLLKGQDINGILPPKLLLDGLLSVWQVGRD
>Mature_316_residues
MDSSIIADRRRLRRKLGFWRIVAVALIVALGLAFYSFAFGNAGTERPHIAHVTISGLIVDDDELLERLKKVETSDQVKAA
VISISSPGGTTYGGEKIFKAIRAIAAKKPVVSDVRTLAASAGYMIATAGDTIIAGDSSITGSIGVIFQYPQIQPLLDKIG
VSLQEIKSSPLKAEPSPFHEASEEAKAMIRNMVVDSYNWFVDLVADRRKLPRDEVLKLADGTIYTGRQALKVKLVDTIGG
EPEIRAYLKSRGVDADLPMVDWDKKSNTPFLLAGAVSRLITIFGYDDLLKGQDINGILPPKLLLDGLLSVWQVGRD

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=218, Percent_Identity=32.5688073394495, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787527, Length=184, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 34377; Mature: 34377

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSIIADRRRLRRKLGFWRIVAVALIVALGLAFYSFAFGNAGTERPHIAHVTISGLIVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEC
DDELLERLKKVETSDQVKAAVISISSPGGTTYGGEKIFKAIRAIAAKKPVVSDVRTLAAS
CHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
AGYMIATAGDTIIAGDSSITGSIGVIFQYPQIQPLLDKIGVSLQEIKSSPLKAEPSPFHE
CCEEEEECCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCCCHH
ASEEAKAMIRNMVVDSYNWFVDLVADRRKLPRDEVLKLADGTIYTGRQALKVKLVDTIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCCCEEEEEEEHHCCC
EPEIRAYLKSRGVDADLPMVDWDKKSNTPFLLAGAVSRLITIFGYDDLLKGQDINGILPP
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHCCCCCCCCCCH
KLLLDGLLSVWQVGRD
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSSIIADRRRLRRKLGFWRIVAVALIVALGLAFYSFAFGNAGTERPHIAHVTISGLIVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEC
DDELLERLKKVETSDQVKAAVISISSPGGTTYGGEKIFKAIRAIAAKKPVVSDVRTLAAS
CHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
AGYMIATAGDTIIAGDSSITGSIGVIFQYPQIQPLLDKIGVSLQEIKSSPLKAEPSPFHE
CCEEEEECCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCCCHH
ASEEAKAMIRNMVVDSYNWFVDLVADRRKLPRDEVLKLADGTIYTGRQALKVKLVDTIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCCCEEEEEEEHHCCC
EPEIRAYLKSRGVDADLPMVDWDKKSNTPFLLAGAVSRLITIFGYDDLLKGQDINGILPP
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHCCCCCCCCCCH
KLLLDGLLSVWQVGRD
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]