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The map label for this gene is rnfG

Identifier: 209400825

GI number: 209400825

Start: 2216226

End: 2216846

Strand: Direct

Name: rnfG

Synonym: ECH74115_2343

Alternate gene names: 209400825

Gene position: 2216226-2216846 (Clockwise)

Preceding gene: 209398754

Following gene: 209396599

Centisome position: 39.77

GC content: 52.82

Gene sequence:

>621_bases
ATGCTGAAAACTATCCGAAAACACGGCATTACGTTGGCGCTATTTGCAGCGGGTTCAACAGGGTTAACTGCGGCCATCAA
CCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGCTTTATTTGATCAGGTGCTGCCAGCCGAAC
GCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATC
GCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCT
GGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAA
TCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCGGATGATGCGCACTGGGCGGTG
AAGAAAGATGGTGGTAATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGC
CGGATTGTACGCTCAGACGTTACCGGAACAACTTTCTCAACTTCCTGCCTGTGGAGAATAA

Upstream 100 bases:

>100_bases
TGACGGCGTGGCTTTTGCCGTCCTGCTGGCGAACATCACGGTTCCTCTGATCGATTACTACACGCGTCCGCGCGTCTACG
GCCATCGCAAAGGGTAAACC

Downstream 100 bases:

>100_bases
AGCGTGAGCGAAATTAAAGACGTTATTGTTCAGGGGTTGTGGAAAAACAACTCTGCGCTGGTCCAGTTGCTCGGCCTTTG
TCCTCTGTTGGCTGTCACGT

Product: electron transport complex protein RnfG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKALFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYI
AKQDDKPVAAVLEATAPDGYSGAIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE

Sequences:

>Translated_206_residues
MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKALFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYI
AKQDDKPVAAVLEATAPDGYSGAIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE
>Mature_206_residues
MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKALFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYI
AKQDDKPVAAVLEATAPDGYSGAIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4659

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfG

Gene ontology:

Cell location: Cell inner membrane (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rnfG family

Homologues:

Organism=Escherichia coli, GI1787918, Length=206, Percent_Identity=99.0291262135922, Blast_Score=419, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFG_ECO57 (P58345)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90921
- PIR:   H85769
- RefSeq:   NP_288067.1
- RefSeq:   NP_310367.1
- EnsemblBacteria:   EBESCT00000027637
- EnsemblBacteria:   EBESCT00000057206
- GeneID:   914445
- GeneID:   961583
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2640
- KEGG:   ecs:ECs2340
- GeneTree:   EBGT00050000010215
- HOGENOM:   HBG454413
- OMA:   GMFDQFT
- ProtClustDB:   PRK01908
- BioCyc:   ECOL83334:ECS2340-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00479
- InterPro:   IPR022989
- InterPro:   IPR010209
- InterPro:   IPR007329
- SMART:   SM00900
- TIGRFAMs:   TIGR01947

Pfam domain/function: PF04205 FMN_bind

EC number: NA

Molecular weight: Translated: 21969; Mature: 21969

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20ae0f44)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKALFDQVLPAERYNNALA
CCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSGAIQLLVGADFNGTVLGTR
HCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEE
VTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAVKKDGGNFDQFTGATITPRAV
ECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHH
VNAVKRAGLYAQTLPEQLSQLPACGE
HHHHHHCCCHHHHHHHHHHHCCCCCH
>Mature Secondary Structure
MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKALFDQVLPAERYNNALA
CCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSGAIQLLVGADFNGTVLGTR
HCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEE
VTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAVKKDGGNFDQFTGATITPRAV
ECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHH
VNAVKRAGLYAQTLPEQLSQLPACGE
HHHHHHCCCHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796