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The map label for this gene is rnfE

Identifier: 209396599

GI number: 209396599

Start: 2216850

End: 2217545

Strand: Direct

Name: rnfE

Synonym: ECH74115_2344

Alternate gene names: 209396599

Gene position: 2216850-2217545 (Clockwise)

Preceding gene: 209400825

Following gene: 209396011

Centisome position: 39.78

GC content: 52.3

Gene sequence:

>696_bases
GTGAGCGAAATTAAAGACGTTATTGTTCAGGGGTTGTGGAAAAACAACTCTGCGCTGGTCCAGTTGCTCGGCCTTTGTCC
TCTGTTGGCTGTCACGTCTACTGCCACTAACGCTCTGGGTTTAGGACTTGCGACTACGCTGGTACTGACGCTGACCAACC
TGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTC
AGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCAC
TAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCTGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAA
TTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGT
GCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGAT
GCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCC
GTACTGAAGCAGCTGCCGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGA

Upstream 100 bases:

>100_bases
TACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGTACGCTCAGACGTTACCGGAACAACTTTCTCAACTTC
CTGCCTGTGGAGAATAAAGC

Downstream 100 bases:

>100_bases
TGAACAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACTGAGCTTAATTTCAGT
TCGCCTTTTGAATTGCTGAT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLATTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAAAERALPNGETGNV

Sequences:

>Translated_231_residues
MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLATTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAAAERALPNGETGNV
>Mature_230_residues
SEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLATTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVS
AVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDGA
DALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAAAERALPNGETGNV

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=231, Percent_Identity=99.5670995670996, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=35.8974358974359, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_ECO24 (A7ZM92)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462921.1
- STRING:   A7ZM92
- EnsemblBacteria:   EBESCT00000019367
- GeneID:   5590687
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1841
- eggNOG:   COG4660
- GeneTree:   EBGT00050000011040
- HOGENOM:   HBG624915
- OMA:   ENPVTKM
- ProtClustDB:   PRK12405
- BioCyc:   ECOL331111:ECE24377A_1841-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 24489; Mature: 24358

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20472310)-; HASH(0x2076b238)-; HASH(0x20a6e93c)-; HASH(0x20af8584)-; HASH(0x2119fc2c)-; HASH(0x2128108c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLATTLVLTLTNLTISTLR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDGADALLGSWAKVLRVEIFHTD
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEHEEECC
SPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAAAERALPNGETGNV
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
SEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLATTLVLTLTNLTISTLR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDGADALLGSWAKVLRVEIFHTD
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEHEEECC
SPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAAAERALPNGETGNV
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA