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The map label for this gene is selU [H]

Identifier: 209400577

GI number: 209400577

Start: 625992

End: 627086

Strand: Reverse

Name: selU [H]

Synonym: ECH74115_0604

Alternate gene names: 209400577

Gene position: 627086-625992 (Counterclockwise)

Preceding gene: 209396184

Following gene: 209396189

Centisome position: 11.25

GC content: 55.07

Gene sequence:

>1095_bases
ATGCAAGAGAGACACACGGAACAGGACTATCGTGCCCTGCTGATTGCTGATACGCCCATTATTGATGTTCGCGCCCCTAT
CGAGTTTGAGCAAGGCGCAATGCCCGCCGCTATCAATCTGCCGTTAATGAATAACGATGAACGCGCCGCCGTTGGCACCT
GCTATAAACAGCAAGGCTCAGACGCAGCGCTGGCGCTGGGACATAAACTGGTTGCGGGTGAAATTCGTCAGCAGCGCATG
GACGCCTGGCGGGCAGCCTGCCTGCAAAATCCGCAAGGTATTCTCTGCTGCGCCCGTGGCGGTCAGCGCTCACATATTGT
GCAAAGCTGGTTGCATGCAGCGGGGATTGATTATCCGCTGGTGGAAGGCGGTTATAAGGCACTGCGCCAGACCGCGATTC
AGGCGACTATTGAACTGGCACAAAAACCGATAGTGCTGATTGGCGGTTGTACCGGCAGCGGTAAAACGCTGTTAGTGCAG
CAACAGCCGAACGGTGTTGATCTGGAAGGGTTGGCGCGTCATCGCGGTTCGGCGTTTGGTCGCACGTTACAACCACAACT
TAGCCAGGCGAGTTTTGAAAACCTGCTGGCTGCCGAAATGCTAAAAACCGACGCCCGTCAGAATTTGCGCCTGTGGGTGC
TGGAAGACGAAAGCCGGATGATCGGTTCGAATCACCTGCCGGAATGCCTGCGCGAGCGAATGACTCAGGCGGCGATTGCG
GTGGTAGAAGATCCGTTTGAGATCCGTCTTGAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCA
CGCGTACGGCGACGAACAGGGCTGGCAGGAATATTGCGAATACCTGCATCACGGACTTTCGGCTATTAAGCGTCGGCTGG
GGCTACAGCGATATAACGAACTGGCTGCAAGGCTGGATGCAGCACTGACAACGCAACTCACCACCGGCAGCACCGACGGT
CATCTGGCCTGGCTGGTGCCGTTACTTGAAGAATATTACGACCCGATGTATCGCTATCAGCTTGAGAAAAAAGCGGAAAA
AGTTGTCTTTCGCGGTGAGTGGGCGGAAGTGGCGGAATGGGTTAAGGCGCAGTAA

Upstream 100 bases:

>100_bases
TTTTAGAAATTTTCGGCAACCCACGCAGTTAAAAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGA
TTTGAGGTTCACTTTTAACT

Downstream 100 bases:

>100_bases
TGAGGGACGTATTTGTATTAGTCGCGACTTGATCTGACACATGGCCTTGAAAGGTTGAGAGTTACCGGTTTTGATATGAG
TGTCGAATCCTTATCCAAAA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ

Sequences:

>Translated_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ
>Mature_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=364, Percent_Identity=99.4505494505495, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41074; Mature: 41074

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHCCC
DAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKAQ
HCCC
>Mature Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHCCC
DAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKAQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA