Click here to switch to the map view.

The map label for this gene is allS

Identifier: 209396184

GI number: 209396184

Start: 627155

End: 628081

Strand: Reverse

Name: allS

Synonym: ECH74115_0605

Alternate gene names: 209396184

Gene position: 628081-627155 (Counterclockwise)

Preceding gene: 209395822

Following gene: 209400577

Centisome position: 11.27

GC content: 52.0

Gene sequence:

>927_bases
ATGTTCGATCCAGAAACCTTGCGGACTTTCATTGCGGTTGCTGAAACAGGAAGTTTTTCAAAAGCGGCAGAACGATTATG
TAAAACCACGGCGACGATCAGTTATCGCATTAAACTTCTGGAAGAGAATACCGGAGTGGCGCTGTTTTTTCGCACGACTC
GTAGCGTAACGTTGACAGCGGCTGGCGAGCATCTACTTTCCCAGGCCAGAGACTGGCTGAGCTGGCTGGAAAGTATGCCC
AGCGAGCTGCAACAGGTGAATGATGGCGTGGAACGCCAGGTGAATATTGTCATCAACAACCTGCTCTACAACCCCCAGGC
CGTCGCCCAGTTGCTGGCGTGGCTGAATGAACGTTACCCCTTTACCCAGTTTCACATCTCCCGACAAATCTATATGGGCG
TCTGGGACTCGCTATTGTACGAAGGTTTTTCACTGGCTATCGGCGTCACGGGAACCGAAGCGCTGGCAAATACCTTTAGT
CTTGATCCCTTAGGATCGGTGCAATGGCGATTTGTCATGGCGGCGGATCATCCGCTGGCGAACGTTGAAGAGCCGCTAAC
AGAAGCGCAGTTGCGGCGCTTTCCGGCGGTCAATATTGAAGACAGCGCCCGCACCTTAACCAAACGCGTCGCCTGGCGAT
TGCCAGGGCAAAAAGAGATTATTGTTCCTGATATGGAAACGAAAATCGCCGCCCATCTGGCGGGCGTTGGCATTGGTTTT
TTGCCAAAATCGCTTTGCCAGTCAATGATCGATAATCAACAACTGGTCAGCCGGGTAATCCCAACGATGCGCCCTCCTTC
GCCATTGAGTCTGGCATGGCGCAAATTTGGCAGCGGCAAAGCGGTAGAAGATATTGTGACCTTGTTTACCCAGCGCAGGC
CGGAAATCAGCGGATTTTTAGAAATTTTCGGCAACCCACGCAGTTAA

Upstream 100 bases:

>100_bases
TTTAGTCCACAAATTGAACAGGGTTATCATGTAAAAATACTGATAAATCAGAGGATAAGAAAATCACGCAACTTGCGGAT
TTAAAAGGGGATACCTGAAG

Downstream 100 bases:

>100_bases
AAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGATTTGAGGTTCACTTTTAACTATGCAAGAGAGA
CACACGGAACAGGACTATCG

Product: DNA-binding transcriptional activator AllS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS

Sequences:

>Translated_308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS
>Mature_308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS

Specific function: Positive regulator essential for the expression of AllD operon. Binds to the AllD promoter

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786713, Length=308, Percent_Identity=100, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1786508, Length=283, Percent_Identity=38.5159010600707, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1787949, Length=275, Percent_Identity=30.9090909090909, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87082024, Length=277, Percent_Identity=24.9097472924188, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789492, Length=281, Percent_Identity=26.6903914590747, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=30.1204819277108, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=258, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=32.5, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=29.1428571428571, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): ALLS_ECO57 (P0ACR1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E90699
- PIR:   H85549
- RefSeq:   NP_286252.1
- RefSeq:   NP_308592.1
- ProteinModelPortal:   P0ACR1
- SMR:   P0ACR1
- EnsemblBacteria:   EBESCT00000024823
- EnsemblBacteria:   EBESCT00000059565
- GeneID:   915486
- GeneID:   957494
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0658
- KEGG:   ecs:ECs0565
- GeneTree:   EBGT00050000008332
- HOGENOM:   HBG685425
- OMA:   VYMGVWD
- ProtClustDB:   PRK10094
- BioCyc:   ECOL83334:ECS0565-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34512; Mature: 34512

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC
>Mature Secondary Structure
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796