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The map label for this gene is gcl

Identifier: 209400464

GI number: 209400464

Start: 629776

End: 631557

Strand: Direct

Name: gcl

Synonym: ECH74115_0608

Alternate gene names: 209400464

Gene position: 629776-631557 (Clockwise)

Preceding gene: 209400082

Following gene: 209397047

Centisome position: 11.3

GC content: 53.59

Gene sequence:

>1782_bases
ATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACCACCGCCTTCGGCGTTCCGGG
AGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTG
CTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGC
ACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGC
CCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTC
GTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGTCCGGGTCCGGTACTGGTG
GATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGC
TGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGG
TAATCAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTGATGGGC
TGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTCGGTCTGCAAACCGCGCATCGTTACGGTAACGCAAC
GTTGCTGGCGTCCGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGTTCGGTAGAGAAATACACCG
AAGGGCGCAAAATCGTTCATATCGATATTGAGCCGACGCAAATTGGCCGCGTGCTGTGTCCGGATCTCGGCATTGTCTCT
GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGA
ATGGGTCGCCGATTGCCAGCAGCGCAAACGCACTTTGCTGCGCAAAACCCATTTCGACAACGTGCCGGTGAAACCGCAGC
GCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGCCTGTCACAAATCGCTGCG
GCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGC
GGCGCTGGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTG
AAGAGTTAGCCGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTGAATAACGCCTATCTCGGCCTGATTCGT
CAGTCACAACGCGCCTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGCGAAGTGAACGGTTA
CGGCGTCGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGCGTCTTCAAACCGGAAGATATTGCACCGG
CCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACAAAT
ATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGATGCACCGACTGAAAC
CTGCTTCATGCACTATGAATAA

Upstream 100 bases:

>100_bases
CACATCCATAGTGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAATTTTTCCAA
TTAATAGAGGTAGGAATAAA

Downstream 100 bases:

>100_bases
GGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGTGAATATGATTTTCTCGCCCGTTTTGAGAA
AGCTGCGCAGTGTGGTTTTC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVMEFEDIADNAADAPTETCFMHYE

Sequences:

>Translated_593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVMEFEDIADNAADAPTETCFMHYE
>Mature_592_residues
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGT
DMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVD
LPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGW
GCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSD
AKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQ
SQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNI
SMGSELDNVMEFEDIADNAADAPTETCFMHYE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde)

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=22.9824561403509, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI21361361, Length=601, Percent_Identity=24.459234608985, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=100, Blast_Score=1226, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=33.9788732394366, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=35.4771784232365, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=25.1824817518248, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788716, Length=474, Percent_Identity=25.9493670886076, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=494, Percent_Identity=24.6963562753036, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=494, Percent_Identity=24.6963562753036, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=25.2302025782689, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=31.3131313131313, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=23.4458259325044, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=505, Percent_Identity=23.1683168316832, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=21.7303822937626, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=24.2299794661191, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCL_ECO57 (P0AEP8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85550
- PIR:   H90699
- RefSeq:   NP_286255.1
- RefSeq:   NP_308595.1
- ProteinModelPortal:   P0AEP8
- SMR:   P0AEP8
- PRIDE:   P0AEP8
- EnsemblBacteria:   EBESCT00000028803
- EnsemblBacteria:   EBESCT00000060067
- GeneID:   915770
- GeneID:   957497
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0661
- KEGG:   ecs:ECs0568
- GeneTree:   EBGT00050000008285
- HOGENOM:   HBG323037
- OMA:   EGYTRAK
- ProtClustDB:   PRK11269
- BioCyc:   ECOL83334:ECS0568-MONOMER
- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR01504

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =4.1.1.47

Molecular weight: Translated: 64733; Mature: 64601

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796