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The map label for this gene is rluF

Identifier: 209400390

GI number: 209400390

Start: 5148100

End: 5148972

Strand: Direct

Name: rluF

Synonym: ECH74115_5500

Alternate gene names: 209400390

Gene position: 5148100-5148972 (Clockwise)

Preceding gene: 209399290

Following gene: 209399227

Centisome position: 92.39

GC content: 52.12

Gene sequence:

>873_bases
ATGCTGCCCGACTCATCAGTCCGTTTAAATAAATACATCAGCGAAAGCGGAATTTGCTCACGCCGCGAAGCGGATCGCTA
TATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAG
TAAACGGTCAGTTGATTGAGCCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTAGGCATTGTCAGC
ACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCGTGTTCCCGATTGGCCGCCTGGA
TAAAGACTCCCAGGGGCTGATTTTCCTCACCAATCACGGCGATCTGGTGAATAAGATCCTCCGTGCGGGTAACGATCATG
AGAAAGAGTATCTGGTGACGGTCGATAAACCGATTACCGATGAATTTATTCGTGGTATGGGGGCGGGGGTGCCGATCCTC
GGGACGGTGACTAAAAAGTGCAAAGTCAAAAAAGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCG
TCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGTACGCGCATTATGAACGTCAGCTTAA
GCGGCATTCCGCTGGGGGAGTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATCGAAAATTCCTCT
TCCGAGGCAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGC
GGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGAAAAAAGAAGGGGCGCTAA

Upstream 100 bases:

>100_bases
TAAAAGGCGTGGCAGATCACATTTAATTTCGACGTTGCCGCATAACCCTGTTTTTCCCCGCTAAGAGGTACGGTATATTC
CGCGTTTTACAGGAGAATTT

Downstream 100 bases:

>100_bases
TCAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTGCTTTTTCT
TTAACTGGCGGGCTTTTTTC

Product: 23S rRNA pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGDQVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIENSS
SEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKKKGR

Sequences:

>Translated_290_residues
MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGDQVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIENSS
SEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKKKGR
>Mature_290_residues
MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGDQVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVS
TTEDGERDNIVDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIENSS
SEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKKKGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790453, Length=290, Percent_Identity=98.9655172413793, Blast_Score=582, Evalue=1e-168,
Organism=Escherichia coli, GI1787524, Length=283, Percent_Identity=31.4487632508834, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=28.3842794759825, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUF_ECO57 (Q8X4L9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86095
- PIR:   E91254
- RefSeq:   NP_290656.1
- RefSeq:   NP_313032.1
- ProteinModelPortal:   Q8X4L9
- SMR:   Q8X4L9
- EnsemblBacteria:   EBESCT00000027623
- EnsemblBacteria:   EBESCT00000057326
- GeneID:   914336
- GeneID:   960101
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5620
- KEGG:   ecs:ECs5005
- GeneTree:   EBGT00050000009689
- HOGENOM:   HBG754478
- OMA:   MNVSLKG
- ProtClustDB:   PRK10475
- BioCyc:   ECOL83334:ECS5005-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 32405; Mature: 32405

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGDQVKPGDVVKVNGQLIE
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECCEEEC
PREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPILGTVTKKCKVKKEAPFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIENSS
HHHHCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKKKGR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGDQVKPGDVVKVNGQLIE
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECCEEEC
PREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPILGTVTKKCKVKKEAPFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDELIDLFKLIENSS
HHHHCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKKKGR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796