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The map label for this gene is yliF

Identifier: 209400162

GI number: 209400162

Start: 1003119

End: 1004360

Strand: Direct

Name: yliF

Synonym: ECH74115_0985

Alternate gene names: 209400162

Gene position: 1003119-1004360 (Clockwise)

Preceding gene: 209398618

Following gene: 209398000

Centisome position: 18.0

GC content: 43.8

Gene sequence:

>1242_bases
ATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTATTGATACTGCTTTTGCGGTGGCAAATATTGCTGAATATCG
GCGTAGCGTGGCAATTGATCTTATCAACACGCTAAATCCCACGGAGGAACAGCTGTTGGTTGGCTTACGCACAGCTTACG
CCGACTCGGTTTCACCCTCTTATTTGTACGATGTCGGTCCTTATCTGATTTCCAGTGACGAATGTATTCAGGTAAAGGAG
TTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGTCAAAAATACAGGGTTTATCTCTTTTGA
CGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATATTTTTGCTTGGTCTGGAGCGTTTTT
CTTTACTGTCAAAATCGCTGGCGATGGACAGCGAGAACCTGATGTTCTCTCTGTTTAAGAACGGTAAACCGGTGACCGGT
GATGAATATAATGCTAAAAACGCCATCTTCACCGTTTCGGAAGCGATGGAGCACTTCGCCTATTTGCCGACCGGATTGTA
CGTATTTGCGTATAAAAAAGATGTTTATTTGCGGGTCTGTACATTGATTATTTTCTTTGCCGCATTGGTGGCAGTGATAT
CGGGTGCCAGTTGCCTCTATCTGGTACGCAGAGTGATTAATCGTGGTATTGTGGAGAAAGAAGCCATTATTAATAACCAT
TTTGAACGCGTACTGGATGGCGGGCTTTTCTTTTCGGCTGCCGATGTCAAAAAACTCTACAGTATGTATAACTCGGCGTT
CCTGGACGACCTGACCAAAGCAATGGGTAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGAAAAAGGCGGTTATT
TGTGCCTGTTTGACGTCGATAAATTCAAAAATATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAGTGTTGATGAAA
GTGGTGAAAATCCTTAAATCACAGATCCCGGTAGATAAAGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGAT
TTATACTGGCGGCACGCTGGAAGAGTTACTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAA
GCACCAGTATCGGTGTAGCGCATTCAAATGAATGTACTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTAT
AAGAGTAAGAAAAACGGCAGGGCTCAGATTAGCTGGCAGTAG

Upstream 100 bases:

>100_bases
GAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTG
CCTGCGGGATCTACACTCAA

Downstream 100 bases:

>100_bases
AATTAGAATAGTGTTAGTACCCCTGAATCAAGGGGTACTAATGAATGTGGTAAATCATTTGCAAACATTTTACTTTGCAA
AAACTTCCGGCCCTTTTATT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MVKERVYSLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSDECIQVKE
FEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTG
DEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLY
KSKKNGRAQISWQ

Sequences:

>Translated_413_residues
MVKERVYSLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSDECIQVKE
FEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTG
DEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLY
KSKKNGRAQISWQ
>Mature_413_residues
MVKERVYSLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSDECIQVKE
FEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTG
DEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLY
KSKKNGRAQISWQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI1787056, Length=413, Percent_Identity=99.7578692493947, Blast_Score=844, Evalue=0.0,
Organism=Escherichia coli, GI1787816, Length=191, Percent_Identity=32.4607329842932, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.1851851851852, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=32.5153374233129, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=32.1637426900585, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=156, Percent_Identity=32.0512820512821, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=36.986301369863, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787262, Length=245, Percent_Identity=28.1632653061224, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=33.1460674157303, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YLIF_ECO57 (Q8X6V3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90743
- PIR:   D85593
- RefSeq:   NP_286598.1
- RefSeq:   NP_308940.1
- ProteinModelPortal:   Q8X6V3
- SMR:   Q8X6V3
- EnsemblBacteria:   EBESCT00000027893
- EnsemblBacteria:   EBESCT00000059993
- GeneID:   917650
- GeneID:   958134
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1058
- KEGG:   ecs:ECs0913
- GeneTree:   EBGT00050000008776
- HOGENOM:   HBG467595
- OMA:   SLYNSAF
- ProtClustDB:   CLSK879781
- BioCyc:   ECOL83334:ECS0913-MONOMER
- InterPro:   IPR001054
- InterPro:   IPR000160
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 46663; Mature: 46663

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x23a04448)-; HASH(0x25bcf998)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERVYSLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
YLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTH
HHEECCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEEEC
NRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFA
CCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
YLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVD
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEHH
KFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSIL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHH
KEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEH
>Mature Secondary Structure
MVKERVYSLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
YLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTH
HHEECCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEEEC
NRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFA
CCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
YLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVD
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEHH
KFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSIL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHH
KEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796