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The map label for this gene is yliE [H]

Identifier: 209398618

GI number: 209398618

Start: 1000676

End: 1003024

Strand: Direct

Name: yliE [H]

Synonym: ECH74115_0984

Alternate gene names: 209398618

Gene position: 1000676-1003024 (Clockwise)

Preceding gene: 209398764

Following gene: 209400162

Centisome position: 17.96

GC content: 41.63

Gene sequence:

>2349_bases
ATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGCCTTTT
TTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACG
TTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACTGTATTTATTAAAAAATACAGAGGAG
ATTCAACGCGCTGTGATCCTGAAAATGGAAATGATGCCATTTTTAGACTCGGTGGGACTGGTACTTGATGATAATAAATA
TTATCTCTTTTCGCGGAGGGCGAACGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGT
CAGGGCAGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGG
AATCCGGCCTACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACGCATCAACGGCAAAGATCACGA
TTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGAACGAGTATCTTGATCAAATCAGCGCTAATG
ATGAAGTTCTATTTTTGAAACAAGGCCATGAGATCATTGCCAAGAATCAACTCGCTCGTGAAAAACTGATTATTTATAAT
AGCGAAGGTGATTATAATATTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAATAACGCATT
ATTCGAAATCTATTTTTATTATCCGGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCA
TTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTTCCGTCGGCAATTTTCTGAAATGACAGAGCTGATT
AATACGCTGGCGTTTTTGCCTGACTCAACGGATCAGATCGAGGCTCTGAAAATTCGCGAAGGCGATGCGAAAGAGATTAT
CAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGGTCAAATAAATTGCTCTCACTGATCTCTTACG
ATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCCAACAATAATCAGTATCTGGCTGTGGGGATCATCAAA
CTGTGTGGTCTGGAAGCCGTGGAAGCTGTGTTTGGTGTTGATGAACGCAATAAAATCGTCAGGAAATTGTGTCAGCGAAT
TGCTGAGAAATATGCACAATGCTGCGATATCGTGACATTCAATGCCGATCTCTATTTACTTCTGTGTCGGGAAAATGTAC
AGACATTTACCCGTAAAATAGCGATGGTAAACGATTTTGACAGCAGCTTTGGCTACCGCAATCTGCGCATCCATAAGTCT
GCCATTTGTGAACCTTTGCAGGGGGAAAACGCCTGGAGTTACGCAGAAAAACTGAAACTGGCGATTTCCAGTATCCGTGA
CCATATGTTCTCCGAGTTTATTTTCTGTGATGACGCGAAACTCAACGAAATAGAAGAGAATATCTGGATTGCGCGTAATA
TTCGCCATGCAATGGAAATTGGCGAACTATTCCTCGTCTATCAACCGATCGTTGATATTAACACCCGCGCCATTCTGGGC
GCGGAGGCGTTGTGCCGTTGGGTGTCTGCGGAGCGGGGGATCATTTCACCGCTGAAGTTCATTACCATTGCTGAAGATAT
CGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGACATTTTAGTCAGCGTGCATCGC
TGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCGCACTTTCATGAGCGTTTTACCACC
ATCATGAAAGAAAATGGCCTGAAGGCGAACAGCCTCTGTGTTGAGATCACTGAAACCGTGATCGAGCGAATTAATGAACA
TTTTTATCTCAATATTGAACAACTGCGTAAACAAGGGTTACGGATATCGATTGATGACTTTGGCACCGGTTTGTCAAACC
TGAAACGTTTTTATGAAATTAATCCAGACAGCATAAAGGTGGACTCGCAATTTACCGGCGATATTTTCGGTACTGCGGGA
AAAATTGTGCGCATTATTTTCGACCTGGCACGCTATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGT
TGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTATCAGAAACCCATGCCATTCTCCGCCTGGG
ATAAAAGTGGAAAATTAGTAAAAGAGTAG

Upstream 100 bases:

>100_bases
TGATTTTGTAGAATCGGGTAATTTGGTTAAATAGCCGCAGCAAGGGACTGTTTTTGCAGCGGCACAGCGTTCAGACAGTT
ATTTTGTTAAATGTATTAAC

Downstream 100 bases:

>100_bases
TTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCG
GGATCTACACTCAAATGGTA

Product: cyclic diguanylate phosphodiesterase (EAL) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEE
IQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
SEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELI
NTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIK
LCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILG
AEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTT
IMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE

Sequences:

>Translated_782_residues
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEE
IQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
SEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELI
NTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIK
LCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILG
AEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTT
IMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE
>Mature_782_residues
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEE
IQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
SEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELI
NTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIK
LCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILG
AEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTT
IMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE

Specific function: Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787055, Length=782, Percent_Identity=99.3606138107417, Blast_Score=1594, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=294, Percent_Identity=30.2721088435374, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI87081921, Length=275, Percent_Identity=30.1818181818182, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1790496, Length=254, Percent_Identity=31.1023622047244, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87082096, Length=512, Percent_Identity=24.0234375, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081845, Length=239, Percent_Identity=29.2887029288703, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081980, Length=254, Percent_Identity=29.9212598425197, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788849, Length=250, Percent_Identity=26.8, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1786507, Length=249, Percent_Identity=29.3172690763052, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI226510982, Length=267, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI87081743, Length=241, Percent_Identity=26.9709543568465, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788502, Length=251, Percent_Identity=28.6852589641434, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 90070; Mature: 90070

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVV
HHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECCCCEEEE
YHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLR
EEHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
INGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
ECCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEE
SEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
CCCCEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCEECCCCCEEEHHHHHHHHHHHH
VYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIV
HHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHCCCHHHHHH
RKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
HHHHHHHHHHHHHHHCEEEECCCEEEEEEHHHHHHHHHHHHHEECCCCCCCCCEEEEEHH
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEI
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHH
GELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKT
HHHHEEEECCCCCCCHHHHCHHHHHHHHHCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHH
AMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
HHHCCCCEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCCCCCEEECCCCCCCHHHHHH
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
HHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
KE
CC
>Mature Secondary Structure
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVV
HHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECCCCEEEE
YHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLR
EEHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
INGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
ECCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEE
SEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
CCCCEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCEECCCCCEEEHHHHHHHHHHHH
VYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIV
HHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHCCCHHHHHH
RKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
HHHHHHHHHHHHHHHCEEEECCCEEEEEEHHHHHHHHHHHHHEECCCCCCCCCEEEEEHH
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEI
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHH
GELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKT
HHHHEEEECCCCCCCHHHHCHHHHHHHHHCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHH
AMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
HHHCCCCEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCCCCCEEECCCCCCCHHHHHH
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
HHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]