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The map label for this gene is opdE [H]

Identifier: 209400131

GI number: 209400131

Start: 332686

End: 333879

Strand: Direct

Name: opdE [H]

Synonym: ECH74115_0328

Alternate gene names: 209400131

Gene position: 332686-333879 (Clockwise)

Preceding gene: 209396830

Following gene: 209399769

Centisome position: 5.97

GC content: 57.12

Gene sequence:

>1194_bases
ATGTCGACACTAAGCCAAACACCATCACCTCATCATCAGCACGCATACTGGGGCGGTATTTTTGCCATGACCCTGTGCGT
GTTTGTGCTGATTGCTTCTGAATTTATGCCCGTCAGCCTGCTCACGCCGATTGCCCGTGACCTGGGCGTGACGGAGGGGC
TGGCAGGGCGGGGGATTGCTATCTCCGGTGCGCTGGCGGTTCTGACCAGCCTGACGCTTTCAACGCTAGCTGGAAAGATG
AATCGTAAGTTCCTGCTGCTGGGGATGACGGTTCTGATGGCCGTATCGGGGCTTATTATTGCGCTGGCTACCAGTTATCT
GATGTATATGGTCGGTCGTGCGATGATTGGTGTGGCCATTGGCGGATTCTGGTCGATGTCAGCGGCAACGGCGATTCGTC
TGGTGCCGCAGCATCAGGTCACGCGTGCGCTGGCGATTTTCAACGCCGGTAATGCGCTGGCGACGGTCGTGGCCGCGCCG
CTGGGAAGCTATCTGGGGGCTACGGTCGGATGGCGAGGCGCTTTCTTGTGCTTGGTGCCCATGGCGGTTGTCGCCTTTAT
CTGGCAGTGCATTAGTCTGCCCAGCATGGATGCGAATAAAAGCCACACGTCATGTGGCGCGGTATTCCGCTTATTTAGCC
GCCGCATGGTTGTGGTCGGCATGATAGCTTGTGGCCTGTTTTTCATGGGGCAGTTTGCGCTATTCACCTATGTGCGCCCA
TTTCTGGAGTCGGTGACACGGGTTAATTCCTTAGGCTTGTCGTTGATTTTGCTGATTATCGGCGTGGCCGGTTTTATTGG
CACGCTGGTTGTTTCGATATTTCTCAACAGAAAATTCTACCCCACGCTGATGGCCATACCCGGACTGATGGCCGTCATTG
CCATCGGTCTGATGCTTACCGGGCATCACGTCTGGATGGTCTCGCTGCTGTTAGGACTCTGGGGCATGCTGGCCACCGCC
GCGCCAACCGGATGGTGGACATGGATTGCGCGTACGTTGCCTGACAACGCCGAAGCCGGAGGCGGGCTCATGGTCGCGGT
GATTCAGCTCTCAATCGCCCTTGGATCAACGGCAGGAGGCATCGTGTTTGACCGCCTTGGCTGGCAGAGTACGTTTGCCA
TGAGTAGTGTGCTGCTCCTTTGCGCGGGCGTACTGACCTTTGTTACAGCACGCCAGAAGGCCGGTGCGCTTTAG

Upstream 100 bases:

>100_bases
ATTTTTCCAGACCAAACTGAAATAAAACCTCCCCTGAATTGATGCAAAGCCACCGGTCTATGGCCGGTGGCTTTTATCCT
GATCTTTGTGAAATTTCACT

Downstream 100 bases:

>100_bases
TCCGGCGAGGGCGCGCGTTGCTGAAAAAGTTACACAAGGCGATGAGCAACCACTATGGGTAGGGCGCTCTTTTAAACGAT
TTAGGTGATTTTCTAAAACG

Product: purine ribonucleoside efflux pump NepI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSTLSQTPSPHHQHAYWGGIFAMTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALAVLTSLTLSTLAGKM
NRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFWSMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAP
LGSYLGATVGWRGAFLCLVPMAVVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLMLTGHHVWMVSLLLGLWGMLATA
APTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGSTAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL

Sequences:

>Translated_397_residues
MSTLSQTPSPHHQHAYWGGIFAMTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALAVLTSLTLSTLAGKM
NRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFWSMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAP
LGSYLGATVGWRGAFLCLVPMAVVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLMLTGHHVWMVSLLLGLWGMLATA
APTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGSTAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL
>Mature_396_residues
STLSQTPSPHHQHAYWGGIFAMTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALAVLTSLTLSTLAGKMN
RKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFWSMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPL
GSYLGATVGWRGAFLCLVPMAVVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRPF
LESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLMLTGHHVWMVSLLLGLWGMLATAA
PTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGSTAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL

Specific function: Regulates the expression of oprD which encodes the imipenem-specific porin [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywfA [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=377, Percent_Identity=39.7877984084881, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1787947, Length=298, Percent_Identity=25.8389261744966, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787808, Length=229, Percent_Identity=25.3275109170306, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786595, Length=348, Percent_Identity=24.4252873563218, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41927; Mature: 41796

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STLSQTPSPHHQHAYWGGIFAMTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCH
ISGALAVLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFWSMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVP
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAVVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGSTAGG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
IVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1427017; 10984043 [H]