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The map label for this gene is mdtM

Identifier: 209399400

GI number: 209399400

Start: 5504027

End: 5505259

Strand: Reverse

Name: mdtM

Synonym: ECH74115_5851

Alternate gene names: 209399400

Gene position: 5505259-5504027 (Counterclockwise)

Preceding gene: 209397786

Following gene: 209400166

Centisome position: 98.8

GC content: 54.5

Gene sequence:

>1233_bases
ATGCCACGTTTTTTTGCCCGCCATGCCGCCACGCTGTTTTTCCCGATGGCGTTGATTTTGTATGACTTTGCCGCCTATCT
TTCGACGGACCTGATCCAGCCTGGGATCATTAATGTGGTCCGTGATTTTAATGCCGATGTCAGTCTGGCTCCGGCTGCCG
TCAGTCTCTATCTCGCTGGAGGCATGGCGTTGCAGTGGCTGCTGGGGCCGCTTTCCGACAGGATTGGCCGCAAGCCAGTG
CTGATTACCGGGGCGCTAATATTTACCCTTGCCTGCGCTGCGACAATGTTCACAACGTCTATGACACAGTTTCTTATCGC
GCGTGCAATTCAGGGCACCAGTATCTGTTTTATTGCCACCGTTGGTTATGTCACGGTGCAGGAGGCGTTCGGCCAGACAA
AAGGGATCAAGTTGATGGCGATTATCACCTCCATCGTACTGATTGCGCCGATTATCGGCCCGCTTTCCGGCGCAGCTCTG
ATGCACTTTGTGCACTGGAAAGTCCTTTTTGCCATCATTGCGGTTATGGGTTTTATCTCATTTGTTGGCTTACTGCTGGC
GATGCCAGAGACGGTGAAGCGCGGCGCGGTTCCATTTAGCGCCAAAAGCGTCTTGCGCGATTTTCGTAATGTCTTTTGCA
ATCGGCTGTTCCTCTTTGGCGCAGCAACCATCTCTTTAAGCTATATCCCCATGATGAGTTGGGTGGCTGTCTCGCCGGTG
ATCCTGATTGACGCAGGCGGCTTAACGACCTCGCAGTTCGCCTGGACACAGGTTCCGGTGTTCGGCGCGGTGATTGTCGC
GAATGCCATCGTGGCGCGTTTCGTTAAAGATCCGACCGAACCGCGCTTTATCTGGCGTGCCGTACCCATTCAACTGGTCG
GCCTCGCGCTGTTGATTATCGGCAATCTGCTGTCACCGCACGTCTGGCTGTGGTCGGTGCTGGGCACCAGTCTGTATGCT
TTCGGGATTGGTTTGATCTTCCCGACCTTATTCCGCTTTACGCTGTTTTCCAATAACTTACCGAAAGGGACCGTCTCCGC
ATCGCTGAATATGGTGATCCTGATGGTGATGTCTGTCTCGGTCGAAATTGGCCGCTGGCTGTGGTTTAACGGCGGTCGCT
TGCCGTTTCATCTGTTAGCCGTTGTGGCGGGCGTTATCGTCGTTTTCACCCTGGCCGGATTGCTTAATCGCGTGCGCCAA
CATCAGGCTGCCGAGCTGGCAGAGGAGCAGTGA

Upstream 100 bases:

>100_bases
AATTCAGTACATTTGTCCTTCTCGCCTACCTTTCGTCATTACCGACGGTATTGAATTTCGTTTTCCCCGTTGGGGTTCTC
CGGACAAGGAGTTGTTTGTT

Downstream 100 bases:

>100_bases
TTTTTGCGCGATCCTGCCGTCAGGCTCTATTCTTAACGTTATGAATAAACTCATTGAACTCAGACGCGCCAAAATGTTGG
CGCTCTCTTTACTGCTTATC

Product: multidrug resistance protein MdtM

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
ILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYA
FGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELAEEQ

Sequences:

>Translated_410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
ILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYA
FGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELAEEQ
>Mature_409_residues
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVL
ITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALM
HFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPVI
LIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAF
GIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQH
QAAELAEEQ

Specific function: Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1790794, Length=410, Percent_Identity=98.0487804878049, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1787065, Length=376, Percent_Identity=41.2234042553192, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1790146, Length=227, Percent_Identity=29.5154185022026, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI48994889, Length=223, Percent_Identity=27.8026905829596, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788509, Length=284, Percent_Identity=21.1267605633803, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082312, Length=184, Percent_Identity=25.5434782608696, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTM_ECO57 (Q8XB84)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91291
- PIR:   F86132
- RefSeq:   NP_290956.1
- RefSeq:   NP_313327.1
- ProteinModelPortal:   Q8XB84
- EnsemblBacteria:   EBESCT00000026564
- EnsemblBacteria:   EBESCT00000059649
- GeneID:   913623
- GeneID:   959712
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5939
- KEGG:   ecs:ECs5300
- GeneTree:   EBGT00050000008820
- HOGENOM:   HBG289858
- OMA:   PLMSWVA
- ProtClustDB:   PRK15403
- BioCyc:   ECOL83334:ECS5300-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 44524; Mature: 44393

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe4eb330)-; HASH(0x14069904)-; HASH(0x13e1bcfc)-; HASH(0x11d25964)-; HASH(0x1399ec28)-; HASH(0x13c8df9c)-; HASH(0x140735f0)-; HASH(0x122df41c)-; HASH(0x121fcb40)-; HASH(0x133ad6bc)-; HASH(0x14073368)-; HASH(0x14070a94)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH
GMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIAT
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHH
VGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFVHWKVLFAIIAVMGFIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
HHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLII
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVS
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQHQAAELAEEQ
HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH
GMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIAT
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHH
VGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFVHWKVLFAIIAVMGFIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
HHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLII
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVS
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQHQAAELAEEQ
HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796