Click here to switch to the map view.

The map label for this gene is lplA [H]

Identifier: 209399317

GI number: 209399317

Start: 5553524

End: 5555212

Strand: Reverse

Name: lplA [H]

Synonym: ECH74115_5901

Alternate gene names: 209399317

Gene position: 5555212-5553524 (Counterclockwise)

Preceding gene: 209400314

Following gene: 209399734

Centisome position: 99.7

GC content: 56.9

Gene sequence:

>1689_bases
ATGGCTCGCACAAAACTGAAATTCCGGCTGCATCGGGCAGTGATTGTCCTGTTCTGTCTTGCCTTGTTAGTGGCACTGAT
GCAGGGGGCGTCATGGTTTAGTCAAAACCACCAGCGACAGCGTAATCCACAGCTGGAAGAACTGGCCCGCACCCTGGCGC
GTCAGGTGACGCTGAACGTTGCACCGCTGATGCGTACCGACTCACCGGATGAAAAACGCATTCAGGCGATCCTCGATCAG
TTAACGGATGAAAGCCGTATCCTCGACGCGGGTGTGTATGACGAACAAGGCGATCTTATCGCACGTTCTGGCGAAAGCGT
CGAAGTGCGCGATCGGCTGGCGCTCGACGGTAAAAAAGCAGGCGGCTATTTTAACCAGCAGATTGTCGAGCCAATTGCCG
GGAAAAACGGACCGCTCGGCTATCTGCGCCTGACACTCGACACCCATACGCTCGCCACCGAAGCCCAACAGGTGGATAAC
ACCACTAACATTTTACGCCTGATGTTGCTGCTCTCACTGGCAATCGGCGTAGTGCTGACCCGCACGCTGCTACAGGGTAA
ACGCACCCGCTGGCAGCAATCGCCCTTCCTGTTAACCGCCAGCAAACCGGTGCCGGAAGAGGAAGAAAGCGAGAAAAAAG
AGTGCCCCATTACTACAAGAAAGGAAATCGTTATGTCCACATTACGCCTGCTCATCTCTGACTCTTACGACCCGTGGTTT
AACCTGGCGGTGGAAGAGTGTATTTTTCGCCAAATGCCCGCCACGCAGCGCGTTCTGTTTCTCTGGCGCAATGCCGACAC
GGTAGTAATTGGTCGCGCGCAGAACCCGTGGAAAGAGTGTAATACCCGGCGGATGGAAGAAGATAACGTCCGCCTGGCGC
GGCGCAGTAGCGGTGGCGGCGCGGTGTTCCACGATCTCGGCAATACCTGCTTTACCTTTATGGCTGGCAAGCCGGAGTAC
GATAAAACCATCTCCACGTCGATTGTGCTCAATGCACTGAATGCGCTCGGCGTCAGTGCCGAAGCGTCCGGACGTAACGA
TCTGGTGGTGAAAACCGCCGAAGGCGACCGCAAAGTCTCAGGATCGGCCTATCGCGAAACCAAAGATCGTGGCTTCCACC
ACGGCACCTTGCTGCTCAATGCCGACCTTAGCCGCCTGGCAAACTATCTCAATCCGGATAAAAAGAAACTGGCGGCGAAA
GGCATTACTTCAGTGCGTTCCCGCGTGACCAACCTCACCGAGCTGCTGCCGGGGATCCCCCATGAGCAGGTTTGCGAGGC
CATAACCGAGGCCTTTTTCGCCCATTATGGCGAGCGCGTGGAAGCGGAAATCATCTCCCCGGACAAAACGCCAGACTTGC
CAAACTTCGCCGAAACCTTTGCCCGCCAGAGTAGCTGGGAGTGGAACTTCGGTCAGGCTCCGGCATTCTCGCATCTGCTG
GATGAACGCTTTAGCTGGGGCGGCGTGGAACTGCATTTCGACGTTGAAAAAGGCCATATCACCCGCGCCCAGGTGTTTAC
CGACAGCCTCAACCCCGCGCCGCTGGAAGCCCTCGCCGGGCGACTGCAAGGCTGCCTGTACCGCGCGGATATGCTGCAAC
AAGAGTGCGAAGCGCTGTTGGTTGACTTCCCGGACCAGGAAAAAGAGCTACGGGAGTTGTCGACGTGGATAGCGGGGGCG
GTAAGGTAA

Upstream 100 bases:

>100_bases
CTCCTGTAAAATCGTTCGAAGCAGGGAAAATAACGCATGAGGCTACCTTGTATCCATTGCTTCTGGCAACATTAAGTCTC
AAATTTTCAAAGGGTGGAAG

Downstream 100 bases:

>100_bases
CTCGCTGATTAAAATATCCAAGGAAGGAAATATGATCGATCTGGAAAACCAGGAACGGGAGATTATCAATTTAATGTTCA
GCCAGCGTATTTCCTGGCTA

Product: unknown domain/lipoate-protein ligase A fusion protein

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQ
LTDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWF
NLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEY
DKTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAK
GITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLL
DERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIAGA
VR

Sequences:

>Translated_562_residues
MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQ
LTDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWF
NLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEY
DKTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAK
GITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLL
DERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIAGA
VR
>Mature_561_residues
ARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQL
TDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDNT
TNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWFN
LAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYD
KTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKG
ITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLD
ERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIAGAV
R

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=279, Percent_Identity=34.0501792114695, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21729884, Length=279, Percent_Identity=34.0501792114695, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21729882, Length=279, Percent_Identity=34.0501792114695, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21729880, Length=279, Percent_Identity=34.0501792114695, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21729878, Length=279, Percent_Identity=34.0501792114695, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1790846, Length=338, Percent_Identity=97.9289940828402, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1790847, Length=214, Percent_Identity=100, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71981909, Length=243, Percent_Identity=32.5102880658436, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322415, Length=292, Percent_Identity=28.0821917808219, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573464, Length=286, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 63086; Mature: 62954

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
APLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKA
HHHCCCCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCEEECCCCCEEHHHHHHCCCCCC
GGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDNTTNILRLMLLLSLAIGVVLT
CCCHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWF
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHH
NLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGG
HHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHCCCCCCCCCCEEEECCCCC
AVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVS
CHHHHCCCHHEEHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
GSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIP
CCHHHHHHHCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLL
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHH
DERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALL
HHHCCCCCEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VDFPDQEKELRELSTWIAGAVR
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
APLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKA
HHHCCCCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCEEECCCCCEEHHHHHHCCCCCC
GGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDNTTNILRLMLLLSLAIGVVLT
CCCHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWF
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHH
NLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGG
HHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHCCCCCCCCCCEEEECCCCC
AVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVS
CHHHHCCCHHEEHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
GSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIP
CCHHHHHHHCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLL
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHH
DERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALL
HHHCCCCCEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VDFPDQEKELRELSTWIAGAVR
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA