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The map label for this gene is rseP

Identifier: 209399122

GI number: 209399122

Start: 199871

End: 201223

Strand: Direct

Name: rseP

Synonym: ECH74115_0186

Alternate gene names: 209399122

Gene position: 199871-201223 (Clockwise)

Preceding gene: 209399460

Following gene: 209400867

Centisome position: 3.59

GC content: 50.85

Gene sequence:

>1353_bases
ATGCTGAGTTTTCTCTGGGATTTGGCTTCGTTCATCGTTGCACTGGGTGTACTTATCACCGTGCATGAATTTGGTCATTT
CTGGGTTGCCCGGCGTTGTGGTGTTCGCGTTGAGCGTTTCTCAATAGGGTTTGGTAAGGCGCTCTGGCGGCGAACTGATA
AGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTC
GTTCCGGAACTCCGCCACCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGC
AAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTACGTCCGGTGGTTGGCGAAA
TAGCAGCCAATTCGATAGCTGCGGAAGCACAAATTGCACCAGGTACGGAACTAAAAGCCGTAGATGGTATCGAAACGCCT
GATTGGGATGCCGTGCGTTTGCAGTTGGTCGATAAAATTGGCGATGAAAGCACCACCATTACAGTAGCGCCATTTGGCAG
CGACCAACGGCGGGATGTAAAGCTCGATTTACGTCACTGGGCGTTTGAGCCTGATAAAGAAGATCCGGTATCTTCGCTGG
GGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGAAAATGTGCAGCCAAACTCGGCGGCAAGCAAGGCAGGTTTG
CAAGCAGGCGACAGGATCGTTAAAGTCGATGGTCAGCCCTTAACGCAGTGGGTGACCTTTGTGATGCTTGTCCGGGATAA
CCCGGGTAAATCCTTAGCGTTAGAAATCGAAAGGCAGGGGAGTCCCTTGTCTTTGACATTAATCCCGGAGAGTAAACCGG
GTAATGGTAAAGCGATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAG
TATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAA
ATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAAC
TCGGGGTAGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTA
CTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAAGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTG
TTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGA

Upstream 100 bases:

>100_bases
ACGGTGGTATTTTAGATCGTATTGATAGCCTGACGGCTGCGGTACCGGTCTTTGCTTGCTTGTTGTTACTGGTATTCAGG
ACGCTTTAACGGAAGGTAAT

Downstream 100 bases:

>100_bases
GAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACC
GTATACGGTGCTGAAGGGTT

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
>Mature_450_residues
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPV
VPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ
YGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=100, Blast_Score=903, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSEP_ECO57 (P0AEH2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85502
- PIR:   B90651
- RefSeq:   NP_285870.1
- RefSeq:   NP_308205.1
- ProteinModelPortal:   P0AEH2
- EnsemblBacteria:   EBESCT00000027320
- EnsemblBacteria:   EBESCT00000060284
- GeneID:   913885
- GeneID:   956902
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0187
- KEGG:   ecs:ECs0178
- GeneTree:   EBGT00050000011030
- HOGENOM:   HBG664087
- OMA:   HGTEFVV
- ProtClustDB:   PRK10779
- BioCyc:   ECOL83334:ECS0178-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915
- SMART:   SM00228
- TIGRFAMs:   TIGR00054

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50; SSF50156 PDZ

EC number: NA

Molecular weight: Translated: 49072; Mature: 49072

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50106 PDZ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 23-23

Signals:

None

Transmembrane regions:

HASH(0x248a95b0)-; HASH(0x24033900)-; HASH(0x2447b544)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEE
IALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
TVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
EEEECCCCCCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCC
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIG
CCCCEEEEECCCCHHHHHHEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEE
FVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
EEECCCEEEECCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFCYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEE
IALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
TVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGL
EEEECCCCCCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCC
QAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIG
CCCCEEEEECCCCHHHHHHEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEE
FVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
EEECCCEEEECCCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFCYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796