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The map label for this gene is hsdR [H]

Identifier: 209398938

GI number: 209398938

Start: 5514767

End: 5517199

Strand: Reverse

Name: hsdR [H]

Synonym: ECH74115_5861

Alternate gene names: 209398938

Gene position: 5517199-5514767 (Counterclockwise)

Preceding gene: 209399880

Following gene: 209398363

Centisome position: 99.02

GC content: 49.45

Gene sequence:

>2433_bases
ATGGCGGAACTGAACCTAAGTAACCTGACGGAAGCAGACATCATTACCAAATGCGTTATGCCAGCCATTCTCAATGCAGG
CTGGGACAACACAACGCAAATCAGACAGGAGGTCAAACTCCGGGACGGTAAAGTCATCGTACGTGGTAAAGTTGCGGCAC
GCAGAACGGTAAAATCTGCTGACATCGTGCTGTATCACAAACCTGGCATTCCCTTAGCCGTGATTGAAGCAAAAGCCAAC
AAACATGAAATTGGCAAAGGGATGCAACAGGGCATTGAATATGCGCGCCTGCTGGACGTTCCTTTTGTTTTTGCCACCAA
CGGTGATGGCTTTATCTTCCGCGATGCCACCGCAGCCGAAGGTGAATGCCTTGAAAAGCAAATAACGCTGGATGACTTCC
CCTCCCCCGCTGAACTCTGGCAAAAATTCTGCCTCTGGAAAGGATACACACAAGCTCAGCTTCCGGTGATTACTCAGGAC
TATTACGACGATGGCAGCGGTAAATCGCCACGTTATTACCAGCTTCAGGCAATCAACAAAACCATTGAAGCCGTCTCCAA
CGGGCAAAACCGCGTTCTGCTGGTCATGGCGACCGGAACAGGGAAAACCTATACCGCATTCCAGATCATCTGGCGCCTGT
GGAAATCAAAAAATAAAAAACGCATTTTGTTCCTTGCCGATCGCAATATTCTGGTCGACCAAACCAAAAATAATGATTTC
CAGCCATTTGGTACGGCAATGACCAAAGTCAGCGGACGCACCATTGATCCCGCTTATGAAATTCACCTCGCGCTCTATCA
GGCTATAACTGGCCCGGAGGAAGACCAAAAAGCGTTTAAACAAGTCGCACCAGATTTCTTCGATCTGATCGTGATCGACG
AATGCCATCGCGGCAGCGCATCCGAAGACAGCGCCTGGCGAGAAATCCTTGATTATTTTAGTTCCGCCACCCAAATTGGC
TTAACCGCCACGCCAAAAGAGACGCATGAAGTCTCCAGCACGGATTACTTCGGCGATCCGGTTTACGTCTACTCACTAAA
AGAAGGGATCGAAGACGGCTTCCTCGCCCCTTATAAAGTTGTCCGTGTTGATATTGATGTTGATCTGCAAGGCTGGCGCC
CAACCAAAGGGCAAACTGACTTAAACGGCGAAGTGATCGACGATCGTATCTATAACCAGAAAGATTTCGATCGCACGATG
GTAATCGACGAACGCACAGAACTGGTTGCCAGAACCATTACCGACTACCTCAAGCGTACCAATCCGATGGATAAAACCAT
CGTCTTCTGTAACGACATCGATCATGCAGAACGTATGCGCCGCGCCCTGGTTAATCTCAACCCGGAGCAGGTGAAAAAGA
ACGACAAATACGTCATGAAAATCACCGGCGATGATGAAATTGGCAAAGCTCAGTTGGATAACTTCATCAACCCGAAAAAA
CCGTACCCGGTTATCGCGACCACTTCAGAGCTGATGACCACCGGTGTGGATGCTAAAACCTGCAAACTGGTAGTACTGGA
CCAGAACATCCAGTCGATGACCAAATTCAAGCAGATTATCGGTCGTGGTACACGCATCGACGAACGTTACGGCAAACTCT
GGTTTACCATCCTCGACTTTAAAAAAGCCACCGAACTGTTTGCCGATGAGCGTTTCGATGGCATTCCCGAAAAAGTCATG
GATACCACACCAGAGGATATCGCCGATCCAGAATCTGATTTTGAAGAGAAACTCGAAGAAATCAGCGAACATGACGACGA
ACAGGTAACAGGCGTTGATGAACCGCCTGCGCCACCATACCAGGTTAAAGATACCGATGATGTCGGCCCACTTCCGGAAG
AAGACGAGAAGAAAATCCGCAAGTTTCACGTCAACGGTGTAGCAGTGGGCGTTATTGCCCAGCGTGTTCAGTATTACGAC
GCCGACGGTAAACTGGTTACCGAATCCTTTAAAGATTACACCCGCAAAACACTGCTCAAAGAATATGCCTCGCTGGATGA
CTTTACCCGCAAGTGGCAGGACGCCGATCGCAAAGAAGCGATCATTCACGAGCTGGAGCAACAGGGGATCATCTGGGAAG
TACTGGCAGAAGAAGTCGGTAAAGATCTCGACCCGTTCGACATGCTTTGCCACGTAGTGTATGGTCAGCCGCCGTTAACC
CGCAAAGAGCGCGCCGAGAACGTGCGCAAGCGGAACTACTTCACAAAATACTCTGAAGCAGCGCAAGCCGTGCTCGATAA
TCTGCTGGATAAATACGCCGATGCGGGCGTACAGGAGATCGAAAGTATTCAGGTGCTGAAACTTAAGCCATTCGACAGCA
TGGGCACCTTACCGGAGATTATTAAAACCGGATTTGGCGACCGTAACGGGTATAATCAGGCGCTCAGCGAGCTGGAAAAC
GAAATCTACCAATTACCGCCCCGCTCTGCTTAA

Upstream 100 bases:

>100_bases
CTCTGCTACGTACTTTACGTTGAAACACCGCCCTAAATCATAGCCAGGCTATTGATTTTATGTACAATCAGAATCGTCCT
GATAAAAGCGAGAATACCCC

Downstream 100 bases:

>100_bases
CAACTCTCTTTTGCGCCTTCTGCAACCATCATGTTGCAGAAGGCCTTGTTTTTCATGGAATACAACATGTCTATCAGCTC
AGTAATCAAATCATTACAAG

Product: type III restriction enzyme domain protein

Products: NA

Alternate protein names: R.EcoAI [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKAN
KHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDF
QPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIG
LTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTM
VIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVM
DTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYD
ADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLT
RKERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELEN
EIYQLPPRSA

Sequences:

>Translated_810_residues
MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKAN
KHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDF
QPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIG
LTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTM
VIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVM
DTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYD
ADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLT
RKERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELEN
EIYQLPPRSA
>Mature_809_residues
AELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANK
HEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDY
YDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQ
PFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGL
TATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMV
IDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKKP
YPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMD
TTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYDA
DGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTR
KERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELENE
IYQLPPRSA

Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=635, Percent_Identity=26.6141732283465, Blast_Score=163, Evalue=5e-41,
Organism=Escherichia coli, GI1788511, Length=424, Percent_Identity=24.0566037735849, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 92085; Mature: 91954

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHCCC
DIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAE
CEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCC
GECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINK
CHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEHHHHHH
TIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDF
HHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCC
QPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
SEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKV
CCHHHHHHHHHHHHHHHHCCEECCCCHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCEEE
VRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRT
EEEEEEEECCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHC
NPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
CCCCCEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCC
PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDF
CCCEEEEHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPY
HHHHHHHHHHHCCCCCHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
QVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLK
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
EYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
RKERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCCCCCHHHH
IKTGFGDRNGYNQALSELENEIYQLPPRSA
HHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHCCC
DIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAE
CEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCC
GECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINK
CHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEHHHHHH
TIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDF
HHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCC
QPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
SEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKV
CCHHHHHHHHHHHHHHHHCCEECCCCHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCEEE
VRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRT
EEEEEEEECCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHC
NPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
CCCCCEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCC
PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDF
CCCEEEEHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPY
HHHHHHHHHHHCCCCCHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
QVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLK
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
EYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
RKERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCCCCCHHHH
IKTGFGDRNGYNQALSELENEIYQLPPRSA
HHCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]