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The map label for this gene is thiL

Identifier: 209398517

GI number: 209398517

Start: 503475

End: 504452

Strand: Direct

Name: thiL

Synonym: ECH74115_0499

Alternate gene names: 209398517

Gene position: 503475-504452 (Clockwise)

Preceding gene: 209396859

Following gene: 209400639

Centisome position: 9.04

GC content: 54.7

Gene sequence:

>978_bases
ATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCAT
CGGCGACGATTGCGCTCTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGCAACC
ATTTCCTCCCTGATATCGATCCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGG
GCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGACAGTTT
GTTTGATCTTCTCAATTATTACGATATGCAACTCATTGGCGGCGATACCACGCGTGGGCCATTATCAATGACGTTGGGTA
TCCACGGCTTTGTTCCGATGGGACGAGCCTTAACGCGCTCTGGGGCGAAACCGGGTGACTGGATCTATGTGACCGGTACA
CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGAT
CAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCTATCGATCTCT
CTGACGGTCTGATTTCCGATCTCGGGCATATCGTGAAAGCCAGCGACTGCGGCGCACGTATTGACCTGGCATTGCTGCCG
TTTTCTGATGCGCTTTCTCGCCATGTTGTACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTT
GTGTTTCACGGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGTCATCTGGGCGTACCGTTTACCTGTATCG
GGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTCACGTTAGACTGGAAAGGATATGAC
CATTTTGCCACGCCATAA

Upstream 100 bases:

>100_bases
TGATTCGCCCTAACAAAAAGTAATATCCAGGCCGGTAGATTCACGGAAGACTGTTCCGCGATCGCCGGCCTTTTCTTTTT
TACCTGCTGAGGCATAACGT

Downstream 100 bases:

>100_bases
AGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTGCTTGCTGTCGGATTCGGAAGTGGATTAAGCCCAA
TCGTTCCTGGCACGATGGGA

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
FSDALSRHVVPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYD
HFATP

Sequences:

>Translated_325_residues
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
FSDALSRHVVPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYD
HFATP
>Mature_324_residues
ACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGA
DPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTP
GDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLPF
SDALSRHVVPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDH
FATP

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family

Homologues:

Organism=Escherichia coli, GI1786619, Length=325, Percent_Identity=99.6923076923077, Blast_Score=652, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_ECO57 (Q8XE87)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85538
- PIR:   F90687
- RefSeq:   NP_286158.1
- RefSeq:   NP_308497.1
- ProteinModelPortal:   Q8XE87
- SMR:   Q8XE87
- EnsemblBacteria:   EBESCT00000026029
- EnsemblBacteria:   EBESCT00000057957
- GeneID:   914572
- GeneID:   957378
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0519
- KEGG:   ecs:ECs0470
- GeneTree:   EBGT00050000011174
- HOGENOM:   HBG577902
- OMA:   MGAEPAW
- ProtClustDB:   PRK05731
- BioCyc:   ECOL83334:ECS0470-MONOMER
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283
- PIRSF:   PIRSF005303
- TIGRFAMs:   TIGR01379

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =2.7.4.16

Molecular weight: Translated: 35041; Mature: 34910

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIG
HHHHHHHHHEECHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTPGDSAAGLAILQNRLQVADA
CCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
KDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
CCHHHHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCEEEEEEEC
FSDALSRHVVPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADI
CHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHCCC
EGLCFIRDGEPVTLDWKGYDHFATP
CEEEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIG
HHHHHHHHHEECHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTPGDSAAGLAILQNRLQVADA
CCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
KDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
CCHHHHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCEEEEEEEC
FSDALSRHVVPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADI
CHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHCCC
EGLCFIRDGEPVTLDWKGYDHFATP
CEEEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796