BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is yvgN [H]

Identifier: 209398421

GI number: 209398421

Start: 356670

End: 357245

Strand: Reverse

Name: yvgN [H]

Synonym: ECH74115_0350

Alternate gene names: 209398421

Gene position: 357245-356670 (Counterclockwise)

Preceding gene: 209400009

Following gene: 209396166

Centisome position: 6.41

GC content: 50.87

Gene sequence:

>576_bases
ATGCAAAAAGTAAAACTGAATAACGGTATTGAAATGCCCCTGCTGGGCTTTGGTGTATTTCAGATGACCGATGCCGCCGA
ATGCGAAAGAGCGGTCATTGACGCTATTAACACCGGTTATCGACTGATTGACACCGCCGCCTCCTATCAAAATGAACCCC
AGGTCGGGAACGCACTGAAACAAAGCGGTATCGCCCGTAACGAACTCTTTGTCACCACGAAACTCTGGTTGCAGGATACC
AGTTACGAAGGGGCCAAAGCCCAGTTCGAACGCTCTCTGAATCGACTGCAACTTGATTACGTTGACCTGTATTTGATTCA
TCAGCCTTACGGTGATGTCCACGGTGCCTGGCGTGCCATGGAAGAGCTGCAACAGGCCGGTAAAATTCGCGCTATTGGCG
TCAGTAACTTCCATCCTGACCGACTGGCTGACCTTATCGCCTTTAATAACGTCGTTCCAGCGGTAAACCAGGTTGAAGTT
AACCCTTTCAATCAGCAGCTACACGCCGTTCCGTGGATGCAAAGCCGTGGTATTCAGCCAGAAGCCTGGGCACCGTTTGC
AGTTACAGGGCGGTAA

Upstream 100 bases:

>100_bases
TTTATGAATTTCGTTCATAACCCCTGACAAATCCTGCCGTCTAATCGAGCTCTTCTCTTTACGTTATGCTTCTCATGAAC
GATAACGGAAGGATAACACC

Downstream 100 bases:

>100_bases
GCGATTATGCATCTACCTATTTACGTCGGTCACTCAGTCAGAGTCTTGCCCATATTCAATGAGGTTCAACACCGCAGCCA
ATGAAAAGTAGGCCATGCGG

Product: morphine 6-dehydrogenase

Products: NA

Alternate protein names: GR; Methylglyoxal reductase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLIDTAASYQNEPQVGNALKQSGIARNELFVTTKLWLQDT
SYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
NPFNQQLHAVPWMQSRGIQPEAWAPFAVTGR

Sequences:

>Translated_191_residues
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLIDTAASYQNEPQVGNALKQSGIARNELFVTTKLWLQDT
SYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
NPFNQQLHAVPWMQSRGIQPEAWAPFAVTGR
>Mature_191_residues
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLIDTAASYQNEPQVGNALKQSGIARNELFVTTKLWLQDT
SYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
NPFNQQLHAVPWMQSRGIQPEAWAPFAVTGR

Specific function: Reduces glyoxal and methylglyoxal. Is not involved in the vitamin B6 biosynthesis [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=214, Percent_Identity=35.0467289719626, Blast_Score=132, Evalue=1e-31,
Organism=Homo sapiens, GI5174391, Length=214, Percent_Identity=35.0467289719626, Blast_Score=132, Evalue=1e-31,
Organism=Homo sapiens, GI310109922, Length=192, Percent_Identity=39.0625, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI310109920, Length=207, Percent_Identity=36.7149758454106, Blast_Score=118, Evalue=3e-27,
Organism=Homo sapiens, GI45446745, Length=213, Percent_Identity=36.150234741784, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI4503285, Length=213, Percent_Identity=36.150234741784, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI5453543, Length=206, Percent_Identity=35.4368932038835, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI223468663, Length=215, Percent_Identity=33.0232558139535, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI300116273, Length=218, Percent_Identity=31.651376146789, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI24497583, Length=206, Percent_Identity=33.9805825242718, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI5174695, Length=218, Percent_Identity=30.7339449541284, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI24497585, Length=207, Percent_Identity=34.2995169082126, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI4502049, Length=216, Percent_Identity=35.1851851851852, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI291291012, Length=193, Percent_Identity=31.6062176165803, Blast_Score=98, Evalue=4e-21,
Organism=Homo sapiens, GI93277124, Length=223, Percent_Identity=28.6995515695067, Blast_Score=95, Evalue=3e-20,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=44.7368421052632, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=44.7368421052632, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI300116271, Length=195, Percent_Identity=29.7435897435897, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI87082198, Length=191, Percent_Identity=43.979057591623, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786400, Length=183, Percent_Identity=36.6120218579235, Blast_Score=116, Evalue=8e-28,
Organism=Escherichia coli, GI1788081, Length=203, Percent_Identity=30.5418719211823, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI48994888, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17564128, Length=219, Percent_Identity=36.5296803652968, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17550248, Length=203, Percent_Identity=35.9605911330049, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71998625, Length=192, Percent_Identity=36.9791666666667, Blast_Score=123, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17552492, Length=192, Percent_Identity=35.9375, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17561298, Length=202, Percent_Identity=32.6732673267327, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17537077, Length=215, Percent_Identity=34.8837209302326, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537075, Length=215, Percent_Identity=34.8837209302326, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562292, Length=178, Percent_Identity=33.7078651685393, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17561300, Length=204, Percent_Identity=34.8039215686275, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17566692, Length=201, Percent_Identity=34.3283582089552, Blast_Score=109, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17537079, Length=217, Percent_Identity=31.7972350230415, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538386, Length=201, Percent_Identity=30.8457711442786, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324694, Length=217, Percent_Identity=39.1705069124424, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320576, Length=215, Percent_Identity=39.0697674418605, Blast_Score=142, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321896, Length=219, Percent_Identity=32.4200913242009, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322556, Length=198, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319625, Length=223, Percent_Identity=33.1838565022422, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320079, Length=203, Percent_Identity=33.4975369458128, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663317, Length=211, Percent_Identity=35.5450236966825, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24657054, Length=210, Percent_Identity=36.1904761904762, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24644950, Length=214, Percent_Identity=36.4485981308411, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356425, Length=211, Percent_Identity=32.7014218009479, Blast_Score=116, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45553081, Length=212, Percent_Identity=33.0188679245283, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221378297, Length=234, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI20129731, Length=208, Percent_Identity=36.5384615384615, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24662781, Length=213, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24662789, Length=188, Percent_Identity=34.5744680851064, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24662785, Length=214, Percent_Identity=32.7102803738318, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281366140, Length=177, Percent_Identity=29.9435028248588, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.283 [H]

Molecular weight: Translated: 21429; Mature: 21429

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLIDTAASYQNEPQVGNALK
CCEEECCCCCCCCEECCHHHHHCCHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCHHHHHH
QSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAM
HCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHEEHEEEEEEEEECCCCHHHHHHHHH
EELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEVNPFNQQLHAVPWMQSRGIQP
HHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCHHHCCCCC
EAWAPFAVTGR
CCCCCEEECCC
>Mature Secondary Structure
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLIDTAASYQNEPQVGNALK
CCEEECCCCCCCCEECCHHHHHCCHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCHHHHHH
QSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAM
HCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHEEHEEEEEEEEECCCCHHHHHHHHH
EELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEVNPFNQQLHAVPWMQSRGIQP
HHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCHHHCCCCC
EAWAPFAVTGR
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]