BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is aroH

Identifier: 209398312

GI number: 209398312

Start: 2294443

End: 2295489

Strand: Direct

Name: aroH

Synonym: ECH74115_2422

Alternate gene names: 209398312

Gene position: 2294443-2295489 (Clockwise)

Preceding gene: 209400227

Following gene: 209400588

Centisome position: 41.18

GC content: 52.72

Gene sequence:

>1047_bases
ATGAACAGAACTGACGAACTCCGTACTGCGCGTATTGAGAGCCTGGTAACGCCCGCCGAACTCGCGCTACGGTATCCCGT
AACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTCAATGGTGAAGATAAGCGACTGT
TGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAAC
CAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAAT
CTCCGATCCAGATTTAAACGGCAGCTATCGGATAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATG
AGCTGGGCGTCCCAACCGCGACTGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCG
ATTGGCGCACGTACTACCGAAAGTCAGATCCACCGTGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGG
TACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAA
ATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTAT
CATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGATTTACCTGAACATCTGGTGGTGGATTTCAGCCA
CGGTAACTGCCAGAAGCAGCACCGTCGCCAGTTAGAAGTTTGTGAGGATATTTGTCAGCAAATCCGCAATGGCTCTACGG
CGATTGCTGGAATTATGGCGGAAAGTTTCCTGCGCGAAGGAACGCAAAAAATCGTCGGCGGTCAGCCGCTCACTTACGGT
CAATCCATTACCGACCCGTGTCTGGGCTGGGAAGATACCGAACGCCTGGTCGAAAAACTAGCCTCTGCGGTAGATACCCG
CTTCTGA

Upstream 100 bases:

>100_bases
GACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCA
ACAAATTTCTGAGACTTGTA

Downstream 100 bases:

>100_bases
ATGCGTGCCCATTCCTGATGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACACATTACTGCTTGCTCTGGTCAG
CCATAATATTGATAATAAGA

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRN
QYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYG
QSITDPCLGWEDTERLVEKLASAVDTRF

Sequences:

>Translated_348_residues
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRN
QYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYG
QSITDPCLGWEDTERLVEKLASAVDTRF
>Mature_348_residues
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRN
QYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYG
QSITDPCLGWEDTERLVEKLASAVDTRF

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP)

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family

Homologues:

Organism=Escherichia coli, GI1787996, Length=348, Percent_Identity=99.4252873563218, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1786969, Length=345, Percent_Identity=57.3913043478261, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788953, Length=329, Percent_Identity=49.2401215805471, Blast_Score=335, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319726, Length=349, Percent_Identity=49.2836676217765, Blast_Score=338, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6320240, Length=353, Percent_Identity=43.0594900849858, Blast_Score=295, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROH_ECO57 (Q8X5W4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90930
- PIR:   G85778
- RefSeq:   NP_288138.1
- RefSeq:   NP_310438.1
- ProteinModelPortal:   Q8X5W4
- SMR:   Q8X5W4
- EnsemblBacteria:   EBESCT00000028265
- EnsemblBacteria:   EBESCT00000060207
- GeneID:   912286
- GeneID:   961675
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2733
- KEGG:   ecs:ECs2411
- GeneTree:   EBGT00050000008977
- HOGENOM:   HBG670536
- OMA:   CQQIRAG
- ProtClustDB:   PRK12756
- BioCyc:   ECOL83334:ECS2411-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21225
- PIRSF:   PIRSF001361
- TIGRFAMs:   TIGR00034

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.54

Molecular weight: Translated: 38720; Mature: 38720

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPC
CCCCHHHHHHHHHHHCCHHHHHEECCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEEECCC
SIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRINH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEECH
GLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSC
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
PVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
CCCCCCCCCCCCEEHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCC
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPC
CCCCHHHHHHHHHHHCCHHHHHEECCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEEECCC
SIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRINH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEECH
GLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSC
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
PVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
CCCCCCCCCCCCEEHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCC
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796