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The map label for this gene is mcbR [H]

Identifier: 209398301

GI number: 209398301

Start: 1950616

End: 1951281

Strand: Direct

Name: mcbR [H]

Synonym: ECH74115_2056

Alternate gene names: 209398301

Gene position: 1950616-1951281 (Clockwise)

Preceding gene: 209396787

Following gene: 209399084

Centisome position: 35.01

GC content: 43.84

Gene sequence:

>666_bases
ATGCCTGGAACGGAAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGG
CGCACTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAG
CATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCAGCGCAAGCATTTACAGTTCCGGAAGTGGGGAAACGT
CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCA
AGACCTGGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATG
TAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTC
AGGATGGGACCTGGTTTACATTATCTCTATGAAGCGATTAATCCAGCGGAATTACGGGAGCATATAGAAAACTATCATCT
GTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTA
TTTTATACCAGCAATACAATCGTTAA

Upstream 100 bases:

>100_bases
AACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGA
AAACACGCGAAGGAATTAAA

Downstream 100 bases:

>100_bases
AGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCGCCATGCGATATTCACGCCGACACCATAATTTCG
CCCGGGCGCAGGTTCGTAAT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKR
QLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR

Sequences:

>Translated_221_residues
MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKR
QLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
>Mature_220_residues
PGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQ
LDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVR
MGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=221, Percent_Identity=99.5475113122172, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25224; Mature: 25093

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVS
CCCCHHHHEEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQ
HHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVRMGPGLHYLYEAINPAELRE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
HIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVS
CCCHHHHEEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQ
HHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVRMGPGLHYLYEAINPAELRE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
HIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]