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The map label for this gene is prfH [H]

Identifier: 209398288

GI number: 209398288

Start: 294150

End: 294650

Strand: Direct

Name: prfH [H]

Synonym: ECH74115_0281

Alternate gene names: 209398288

Gene position: 294150-294650 (Clockwise)

Preceding gene: 209397487

Following gene: 209397752

Centisome position: 5.28

GC content: 55.29

Gene sequence:

>501_bases
GTGCTGGAAACAGAAACGGGCCGCTACTCTGACACGCTGCGTTCGGCGCTGATTTCTCTGGATGGCGACAACGCATGGGC
GTTAAGCGAAAGTTGGTGCGGCACTATTCAGTGGATTTGTCTGAGTCCGTATCGGCCTCATCATGGGCGCAAAAACTGGT
TTCTGGGCATTGGGCGTTTTACCGCTGATGAGCAGGAACAATCGGATGCAATCCGTTATGAGACGCTGCGTCCGTCGGGG
CCGGGCGGTCAACATGTCAATAAAACCGACTCGGCGGTACGCGCCACGCATCTGGCAACCGGGATTAGCGTGAAGGTTCA
GTCAGAACGCAGCCAGCATGCTAACAAACGGCTGGCGCGATTGCTGATTGCCTGGAAGCTGGAGCAGCAGCAACAGGAAA
ATAGCGCAGTGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGAACGTTCACAGGG
ATGGCTTTTATCGAAGGATAA

Upstream 100 bases:

>100_bases
GTAGTGGGGATGCCAGATTTACACCCCGGACGCGGCTACCCGATTGGCGCAGCGTTCTTCTCTGTTGGTCGTTTTTACCC
GACAAGACGTCGCGGTAACG

Downstream 100 bases:

>100_bases
TGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAG
CAGTTCAGTCAGCAGTGTCC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICLSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRPSG
PGGQHVNKTDSAVRATHLATGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTG
MAFIEG

Sequences:

>Translated_166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICLSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRPSG
PGGQHVNKTDSAVRATHLATGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTG
MAFIEG
>Mature_166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICLSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRPSG
PGGQHVNKTDSAVRATHLATGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTG
MAFIEG

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=60, Percent_Identity=53.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1787462, Length=95, Percent_Identity=45.2631578947368, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367172, Length=131, Percent_Identity=33.587786259542, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17542784, Length=121, Percent_Identity=35.5371900826446, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321295, Length=57, Percent_Identity=56.140350877193, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921226, Length=64, Percent_Identity=56.25, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 18916; Mature: 18916

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICLSPYRPHHGRKNWFLGIGRF
CCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEECCC
TADEQEQSDAIRYETLRPSGPGGQHVNKTDSAVRATHLATGISVKVQSERSQHANKRLAR
CCCCCHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
LLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICLSPYRPHHGRKNWFLGIGRF
CCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEECCC
TADEQEQSDAIRYETLRPSGPGGQHVNKTDSAVRATHLATGISVKVQSERSQHANKRLAR
CCCCCHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
LLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]