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The map label for this gene is rnz [H]

Identifier: 209398284

GI number: 209398284

Start: 3153465

End: 3154400

Strand: Direct

Name: rnz [H]

Synonym: ECH74115_3410

Alternate gene names: 209398284

Gene position: 3153465-3154400 (Clockwise)

Preceding gene: 209397509

Following gene: 209396764

Centisome position: 56.59

GC content: 50.21

Gene sequence:

>936_bases
TTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCGGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGC
AATATTGCTAAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTAC
TGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCC
GGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTATCCGTGAATTTGT
GGAGACCGCGCTGCGGATTAGCGGCTCATGGACCGATTATCCGCTGGAAATTGTCGAAATTGGCGCTGGCGAAATTCTCG
ATGATGGCCTGCGCAAAGTGACCGCTTATCCAATGGAACACCCACTGGAATGCTATGGCTATCGTATTGAAGAACATGAT
AAACCGGGTGCATTAAATGCCCAGGCATTAAAAGCTGCTGGCGTACCGCCTAGCCCACTGTTTCAGGAATTAAAAGCGGG
CAAAACAATCATGCTGGAAGATGGAAGGCAGATTAACGGCGCAGATTACTTAGCTGCTCCAGTGCCAGGTAAAGCGCTCG
CTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACG
CTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGTCATAGCTCTACACGCCAGGCTGCGACACTGGCCCGTGAGGC
TGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCA
GGTCAATTTTCCCGGCGACTGAACTAGCGAATGATTTCACCGTGTTTAACGTTTAA

Upstream 100 bases:

>100_bases
ATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTATGCCT
TATACTAGCCGGAATATTTT

Downstream 100 bases:

>100_bases
CTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACTCACACGGGCATA
TTGAGCGATTTTTGATATTT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKRDELMELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLP
GLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHD
KPGALNAQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEAT
LDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV

Sequences:

>Translated_311_residues
MKRDELMELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLP
GLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHD
KPGALNAQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEAT
LDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV
>Mature_311_residues
MKRDELMELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLP
GLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHD
KPGALNAQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEAT
LDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA [H]

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family [H]

Homologues:

Organism=Homo sapiens, GI8922122, Length=361, Percent_Identity=34.0720221606648, Blast_Score=204, Evalue=6e-53,
Organism=Homo sapiens, GI260166619, Length=290, Percent_Identity=27.2413793103448, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI260166622, Length=295, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI145553959, Length=295, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI87082078, Length=305, Percent_Identity=99.016393442623, Blast_Score=623, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI71985808, Length=294, Percent_Identity=28.2312925170068, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985814, Length=294, Percent_Identity=28.2312925170068, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652403, Length=290, Percent_Identity=28.2758620689655, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR013471
- InterPro:   IPR013469 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.26.11 [H]

Molecular weight: Translated: 33781; Mature: 33781

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDELMELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNP
CCHHHHHHHHEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHCCCC
GKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDY
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
PLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALNAQALKAAGVPPSPL
CEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHCCEEECCCCCCCCCHHHHHHCCCCCCHH
FQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEAT
HHHHHCCCEEEEECCCEECCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEE
LDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPAT
EEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCHH
ELANDFTVFNV
HHCCCEEEEEC
>Mature Secondary Structure
MKRDELMELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNP
CCHHHHHHHHEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHCCCC
GKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDY
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
PLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALNAQALKAAGVPPSPL
CEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHCCEEECCCCCCCCCHHHHHHCCCCCCHH
FQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEAT
HHHHHCCCEEEEECCCEECCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEE
LDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPAT
EEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCHH
ELANDFTVFNV
HHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA