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The map label for this gene is elaD

Identifier: 209396764

GI number: 209396764

Start: 3154591

End: 3155811

Strand: Direct

Name: elaD

Synonym: ECH74115_3412

Alternate gene names: 209396764

Gene position: 3154591-3155811 (Clockwise)

Preceding gene: 209398284

Following gene: 209399997

Centisome position: 56.61

GC content: 34.97

Gene sequence:

>1221_bases
ATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACCCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGAC
CGAGGAATTACTGCAGTCTTTAAAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGT
TAGGTTATGATGAATTTGGGAAAAAAGCTGAAGATATTCTTTACCATATTGTTAGAAACCCAACAAATGATACCCTATCG
ATTATCAAGCTTATAAAAAATGCCTGTTTAAAATTATATAATTTGGCACATACCGCAACCAAACACCCCCTCAAATCACA
TGATTCAGATAATCTCCTGTTCAAAAAACTATTCTCCCCTTCGAAATTAATGGCAATTATCGGTGAGGACATTCCTCTCA
TATCAGAAAAACAGTCACTTTCAAAGGTACTTTTAAATGATAAGAATAATGAACTGAGCGATGGGACAAACTTCTGGGAT
AAAAATCGTCAATTAACCACAGATGAAATAGCTTGCTATCTTAAAAAGATCGCCGCCAATGCAAAAAATACTCAAGTCAA
TTATCCTACTGATTTCTACCTCCCCAATTCCAACAGCACTTACCTGGAAGTCGCTCTCAATGATAATATTAAGAGCGATC
CATCATGGCCGAAAGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTT
AATGAAAAAAACAACACACAACAAATAAAATGCATCATATTCAATTCATTACGTGCACTAGGCCATGAAAAAGAAAATTC
ACTTAAGCGTATCATTAACAGTTTCAATAGTTTCAATTGTGACCCCACGAGAGAAACGCCGAATAATAAGAATATAACAG
ATCATTTAACTGAACCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTG
TGCATGGCAGCCCAGGAAGTGATTGAACAAATGGAAAGCAATTCTGACAGCGCCCCCTATACGTTATTAAAAAACTATGC
TGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGTCTGGAAAACAGAAATTGTTATT
TAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAAAACTCACACCCCAAAAATAGAGCA
TCAAGCAAAAGAGTAAGTTAA

Upstream 100 bases:

>100_bases
TTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTTATCTTCATTCAGCACAACCTTT
GCAACTAAAAGGAGCATATG

Downstream 100 bases:

>100_bases
CATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAAAAGTCATCACTGACTG
ATGTCAGTCACACCACCCGC

Product: deubiquitinase

Products: NA

Alternate protein names: Deubiquitinase; Deubiquitinating enzyme; DUB; Deubiquitinating protease

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLS
IIKLIKNACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFV
CMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEIKNSHPKNRA
SSKRVS

Sequences:

>Translated_406_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLS
IIKLIKNACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFV
CMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEIKNSHPKNRA
SSKRVS
>Mature_406_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLS
IIKLIKNACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFV
CMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEIKNSHPKNRA
SSKRVS

Specific function: Protease that can act as an efficient and specific deubiquitinating enzyme in vitro. Does not possess desumoylating and deneddylating activities. The physiological substrate is unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C79 family

Homologues:

Organism=Escherichia coli, GI1788604, Length=406, Percent_Identity=87.6847290640394, Blast_Score=723, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ELAD_ECO57 (Q8XCY9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91023
- PIR:   F85867
- RefSeq:   NP_288847.1
- RefSeq:   NP_311184.1
- ProteinModelPortal:   Q8XCY9
- EnsemblBacteria:   EBESCT00000028285
- EnsemblBacteria:   EBESCT00000055807
- GeneID:   916865
- GeneID:   957300
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3529
- KEGG:   ecs:ECs3157
- GeneTree:   EBGT00050000009525
- HOGENOM:   HBG467152
- OMA:   MNICVNS
- ProtClustDB:   PRK11836
- BioCyc:   ECOL83334:ECS3157-MONOMER
- GO:   GO:0006508
- InterPro:   IPR003653

Pfam domain/function: PF02902 Peptidase_C48

EC number: NA

Molecular weight: Translated: 46517; Mature: 46517

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 231-231 ACT_SITE 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEESIELLHNIALGYDEFG
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHC
KKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
SKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWDKNRQLTTDEIACYLKKIAAN
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
AKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
CCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHH
NEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPE
CCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
IIFLHADLQQYLSQSCGAFVCMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYE
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
IDFQHRLENRNCYLDKYGDANINHYYRNLEIKNSHPKNRASSKRVS
CCHHHHCCCCCEEEECCCCCCCHHHEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEESIELLHNIALGYDEFG
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHC
KKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
SKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWDKNRQLTTDEIACYLKKIAAN
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
AKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
CCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHH
NEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPE
CCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
IIFLHADLQQYLSQSCGAFVCMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYE
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
IDFQHRLENRNCYLDKYGDANINHYYRNLEIKNSHPKNRASSKRVS
CCHHHHCCCCCEEEECCCCCCCHHHEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796