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The map label for this gene is gutB [H]

Identifier: 209398191

GI number: 209398191

Start: 2365686

End: 2366729

Strand: Reverse

Name: gutB [H]

Synonym: ECH74115_2498

Alternate gene names: 209398191

Gene position: 2366729-2365686 (Counterclockwise)

Preceding gene: 209397498

Following gene: 209396071

Centisome position: 42.47

GC content: 46.55

Gene sequence:

>1044_bases
ATGAAAAATTCAAAAGCAATATTGCAGGTGCCGGGCACAATGAAAATTATTTCAGCAGAAATACCAGTGCCTAAAGAAGA
TGAAGTTTTGATTAAAGTAGAATATGTCGGTATTTGTGGTTCAGATGTACATGGTTTTGAATCAGGTCCGTTTATTCCGC
CTAAAGACCCAAATCAAGAAATTGGCCTGGGTCATGAATGCGCCGGGACGGTTGTGGCTGTGGGAAGCCGTGTGCGCAAA
TTTAAACCGGGGGATCGGGTAAATATCGAACCTGGCGTTCCTTGCGGTCACTGTCGTTACTGTCTGGAAGGCAAATATAA
CATCTGCCCGGACGTTGATTTTATGGCGACACAACCCAACTACCGCGGCGCATTAACGCACTATCTGTGTCATCCGGAGA
GCTTTACTTACAAACTGCCCGACAATATGGACACGATGGAAGGGACGCTGGTGGAGCCTGCCGCAGTCGGGATGCATGCC
GCGATGCTGGCAGATGTTAAACCGGGTAAGAAGATAATTATTCTGGGAGCAGGTTGTATTGGTTTGATGACGTTGCAAGC
GTGCAAATGCCTGGGAGCAACGGAAATTGCCGTCGTTGATGTGCTGGAAAAACGTCTGGCAATGGCGGAACAGCTTGGTG
CGACAGTGGTTATTAACGGCGCAAAAGAAGACACTATTGCACGCTGTCAGCAATTTACCGAAGACATGGGCGCAGATATT
GTTTTCGAAACAGCGGGTTCTGCGGTCACCGTTAAACAGGCACCTTATCTGGTAATGCGTGGCGGTAAAATTATGATTGT
TGGTACTGTACCCGGCGCTTCGGCAATCAATTTCCTCAAAATCAATCGCGAAGTCACTATCCAGACGGTATTCCGCTATG
CCAATCGTTATCCGGTCACGATTGAAGCAATTTCTTCAGGGCGATTCGATGTGAAATCGATGGTGACGCATATTTACGAT
TATCGGGATGTACAACAGGCATTTGAAGAGTCAGTTAACAACAAACGCGACATTATTAAAGGCGTTATTAAGATTAGCGA
TTAA

Upstream 100 bases:

>100_bases
GCAATTGCTATCGCCACCATTGGAATCGAAACCAAAGGTGTCTCCGTTGAAAGTTTAAGTATTGATGCAGTAGCCAATAA
ATAATACGGGTGGATTCAAA

Downstream 100 bases:

>100_bases
TTAATACGGGAGAACAGAACATGCTCGCAGATATCAGATATTGGGAAAACGATGCCACAAATAAACGTTATGCTATTGCC
CATTTTAATGTATGGAATGC

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD

Sequences:

>Translated_347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD
>Mature_347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=333, Percent_Identity=38.4384384384384, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI4501929, Length=355, Percent_Identity=27.0422535211268, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI34577061, Length=354, Percent_Identity=26.271186440678, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4501933, Length=358, Percent_Identity=27.0949720670391, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI156523966, Length=361, Percent_Identity=27.7008310249307, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=27.7008310249307, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI71743840, Length=340, Percent_Identity=26.1764705882353, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI262073058, Length=340, Percent_Identity=26.1764705882353, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=25.8855585831063, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788073, Length=347, Percent_Identity=99.4236311239193, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1790045, Length=329, Percent_Identity=28.8753799392097, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1788407, Length=335, Percent_Identity=28.955223880597, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1787863, Length=345, Percent_Identity=26.9565217391304, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790718, Length=308, Percent_Identity=29.2207792207792, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788075, Length=339, Percent_Identity=27.7286135693215, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI226510992, Length=343, Percent_Identity=28.2798833819242, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI87082125, Length=268, Percent_Identity=30.9701492537313, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=27.7929155313352, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081918, Length=259, Percent_Identity=30.1158301158301, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562876, Length=351, Percent_Identity=36.4672364672365, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17562878, Length=344, Percent_Identity=36.6279069767442, Blast_Score=201, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71988145, Length=260, Percent_Identity=30.3846153846154, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25146526, Length=368, Percent_Identity=23.3695652173913, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=265, Percent_Identity=29.4339622641509, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=370, Percent_Identity=23.2432432432432, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562582, Length=274, Percent_Identity=25.5474452554745, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=350, Percent_Identity=36.2857142857143, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6322619, Length=352, Percent_Identity=34.0909090909091, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319955, Length=352, Percent_Identity=34.0909090909091, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319257, Length=353, Percent_Identity=30.028328611898, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319258, Length=361, Percent_Identity=27.4238227146814, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320033, Length=366, Percent_Identity=27.3224043715847, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324486, Length=289, Percent_Identity=26.9896193771626, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=272, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=334, Percent_Identity=27.5449101796407, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=308, Percent_Identity=26.2987012987013, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=39.4886363636364, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17737897, Length=353, Percent_Identity=38.2436260623229, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45551930, Length=327, Percent_Identity=25.3822629969419, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550770, Length=327, Percent_Identity=25.3822629969419, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221457811, Length=327, Percent_Identity=25.3822629969419, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 37687; Mature: 37687

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00059 ADH_ZINC ; PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQE
CCCCCEEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
IGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPN
CCCCCHHCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCCEEECCCC
YRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHAAMLADVKPGKKIIILGAGCI
CCCCCEEEEECCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHEECCCCCEEEEEECCHH
GLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMGADI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCE
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAINFLKINREVTIQTVFRYANRYPVT
EEECCCCEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHCCCCEE
IEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKISD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
>Mature Secondary Structure
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQE
CCCCCEEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
IGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPN
CCCCCHHCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCCEEECCCC
YRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHAAMLADVKPGKKIIILGAGCI
CCCCCEEEEECCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHEECCCCCEEEEEECCHH
GLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMGADI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCE
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAINFLKINREVTIQTVFRYANRYPVT
EEECCCCEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHCCCCEE
IEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKISD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]