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The map label for this gene is dnaB

Identifier: 209398031

GI number: 209398031

Start: 5192669

End: 5194084

Strand: Direct

Name: dnaB

Synonym: ECH74115_5554

Alternate gene names: 209398031

Gene position: 5192669-5194084 (Clockwise)

Preceding gene: 209395779

Following gene: 209397944

Centisome position: 93.19

GC content: 53.39

Gene sequence:

>1416_bases
ATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCC
TCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTGGATAACGAACGCTGGGATGATGTAGCCGAGC
GTGTGGTAGCAGACGATTTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGT
CCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGC
AGAGTTGTCAAAAAATACGCCAAGTGCTGCGAACATCAGTTCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTG
AGATGATCTCGGTTGCGAATGAGATTGCCGAAGCTGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGATCTG
GCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCGAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGA
CGCAACCGTGGCGCGTATTGAGCAGTTATTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGATGATC
TCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTT
GCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATTTTCTCGCTGGAGATGCCATCAGAACA
GATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACT
GGGCGCGCATTTCCGGCACCATGGGTATTTTGCTCGAAAAACGCAATATCTATATCGATGACTCCTCCGGCCTGACGCCA
ACGGAAGTGCGTTCCCGCGCACGCCGTATTGCCCGTGAACACGGCGGCATCGGGCTTATCATGATCGACTACCTGCAACT
GATGCGCGTACCGGCACTTTCCGATAACCGTACGCTGGAAATTGCAGAAATCTCCCGCTCGCTGAAAGCACTGGCGAAAG
AACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGTCCGGTCAACTCC
GACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAA
CAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTA
ACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAA

Upstream 100 bases:

>100_bases
TTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGGTAAAGTTTCGACCCATTCTTTA
TCTCGGTAACTCCATTCACT

Downstream 100 bases:

>100_bases
TAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACC
GCCGCGCTCTGCGACACAAC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE

Sequences:

>Translated_471_residues
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
>Mature_470_residues
AGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSP
IDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLA
ESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFA
MNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPT
EVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSD
LRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=99.7876857749469, Blast_Score=958, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_ECO57 (Q8X5V3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91258
- PIR:   F86098
- RefSeq:   NP_290685.1
- RefSeq:   NP_313061.1
- ProteinModelPortal:   Q8X5V3
- SMR:   Q8X5V3
- EnsemblBacteria:   EBESCT00000028430
- EnsemblBacteria:   EBESCT00000056432
- GeneID:   914301
- GeneID:   960072
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5650
- KEGG:   ecs:ECs5034
- GeneTree:   EBGT00050000008962
- HOGENOM:   HBG734566
- OMA:   TGYTDLD
- ProtClustDB:   PRK08006
- BioCyc:   ECOL83334:ECS5034-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- Gene3D:   G3DSA:1.10.860.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00665

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C; SSF48024 DNA_helicase_DnaB-like_N

EC number: =3.6.4.12

Molecular weight: Translated: 52407; Mature: 52276

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51199 SF4_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCHHCCCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHH
SYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEH
IAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCHHCCCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHH
SYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEH
IAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796