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The map label for this gene is alr

Identifier: 209397944

GI number: 209397944

Start: 5194137

End: 5195216

Strand: Direct

Name: alr

Synonym: ECH74115_5555

Alternate gene names: 209397944

Gene position: 5194137-5195216 (Clockwise)

Preceding gene: 209398031

Following gene: 209395724

Centisome position: 93.22

GC content: 55.56

Gene sequence:

>1080_bases
ATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCACCTGCCAG
TAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACG
CCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGC
TTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGC
GCTGGAAGAGGCTAGCCTGGACGAGCCGGTTACCGTCTGGATGAAACTCGATACCGGTATGCACCGTCTGGGCGTAAGGC
CGGAACAGGCTGAGGCGTTTTATCATCGCCTGACCCAGTGCAAAAACGTTCGGCAGCCGGTGAATATCGTCAGCCATTTT
GCGCGCGCGGATGAACCAAAATGTGGCGCAACCGAGAAACAACTCGCTATCTTTAATACCTTTTGCGAAGGCAAACCAGG
TCAACGTTCCATTGCCGCGTCGGGTGGCATTCTGCTGTGGCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTC
TTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGC
CTGATTGCCGTGCGTGAGCATAAAGTCGGAGAGCCTGTCGGTTATGGTGGAACCTGGATAAGCGAACGTGATACTCGTCT
TGGTGTAGTTGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTACGCCAGTGCTGGTGAACGGTCGCG
AAGTGCCGATTGTCGGGCGCGTGGCGATGGATATGATCTGCGTAGACTTAGGTCCACAGGCACAGGATAAAGCCGGGGAC
CCGGTCATTTTGTGGGGTGAAGGTTTGCCCGTAGAACGTATCGCTGAAATGACGAAAGTAAGCGCTTACGAACTTATTAC
GCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAA

Upstream 100 bases:

>100_bases
TGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAA
ACACTTATCAAGGAACACAA

Downstream 100 bases:

>100_bases
TCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCCTACCAATTCAATA
AGTTGATATTCTTTCATGCT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD

Sequences:

>Translated_359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
>Mature_359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD

Specific function: Provides the D-alanine required for cell wall biosynthesis

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1790487, Length=359, Percent_Identity=99.4428969359331, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1787439, Length=346, Percent_Identity=41.907514450867, Blast_Score=275, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR1_ECO57 (Q8X5V2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91258
- PIR:   G86098
- RefSeq:   NP_290686.1
- RefSeq:   NP_313062.1
- ProteinModelPortal:   Q8X5V2
- SMR:   Q8X5V2
- EnsemblBacteria:   EBESCT00000025766
- EnsemblBacteria:   EBESCT00000055339
- GeneID:   914300
- GeneID:   960071
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5651
- KEGG:   ecs:ECs5035
- GeneTree:   EBGT00050000009862
- HOGENOM:   HBG712172
- OMA:   EGTPVWV
- ProtClustDB:   PRK00053
- BioCyc:   ECOL83334:ECS5035-MONOMER
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 39196; Mature: 39196

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 34-34 ACT_SITE 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHH
EEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVW
HHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
MKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT
EEECCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
HHCCCCCCCEEECCCCEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCEEHH
VAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHH
EEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVW
HHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
MKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT
EEECCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
HHCCCCCCCEEECCCCEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCEEHH
VAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796