BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is cbiO1 [H]

Identifier: 209398012

GI number: 209398012

Start: 3918335

End: 3919012

Strand: Direct

Name: cbiO1 [H]

Synonym: ECH74115_4230

Alternate gene names: 209398012

Gene position: 3918335-3919012 (Clockwise)

Preceding gene: 209399401

Following gene: 209400876

Centisome position: 70.32

GC content: 53.24

Gene sequence:

>678_bases
ATGTTAACGTTAAATCAGATATCTTATCGCTGGCCTGGCGCGGCAACGGATTGTCTTTGCGACATTTCTCTGCAACTCAA
ACAAGGTGAATGGTTGGCGTTGACCGGCGACAATGGCGCAGGGAAATCGACGCTGTTACGAGTAATGGCAGGTCTTCTTA
CGCCCACAGCCGGTACGGTTATGCTGCAACAACAGGCGATGAAAAACCTGAAAAACCGCCAGCGCGCGGCAAAGATTGGC
GTACTGTTTCAGGAAGCGGAAAACCAACTTTTTCACAGCACCGTCGCTGACGAGATCGCATTTGGTTTGAAACTGCAAAA
ATGCCCGGCGGATGAAATCACTCAACGTACCCATGCTGCCCTGCAATGTTGTCAGTTAGCAGACACTGCCAGCGCGCACC
CGTTGGATCTGCATAGTGCGCAGCGACGGATGGTCGCCGTCGCCTGCCTGGAAGCACTCTCTCCGCCACTATTGCTGCTG
GACGAGCCGAGTCGGGATTTTGACGAAAATTGGTTGAGCGTGTTTGAAAGCTGGCTGGAGAAGTGCCGCCAGCGAGGCAC
GAGCGTCGTAGCAATCAGTCATGACGCCGCATTTACCCGGCGTCATTTCTCTCGTGTGGTGCGACTGGAAGATGGTCTAA
TCAGGAACATTAATCCACCAGACGATATTCACCCGTAG

Upstream 100 bases:

>100_bases
AACGTGGTTCGCTGATTATTCTTTTTGAACGTCATCGCACACCTTTCCTGAACTACTGCCAGCAAGCGTGGCAATTGCAA
AACGGAGCGCTCCAGCCACT

Downstream 100 bases:

>100_bases
CGGTCATCATTAAGGTCAAATTTGCAGGCAGACTCTCTTCCAGCACCCGGCGAACATTCGGCCCGTCGGTACGGTCATAA
AAAGCTTCTGCGTCTGCCAG

Product: ABC transporter, ATP-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIG
VLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNINPPDDIHP

Sequences:

>Translated_225_residues
MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIG
VLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNINPPDDIHP
>Mature_225_residues
MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIG
VLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNINPPDDIHP

Specific function: Part of the ABC transporter complex CbiONQ involved in cobalt import. Responsible for energy coupling to the transport system [H]

COG id: COG1118

COG function: function code P; ABC-type sulfate/molybdate transport systems, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI4505771, Length=232, Percent_Identity=28.0172413793103, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI9961252, Length=232, Percent_Identity=28.0172413793103, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI9961250, Length=239, Percent_Identity=27.1966527196653, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI42741659, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI255708477, Length=224, Percent_Identity=25.8928571428571, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI9955963, Length=187, Percent_Identity=29.9465240641711, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI148612844, Length=206, Percent_Identity=25.7281553398058, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787370, Length=212, Percent_Identity=33.4905660377358, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87081834, Length=214, Percent_Identity=32.7102803738318, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788761, Length=215, Percent_Identity=35.3488372093023, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789873, Length=215, Percent_Identity=30.2325581395349, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87081791, Length=206, Percent_Identity=34.9514563106796, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1790467, Length=206, Percent_Identity=29.6116504854369, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789032, Length=208, Percent_Identity=29.3269230769231, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786398, Length=207, Percent_Identity=29.4685990338164, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082267, Length=225, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788225, Length=206, Percent_Identity=29.126213592233, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787029, Length=218, Percent_Identity=27.0642201834862, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790739, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787089, Length=208, Percent_Identity=30.2884615384615, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87081709, Length=226, Percent_Identity=26.5486725663717, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787712, Length=205, Percent_Identity=27.3170731707317, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786253, Length=199, Percent_Identity=32.1608040201005, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1790525, Length=202, Percent_Identity=28.7128712871287, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI48995001, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786563, Length=192, Percent_Identity=32.2916666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787164, Length=214, Percent_Identity=31.3084112149533, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789672, Length=230, Percent_Identity=25.6521739130435, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786803, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788897, Length=209, Percent_Identity=29.1866028708134, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789891, Length=207, Percent_Identity=30.9178743961353, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=26.7543859649123, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788472, Length=216, Percent_Identity=24.0740740740741, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787105, Length=226, Percent_Identity=26.9911504424779, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787792, Length=202, Percent_Identity=30.1980198019802, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788210, Length=201, Percent_Identity=29.8507462686567, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788506, Length=212, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787759, Length=217, Percent_Identity=27.1889400921659, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790190, Length=206, Percent_Identity=25.2427184466019, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789864, Length=230, Percent_Identity=25.2173913043478, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786345, Length=231, Percent_Identity=27.7056277056277, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786698, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786703, Length=201, Percent_Identity=27.8606965174129, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367384, Length=194, Percent_Identity=27.319587628866, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787500, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787547, Length=202, Percent_Identity=23.2673267326733, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786319, Length=194, Percent_Identity=26.8041237113402, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081782, Length=223, Percent_Identity=29.5964125560538, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788450, Length=223, Percent_Identity=25.1121076233184, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1788644, Length=235, Percent_Identity=22.9787234042553, Blast_Score=60, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=229, Percent_Identity=25.764192139738, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17558664, Length=228, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17569143, Length=228, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=223, Percent_Identity=23.3183856502242, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=234, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=234, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=234, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI212646699, Length=207, Percent_Identity=25.1207729468599, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=161, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=229, Percent_Identity=23.5807860262009, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=197, Percent_Identity=24.3654822335025, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666092, Length=203, Percent_Identity=26.6009852216749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573573, Length=176, Percent_Identity=28.4090909090909, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28573571, Length=176, Percent_Identity=28.4090909090909, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR015856
- InterPro:   IPR003593
- InterPro:   IPR022112 [H]

Pfam domain/function: PF00005 ABC_tran; PF12370 CoABC_C [H]

EC number: NA

Molecular weight: Translated: 24968; Mature: 24968

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGTV
CCCCCCCCCCCCCCCCHHEEECEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHH
MLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRTHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHH
LQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLLDEPSRDFDENWLSVFESWLE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
KCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNINPPDDIHP
HHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGTV
CCCCCCCCCCCCCCCCHHEEECEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHH
MLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRTHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHH
LQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLLDEPSRDFDENWLSVFESWLE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
KCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNINPPDDIHP
HHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]