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The map label for this gene is pgaA

Identifier: 209397134

GI number: 209397134

Start: 1275095

End: 1277518

Strand: Reverse

Name: pgaA

Synonym: ECH74115_1266

Alternate gene names: 209397134

Gene position: 1277518-1275095 (Counterclockwise)

Preceding gene: 209400320

Following gene: 209397733

Centisome position: 22.93

GC content: 47.32

Gene sequence:

>2424_bases
ATGTATTCAAGTAGCAGAAAAAGGTGCCCGAAAACCAAATGGGCTTTGAAACTTCTTACTGCCGCATTTTTAGCAGCGAG
TCCCGCGGCGAAGAGTGCTGTTAATAACGCCTATGATGCATTGATTATTGAAGCTCGCAAGGGTAATACTCAGCCAGCTT
TGTCATGGTTTGCACTAAAATCAGCACTCAGCAATAACCAAATTGCTGACTGGTTACAGATTGCCTTATGGGCCGGGCAA
GATAAACAGGTTATTACCGTTTACAACCGCTACCGTCATCAGCAATTACCAGCGCGTGGTTATGCAGCTGTCGCCGTCGC
TTATCGTAACCTGCAACAATGGCAAAACTCGCTTACACTGTGGCAAAAGGCGCTCTCTCTGGAGCCGCAAAATAAGGATT
ATCAACGGGGACAAATTTTAACCCTGGCAGATGCTGGTCACTATGATACTGCGCTGGTTAAACTTAAGCAGCTTAACTCT
GGAGCACCGGACAAAGCCAATTTACTCGCAGAAGCCTATATCTATAAACTGGCGGGGCGTCATCAGGATGAATTACGGGC
GATGACAGAGTCATTACCTGAAAATGCATCTACGCAACAATATCCCACAGAATACGTGCAGGCATTACGTAATAATCAAC
TTGCTGCCGCGATTGACGATGCCAATTTAACGCCAGATATTCGCGCTGATATTCATGCCGAACTGGTCAGACTGTCGTTT
ATGCCTACGCGCAGTGAAAGTGAACGTTATGCCATTGCCGATCGCGCCCTCGCCCAATACGCTGCATTAGAAATTCTGTG
GCACGATAACCCAGACCGTACTGCCCAGTACCAGCGTATTCAGGTTGATCATCTTGGCGCGTTATTAACTCGCGATCGTT
ATAAAGACGTTATTTCTCACTATCAGCGATTAAAAAAGACGGGGCAAATTATTCCGCCCTGGGGGCAATATTGGGTTGCA
TCGGCTTATCTCAAAGATCATCAGCCGAAAAAAGCACAGTCAATAATGACCGAGCTCTTTTATCACAAGGAGACCATTGC
CCCGGATTTATCCGATGAAGAACTTGCGGATCTCTTTTACAGCCACCTGGAGAGTGAAAATTATCCGGGCGCGCTAACTG
TCACCCAACATACCATTAATACTTCGCCGCCTTTCCTTCGGTTAATGGGCACGCCTACGAGCATCCCGAATGATACCTGG
TTACAGGGGCATTCGTTTCTCTCAACCGTAGCAAAATATAGTAATGATCTTCCTCAGGCTGAAATGACAGCCAGAGAGCT
TGCTTATAACGCACCAGGAAATCAGGGACTGCGCATTGATTACGCGAGTGTGTTACAAGCCCGCGGTTGGCCTCGTGCAG
CAGAAAATGAATTAAAAAAAGCAGAAGTGATCGAGCCACGTAATATTAATCTGGAGGTTGAACAAGCCTGGACAGCATTA
ACGTTACAAGAATGGCAGCAGGCAGCTGTCTTAACGCACGATGTTGTCGAACGTGAACCGCAAGATCCCGGCGTTGTACG
ATTAAAACGTGCGGTTGATGTACATAATCTTGCAGAGCTTCGTATCGCTGGCTCAACAGGAATTGATGCCGAAGGCCCGG
ATAGTGGTAAACATGATGTCGACTTAACCACCATCGTTTATTCACCACCGCTGAAGGATAACTGGCGCGGTTTTGCTGGA
TTCGGTTATGCCGATGGACAATTTAGCGAAGGAAAAGGGATTGTTCGCGACTGGCTTGCGGGTGTTGAGTGGCGGTCACG
TAATATCTGGCTCGAGGCAGAGTACGCTGAACGCGTTTTCAATCATGAGCATAAACCCGGCGCGCGCCTGTCTGGCTGGT
ATGATTTTAATGATAACTGGCGTATTGGTTCGCAACTGGAACGCCTCTCTCACCGCGTTCCATTACGGGCAATGAAAAAT
GGTGTTACAGGCAACAGTGCTCAGGCTTATGTTCGCTGGTATCAAAATGAGCGGCGTAAGTACGGTGTCTCCTGGGCTTT
CACTGATTTTTCCGACAGTAACCAGCGTCATGAAGTCTCACTTGAGGGTCAGGAACGCATCTGGTCTTCACCATATTTGA
TTGTCGATTTCCTACCCAGTCTGTATTACGAACAAAATACAGAACACGATACCCCATACTACAACCCTATAAAAACGTTC
GATATTGTTCCGGCATTTGAGGCAAGCCATTTGTTATGGCGAAGCTATGAAAATAGCTGGGAGCAAATATTCAGCGCAGG
TGTTGGTGCCTCCTGGCAAAAACATTATGGCACGGATGTCGTCACCCAACTCGGCTACGGGCAACGCATTAGCTGGAATG
ACGTGATTGATGCTGGCGCAACGCTACGCTGGGAAAAACGACCTTATGACGGTGACAGAGAACACAACTTATACGTTGAA
TTCGATATGACATTCAGATTTTAA

Upstream 100 bases:

>100_bases
GCGTTTAATAACGGATTATGAGGTGCAAAAATATCTTTCTTTTCAGTTACCTGTAATTAGATACAGAGAGAGATTTTGGC
AATACATGGAGTAATACAGG

Downstream 100 bases:

>100_bases
GGATAAATATGTTACGTAATGGAAATAAATATCTCCTGATGCTGGTGAGTATAATTATGCTCACCGCGTGCATTAGCCAG
TCAAGAACATCATTTATACC

Product: outer membrane protein PgaA

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF

Sequences:

>Translated_807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF
>Mature_807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats

Homologues:

Organism=Escherichia coli, GI1787261, Length=807, Percent_Identity=100, Blast_Score=1665, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGAA_ECO57 (P69435)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F90787
- RefSeq:   NP_287031.1
- RefSeq:   NP_309297.1
- ProteinModelPortal:   P69435
- EnsemblBacteria:   EBESCT00000025152
- EnsemblBacteria:   EBESCT00000059956
- GeneID:   912850
- GeneID:   959162
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1526
- KEGG:   ecs:ECs1270
- GeneTree:   EBGT00050000011311
- HOGENOM:   HBG466902
- OMA:   LWRHYER
- ProtClustDB:   PRK10049
- BioCyc:   ECOL83334:ECS1270-MONOMER
- GO:   GO:0005488
- GO:   GO:0006810
- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734
- Gene3D:   G3DSA:1.25.40.10

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 92208; Mature: 92208

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50005 TPR; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
SALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTL
HHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIYKLAGR
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH
CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH
YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA
HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHEEEEEECCCCCCCCCCCCCCCCE
DLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVF
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCEEEEEEHHHHHHH
NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC
TLRWEKRPYDGDREHNLYVEFDMTFRF
EEEECCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
SALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTL
HHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIYKLAGR
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH
CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH
YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA
HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHEEEEEECCCCCCCCCCCCCCCCE
DLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVF
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCEEEEEEHHHHHHH
NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC
TLRWEKRPYDGDREHNLYVEFDMTFRF
EEEECCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796