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The map label for this gene is hipO [H]

Identifier: 209397697

GI number: 209397697

Start: 5070870

End: 5072036

Strand: Direct

Name: hipO [H]

Synonym: ECH74115_5424

Alternate gene names: 209397697

Gene position: 5070870-5072036 (Clockwise)

Preceding gene: 209400897

Following gene: 209396272

Centisome position: 91.01

GC content: 53.47

Gene sequence:

>1167_bases
ATGATCGAACACTATATTCGAGGGTTTGAGGAAGAACTGCGTGAGATCCGCCATCAGATCCACGAAAACCCGGAGCTGGG
ATTACAAGAATTTAAAACCAGTGCTTTGGTTGCGGAAAAGCTGCGTCAGTGGGGCTATGAAGTAGAGCAAGGACTGGCGA
CGACGGGGATAGTGGCCACGTTAAAAGTGGGCGACGGAGAGAAAAGTATTGGCCTGCGGGCGGATATGGATGCGCTGCCG
ATTTATGAAAATAGTGGCAAGCCCTGGGCCAGTAAACATCCTGGTTTAATGCACGCCTGCGGGCATGACGGGCATACCAC
CATACTGCTGGGGGCCGCGCGTTATTTTGCCGAAACCCGCCGTTTTAACGGCACGTTACGTCTTATCTTCCAGCCTGCGG
AAGAGATGATTAATGGCGGTGAGATCATGGTTAAAGAGGGGCTTTTTGATCATTTCCCCTGCGATGTCATTTTCGGCATG
CATAACATGCCCGGTCTGCCGGTGGGTAAGTTTTATTTCCAGCCAGGGGCACTGATGGCGTCAATGGATCAGTTCCATAT
TACAGTTCGTGGTTGTGGCGGACACGGTGCGATCCCGCACAAAGCCATCGATCCGGTGCTAGTTGCCGCACATATCACCA
CCGCATTACAAAGCATTGTGTCACGCAATGTCGATCCGCTGGAAGCGGCGGTAATTACCGTCGGCAGCATTGTTGCGGGT
GAGGCGGCTAACGTCATTCCTGACAGTGCTGAGATGAAAATCAGCGTCCGCTCTCTTAGCCGCGATACCCGACAACTTCT
TCTGACCCGTATTCCTGCTCTGGCACAAGCCCAGGCTGCCAGCTTTGGCGCTACGGCTGAGGTTACGCATGTTAACGGTA
CACCAGTATTAGTAAATGACGAGGAGATGGCGCGCTTCGCCTGGCAGGTGGCGTGTAAAACGTTTGGTGAAGATCGGGCC
GAGTTTGGCATCAAGCCCCTGATGGGCAGTGAAGATTTCTCTTTCATGCTGGAAGCCCAACCCAAAGGCGGTTTCCTGTT
ATTCGGTAATGGCGATGTCGGGGAAGGTTCCTGCATGGTGCATAACCCGGGTTACGACTTCAACGATGCAAGTCTGGTCC
CGGCAAGCAGCTACTGGGGCGCACTGGTAGAAGCCTGGCTGCAATAA

Upstream 100 bases:

>100_bases
AACAAACATATAAATATTTTATATTGATGAGTTATTTGTATTTACACCATCAATAAAATCCGTTAATTAGCCTGTTTATA
AGTGAATAAAGGAATAGCCG

Downstream 100 bases:

>100_bases
ATAAAACAACACACAACAACACTTTTTGGCAAGGAACATGGAATGACGGCGATCGTAAATGAAACCGTGAGTGTGAAGAA
AAAGCCCAGCGGCAGGCGGG

Product: hippuricase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALP
IYENSGKPWASKHPGLMHACGHDGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAVITVGSIVAG
EAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRA
EFGIKPLMGSEDFSFMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ

Sequences:

>Translated_388_residues
MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALP
IYENSGKPWASKHPGLMHACGHDGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAVITVGSIVAG
EAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRA
EFGIKPLMGSEDFSFMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ
>Mature_388_residues
MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALP
IYENSGKPWASKHPGLMHACGHDGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAVITVGSIVAG
EAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRA
EFGIKPLMGSEDFSFMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=426, Percent_Identity=28.4037558685446, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42190; Mature: 42190

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGIVAT
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
LKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGHDGHTTILLGAARYFAETR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHH
RFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGMHNMPGLPVGKFYFQPGALMA
HCCCEEEEEEECHHHHCCCCEEEEECCCCCCCCHHHEECCCCCCCCCCCCEEECCCHHHC
SMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAVITVGSIVAG
CCCEEEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVTHVNGTPVLVND
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCEEEECC
EEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFSFMLEAQPKGGFLLFGNGDVGEGSCMV
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEE
HNPGYDFNDASLVPASSYWGALVEAWLQ
ECCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGIVAT
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
LKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGHDGHTTILLGAARYFAETR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHH
RFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGMHNMPGLPVGKFYFQPGALMA
HCCCEEEEEEECHHHHCCCCEEEEECCCCCCCCHHHEECCCCCCCCCCCCEEECCCHHHC
SMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAVITVGSIVAG
CCCEEEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVTHVNGTPVLVND
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCEEEECC
EEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFSFMLEAQPKGGFLLFGNGDVGEGSCMV
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEE
HNPGYDFNDASLVPASSYWGALVEAWLQ
ECCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]