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The map label for this gene is citH [H]

Identifier: 209396272

GI number: 209396272

Start: 5072079

End: 5073395

Strand: Direct

Name: citH [H]

Synonym: ECH74115_5425

Alternate gene names: 209396272

Gene position: 5072079-5073395 (Clockwise)

Preceding gene: 209397697

Following gene: 209396287

Centisome position: 91.03

GC content: 51.48

Gene sequence:

>1317_bases
ATGACGGCGATCGTAAATGAAACCGTGAGTGTGAAGAAAAAGCCCAGCGGCAGGCGGGTGATTTTTGCCTCGGCGTTTGG
CAATGCGCTCGAATTTTTTGATTTTGGCGTTTACAACTTTTTTGTGGTCTATATCAGCACGCTATTTTTTCCGCCCAGTG
CAGATAAAAACGTCGCGCTACTGCTGGCGTTTGCCACTTTCGGCGTGAGCTTTTTTATGCGCCCGCTCGGCGGCATTATT
GTGGGGGCCTGGGCCGATCGTTTCGGACGCAAACCCGCGATGGTGTTCACCATTGCGCTGATGAGTCTTGGTACACTGAT
GATCGGCATCGCACCGACGTATGAAACGGCGGGCTATTGGGGAACCGCGACGCTGGTGCTAGCGCGTCTAATTCAGGGCG
TTGCGGCAGGAGGCGAAGTCGGTGCCTCAATGTCTTTGTTGGTTGAATCAGCTCCGGCAAATCGTCGCGGTTTTTACAGC
AGTTGGTCACTGGCGACTCAAGGCCTGGCAACAACTTTTGGAGGTGTTGTCGCTTTAGGTTTAAGTGCATGGCTGCCCTT
TGCGACCGGTTCAGAAACCGTCATGGCTGAATGGGGCTGGCGTGTGCCGTTTTTTATTGGCGTGTTATTAGCGCCTGTCG
GCTGTTGGTTGCGTTTGAGTCTGGAAAATGATGTTCCTGAGCCAGCACATAATAAGAAGGCCGCAGCCAGCGAAAGCGCC
TTCTCCTTACTTATGCAACATAAAGCGACTATCGCTAACGGTATCTTGCTCGCTATTGGTAGTACAGTCGCAACCTATAT
CTCCCTCTTTTATTACGGTACATGGGCGGCGAAGTATTTAGGAATGAACCAAAACTATTCTCATGCAGCGATGTTACTGG
CGGGCGTTATTACGTTTGTGGGTGCGCTGCTGGTGGGGATGTTGTGCGATTCGGTCGGGCGTAAAAAGCTGATTTTAATC
TCCCGTGTGATGGTGCTGATCTGTAGCTGGCCGTCATTCTGGCTGTTGGTGAATTACCCAAGCCCAGGCATGTTGCTGAC
GGTGGTTTTCGTGATGGTCAGCTTTACCACGCTTGGCGGTGTGCCAGTGATGTTGTTGATCTCTGAACTGTTGCCGAAAC
GGATTCGGGCATTGGGTTTTGCGCTGGTTTATAGCATTGGTGTGGCAATCTTTGGCGGTTTTGCTCAATATTTCGCTACG
CAATCCATCGTGTTGCTGGATAGTTTGACGGCTCCGGCGTGGTATCTGGGTGGAGGCACGTTGCTGTCGATGTTGGCGTT
GTTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAA

Upstream 100 bases:

>100_bases
AAGTCTGGTCCCGGCAAGCAGCTACTGGGGCGCACTGGTAGAAGCCTGGCTGCAATAAATAAAACAACACACAACAACAC
TTTTTGGCAAGGAACATGGA

Downstream 100 bases:

>100_bases
ATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTA
CTTTTGGCAAGGATTCAGAC

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGII
VGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESA
FSLLMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILI
SRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFAT
QSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ

Sequences:

>Translated_438_residues
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGII
VGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESA
FSLLMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILI
SRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFAT
QSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ
>Mature_437_residues
TAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIV
GAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSS
WSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESAF
SLLMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILIS
RVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQ
SIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=33.2574031890661, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1790550, Length=447, Percent_Identity=29.9776286353468, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=27.9342723004695, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=26.9430051813472, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082231, Length=329, Percent_Identity=27.6595744680851, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46993; Mature: 46862

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVAL
CCCEECCCHHCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALG
HHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESA
HHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
FSLLMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GALLVGMLCDSVGRKKLILISRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
VPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCHH
LLSMLALLYVKEPAKELQ
HHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
TAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVAL
CCEECCCHHCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALG
HHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESA
HHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
FSLLMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GALLVGMLCDSVGRKKLILISRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
VPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCHH
LLSMLALLYVKEPAKELQ
HHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]