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The map label for this gene is pntA [H]

Identifier: 209397404

GI number: 209397404

Start: 2182467

End: 2184053

Strand: Reverse

Name: pntA [H]

Synonym: ECH74115_2313

Alternate gene names: 209397404

Gene position: 2184053-2182467 (Counterclockwise)

Preceding gene: 209399051

Following gene: 209396936

Centisome position: 39.2

GC content: 52.43

Gene sequence:

>1587_bases
GTGGCTCCTGACACAGGCAAACCATCATCAATAAAACCGATGGAAGGGAATATCATGCGAATTGGCATACCAAGAGAACG
GTTAACCAATGAACCCCGTGTTGCAGCAACGCCAAAAACAGTGGAACAGCTGCTGAAACTGGGGTTTACCGTCGCGGTAG
AGAGCGGCGCGGGTCAACTGGCAAGTTTTGACGATAAAGCGTTTGTGCAAGCGGGCGCTGAAATTGTAGAAGGGAATAGC
GTCTGGCAGTCAGAGATCATTCTGAAGGTCAATGCGCCGTTAGATGATGAAATTGCGTTACTGAATCCTGGGACAACGCT
GGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAATTAATGCAAAAACTTGCGGAACGTAACGTGACCGTGATGGCGATGG
ACTCTGTGCCGCGTATCTCACGCGCACAATCGCTGGACGCACTAAGCTCGATGGCGAATATCGCCGGTTATCGCGCCATT
GTTGAAGCGGCACATGAATTTGGGTGCTTCTTTACCGGGCAAATTACTGCGGCCGGGAAAGTGCCACCGGCAAAAGTGAT
GGTGATTGGTGCGGGTGTTGCAGGTCTGGCCGCCATTGGCGCAGCAAACAGTCTCGGCGCGATTGTGCGTGCATTCGACA
CCCGCCCGGAAGTGAAAGAACAAGTTCAAAGTATGGGCGCGGAATTCCTCGAGCTGGATTTTAAAGAGGAAGCGGGCAGC
GGCGATGGCTATGCCAAAGTGATGTCGGACGCGTTCATCAAAGCGGAAATGGAACTCTTTGCCGCCCAGGCAAAAGAGGT
CGATATCATTGTCACCACCGCGCTTATTCCAGGCAAACCAGCGCCGAAGCTAATTACCCGTGAAATGGTTGACTCCATGA
AGGCGGGCAGTGTGATTGTCGACCTGGCAGCCCAAAACGGCGGCAACTGTGAATACACCGTGCCGGGTGAAATCTTCACT
ACGGAAAATGGTGTCAAAGTGATTGGCTATACCGATCTTCCGGGCCGTCTGCCGACGCAATCCTCACAGCTTTACGGCAC
TAACCTCGTTAATCTGCTGAAACTGTTGTGCAAAGAGAAAAACGGTAACATCACTGTTGATTTTGATGATGTGGTGATTC
GCGGCGTGACTGTGATCCGTGCGGGCGAAATTACCTGGCCGGCACCGCCGATTCAGGTATCAGCTCAGCCGCAGGTGGCA
CAAAAAGCGGCACCGGAAGTGAAAACTGAGGAAAAATGTGCCTGTTCACCGTGGCGTAAATACGCGTTGATGGCGCTGGC
AATCATCCTTTTCGGCTGGCTGGCAAGCGTTGCGCCGAAAGAGTTTCTTGGGCACTTCACCGTTTTCGCGCTGGCCTGCG
TTGTCGGTTATTACGTGGTGTGGAACGTATCGCACGCGCTGCATACACCGTTGATGTCGGTCACCAACGCGATTTCAGGG
ATTATTGTTGTCGGAGCACTGTTGCAGATTGGCCAGGGCGGCTGGGTTAGCTTCCTTAGTTTTATCGCGGTGCTTATAGC
CAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGCATGCTGAAAATGTTCCGCAAAAATTAA

Upstream 100 bases:

>100_bases
TTGGCGCTAGATCACAGGCATAATTTTCAGTACGTTATAGGGCGTTTGTTACTAATTTATTTTAACGGAGTAACATTTAG
CTCGTACATGAGCGGCTTGT

Downstream 100 bases:

>100_bases
GGGGTAACATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGTCTGGCCGGGC
TTTCGAAACATGAAACGTCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAPDTGKPSSIKPMEGNIMRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNS
VWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAI
VEAAHEFGCFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGS
GDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFT
TENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVA
QKAAPEVKTEEKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISG
IIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_528_residues
MAPDTGKPSSIKPMEGNIMRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNS
VWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAI
VEAAHEFGCFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGS
GDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFT
TENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVA
QKAAPEVKTEEKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISG
IIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_527_residues
APDTGKPSSIKPMEGNIMRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSV
WQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIV
EAAHEFGCFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSG
DGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTT
ENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQ
KAAPEVKTEEKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGI
IVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=50.9398496240602, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=50.9398496240602, Blast_Score=493, Evalue=1e-139,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=98.8235294117647, Blast_Score=1025, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=527, Percent_Identity=50.0948766603416, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 56401; Mature: 56270

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPDTGKPSSIKPMEGNIMRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQL
CCCCCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCC
ASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
CCCCCHHHHHHCHHHCCCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHH
QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAAGK
HHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCC
VPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGS
CCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCC
GDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIV
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
DLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEK
EEECCCCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTEEKCACSPWRK
CCCEEEEEHHHHEECEEEEEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHH
YALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
APDTGKPSSIKPMEGNIMRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQL
CCCCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCC
ASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
CCCCCHHHHHHCHHHCCCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHH
QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAAGK
HHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCC
VPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGS
CCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCC
GDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIV
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
DLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEK
EEECCCCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTEEKCACSPWRK
CCCEEEEEHHHHEECEEEEEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHH
YALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]