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The map label for this gene is yfeR [H]

Identifier: 209397078

GI number: 209397078

Start: 3354426

End: 3355352

Strand: Reverse

Name: yfeR [H]

Synonym: ECH74115_3638

Alternate gene names: 209397078

Gene position: 3355352-3354426 (Counterclockwise)

Preceding gene: 209398574

Following gene: 209396142

Centisome position: 60.22

GC content: 53.94

Gene sequence:

>927_bases
ATGAATTATTCTTTAAAACAATTAAAGGTTTTCGTCACAGTAGCGCAGGAGAAAAGTTTTAGTCGTGCAGGAGAGCGTAT
CGGCCTGAGCCAGTCGGCAGTAAGTCACAGTGTGAAGGAGCTGGAAAATCATACTGGTGTTCGCCTGCTGGACAGAACCA
CGCGTGAAGTGGTGCTTACAGATGCAGGGCAGCAGTTGGCTTTGCGTCTTGAGCGACTGCTGGATGAACTTAACAGCACG
TTGCGCGATACCGGGCGTATGGGGCAACAACTGAGCGGAAAAGTTCGGGTCGCTGCCAGCCAGACCATTTCCGCGCATCT
TATTCCGCAATGCATTGCTGAAAGCCATCGCCGCTATCCAGATATTCAGTTTGTCCTGCACGATCGTCCGCAGCAGTGGG
TGATGGAAAGTATTCGCCAGGGAGATGTCGACTTTGGCATCGTCATCGATCCAGGCCCAGTGGGCGATCTGCAATGTGAA
GTGATTCTTTCCGAGCCTTTCTTTCTGCTTTGCCATCGGGATAGCGCTTTGGCCGCGGAAGATTATGTACCCTGGCAGGC
ATTACAGGGAGCGAAATTGGTGTTGCAGGATTATGCGTCAGGCAGCCGACCGCTGATTGACGCAGCGCTGGCGCGCAATG
GTATTCAGGCGAATATTGTGCAGGAGATTGGACATCCGGCGACGCTGTTCCCGATGGTAGCCGCAGGCATCGGCATCAGT
ATTCTTCCCGCACTGGCGCTACCATTGCCTGAAGGTAGCCCGCTGGTGGTAAAGCGCATTACGCCGGTGGTGGAACGCCA
GTTGATGCTGGTGCGTAGGAAAAACCGTTCACTTTCCACCGCCGCTGAAGCGTTATGGGACGTGGTACGAGACCAGGGCA
ATACGCTAATGGCTGGGCGGGAAGGGGATCCGTTGTATCAGATATAG

Upstream 100 bases:

>100_bases
AAAAGTTTCATAAAGACTCCAGATGATCGATGGTTCTATTGTGCTTCTTCCAGTTTGAGAAATAAAATTGATTTATTGCA
TCCATTCATGAAGAAAACAG

Downstream 100 bases:

>100_bases
ATACCGATATGAGTCGTATTTTCAACAGGATTTAGAAGGGATACATTCAGAGACAAACGCTGCTCTTTCTCCTGCGCCTT
TTCTGCCTGCTGTCTCTGCA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNTLMAGREGDPLYQI

Sequences:

>Translated_308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNTLMAGREGDPLYQI
>Mature_308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNTLMAGREGDPLYQI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=308, Percent_Identity=99.025974025974, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI145693105, Length=316, Percent_Identity=32.2784810126582, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=29.5546558704453, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790399, Length=291, Percent_Identity=27.4914089347079, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=28.0821917808219, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=29.9180327868852, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=27.8688524590164, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=27.6859504132231, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=28.9940828402367, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787128, Length=123, Percent_Identity=34.9593495934959, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081904, Length=179, Percent_Identity=29.608938547486, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786401, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=169, Percent_Identity=27.810650887574, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789506, Length=292, Percent_Identity=23.6301369863014, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787589, Length=122, Percent_Identity=32.7868852459016, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=246, Percent_Identity=22.3577235772358, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=303, Percent_Identity=25.0825082508251, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788296, Length=251, Percent_Identity=26.6932270916335, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=167, Percent_Identity=27.5449101796407, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786713, Length=123, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=266, Percent_Identity=21.8045112781955, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786508, Length=99, Percent_Identity=31.3131313131313, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=125, Percent_Identity=32.8, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=28.8135593220339, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33934; Mature: 33934

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLT
CCCCHHHHEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEE
DAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
DIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCEVILSEPFFLLCHRDSALAAE
CEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHH
DYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
CCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHH
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNTLMAGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECC
EGDPLYQI
CCCCCCCC
>Mature Secondary Structure
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLT
CCCCHHHHEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEE
DAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
DIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCEVILSEPFFLLCHRDSALAAE
CEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHH
DYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
CCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHH
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNTLMAGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECC
EGDPLYQI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]