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The map label for this gene is gltX [H]

Identifier: 209396142

GI number: 209396142

Start: 3351903

End: 3353318

Strand: Reverse

Name: gltX [H]

Synonym: ECH74115_3632

Alternate gene names: 209396142

Gene position: 3353318-3351903 (Counterclockwise)

Preceding gene: 209397078

Following gene: 209400602

Centisome position: 60.18

GC content: 53.04

Gene sequence:

>1416_bases
ATGAAAATCAAAACTCGCTTCGCGCCAAGCCCAACAGGCTATCTGCACGTTGGCGGCGCGCGTACTGCTCTTTACTCCTG
GCTTTTTGCACGTAATCACGGCGGTGAGTTCGTGCTGCGTATTGAAGACACCGATCTTGAGCGTTCCACGCCGGAAGCTA
TCGAAGCCATTATGGATGGCATGAACTGGCTGAGCCTGGAGTGGGATGAAGGTCCGTACTTCCAGACCAAACGTTTTGAT
CGCTACAACGCGGTGATCGATCAGATGCTGGAAGAGGGCACAGCTTATAAATGCTATTGCTCTAAAGAGCGCCTGGAAGC
GCTGCGCGAAGAGCAAATGGCGAAAGGTGAGAAGCCGCGTTATGACGGTCGCTGCCGCCACAGTCATGAGCATCACGCTG
ATGATGAGCCGTGTGTCGTACGTTTTGCTAACCCGCAGGAAGGTTCTGTTGTTTTTGACGATCAGATCCGTGGTCCGATC
GAGTTCAGCAACCAGGAACTGGACGATCTTATTATCCGCCGTACCGATGGTTCCCCAACCTATAACTTCTGTGTGGTTGT
CGATGACTGGGATATGGAAATCACCCACGTTATCCGTGGCGAAGACCATATCAACAACACGCCACGCCAGATCAACATTC
TTAAAGCCCTGAAAGCGCCGGTACCGGTTTACGCGCACGTTTCTATGATCAACGGCGATGACGGCAAAAAACTGTCCAAA
CGTCACGGGGCAGTCAGCGTAATGCAGTATCGTGATGACGGTTATTTGCCAGAAGCACTGCTGAACTATCTGGTGCGTCT
GGGCTGGTCCCACGGCGATCAGGAAATCTTCACTCGTGAAGAGATGATCAAATACTTCACTTTGAATGCCGTCAGCAAAT
CTGCCAGTGCGTTCAACACCGACAAGCTGCTGTGGCTGAACCATCACTACATTAACGCGCTGCCGCCGGAGTATGTTGCT
ACTCACTTACAGTGGCACATTGAGCAGGAAAATATCGATACCCGTAACGGCCCGCAGCTGGCTGATCTGGTGAAACTGCT
GGGCGAACGCTGCAAGACGCTGAAAGAGATGGCACAGAGCTGCCGTTATTTCTACGAAGATTTTGCTGAGTTCGATGCCG
ACGCCGCGAAAAAACATCTGCGTCCGGTAGCGCGTCAGCCGCTGGAAGTGGTTCGTGACAAACTGGCCGCGATTACTGAC
TGGACAGCTGAAAACGTTCATCACGCTATTCAGGCGACGGCGGATGAGCTGGAAGTGGGTATGGGTAAAGTTGGTATGCC
GCTGCGTGTCGCCGTAACCGGTGCGGGGCAGTCTCCGGCGCTGGACGTTACCGTTCACGCGATCGGTAAGACCCGCAGTA
TCGAGCGTATCAACAAAGCGCTGGCTTTTATTGCGGAACGCGAAAATCAGCAGTAA

Upstream 100 bases:

>100_bases
AACTACATTGAGGAATCAGGCGGGAGTGATAGAATATCGCCCACTCAATTTTTCCAGGATTTGCCGGTTGTCGGCATCTT
TCTAAACGTAAGGCCATTTC

Downstream 100 bases:

>100_bases
TTAGCGCATAAAAGATAAACGGCAGGAGAGATACCCCTGCCGTTTTTTATTTATCCGGTGCGCCGTAAAACCCCGTCCTT
CAGGGCGGGGATATAAGGCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALAFIAERENQQ

Sequences:

>Translated_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALAFIAERENQQ
>Mature_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITD
WTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALAFIAERENQQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.9716599190283, Blast_Score=225, Evalue=7e-59,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=99.5753715498938, Blast_Score=985, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=301, Percent_Identity=34.21926910299, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=30.4979253112033, Blast_Score=211, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=32.5301204819277, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=26.9565217391304, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=31.5384615384615, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=30.5498981670061, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53756; Mature: 53756

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQS
CEEEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITDWTAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALAFIAERENQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQS
CEEEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITDWTAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALAFIAERENQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA