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The map label for this gene is mdtK [H]

Identifier: 209397056

GI number: 209397056

Start: 2249463

End: 2250836

Strand: Direct

Name: mdtK [H]

Synonym: ECH74115_2376

Alternate gene names: 209397056

Gene position: 2249463-2250836 (Clockwise)

Preceding gene: 209395995

Following gene: 209399641

Centisome position: 40.37

GC content: 53.35

Gene sequence:

>1374_bases
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGC
GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT
GGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGA
CGTGAGCGCATTGCGCATCAGGTGCGACAGGGCTTCTGGCTGGCGGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTG
GAATGCAGGTTACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGT
TGCTGTGGGGCGCGCCGGGCTATCTTTTCTTCCAGGTTGCCCGTAACCAATGTGAAGGTCTGGCAAAAACCAAGCCGGGT
ATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATTCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGA
GCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAAC
GCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAACCCGATCCTGCGGTTATGAAACGACTGATTCAA
CTCGGTTTGCCGATTGCGCTGGCACTATTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGG
TATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAG
CGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATG
GTGGGTGTCTGTATGGCAACCCTGACGGCCATATTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAA
TCCCGAGGTTGTAACGCTGGCGGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTG
GCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATCACCTTTACGGCTTACTGGGTGCTGGGCTTG
CCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTAT
TGGCCTGACGTCGGCAGCCATTATGATGATGCTGCGTATGCGGTACCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAC
GAGCATCCCGCTAA

Upstream 100 bases:

>100_bases
TCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCTATTCAGGCGGCTTTTTAGTCTCTCATATAAC
TACAAATAAAAGGTGTTCAC

Downstream 100 bases:

>100_bases
TAAAGACAAGGCGCAACCTTCACGGGGTGCGCCTGTGTTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCT
GGCTGTAGTTTCAGTGCGTT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRASR

Sequences:

>Translated_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRASR
>Mature_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRASR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=442, Percent_Identity=21.4932126696833, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI153792564, Length=424, Percent_Identity=20.0471698113208, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38261960, Length=460, Percent_Identity=19.7826086956522, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=99.781181619256, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=459, Percent_Identity=23.7472766884532, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=388, Percent_Identity=20.6185567010309, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320545, Length=439, Percent_Identity=21.4123006833713, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49464; Mature: 49464

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRASR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRASR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA