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The map label for this gene is dadX

Identifier: 209396444

GI number: 209396444

Start: 1617070

End: 1618140

Strand: Direct

Name: dadX

Synonym: ECH74115_1677

Alternate gene names: 209396444

Gene position: 1617070-1618140 (Clockwise)

Preceding gene: 209395760

Following gene: 209400733

Centisome position: 29.02

GC content: 57.52

Gene sequence:

>1071_bases
ATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGCCGCA
CGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCACGGTATTGAGCGTATCTGGAGCGCGCTCGGGGCTACCG
ATGGCTTTGCATTACTTAACCTGGAAGAAGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAA
GGATTTTTCCATGCTCAGGATCTGGAAATGTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAA
AGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCC
AGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCGATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTT
GCCGAGGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGAGTGTCGGCG
TTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCAGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATG
GCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACTGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATT
GGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGTTATACTGCGCGCGATGAGCAGCGAATCGGCAT
TGTTGCCGCAGGATACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCA
TGACGGTGGGGACAGTCTCGATGGATATGCTGGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCAGTT
GAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATGTGCGCGCT
GGCGTTACGCGTCCCGGTTGTGACGGTGTAA

Upstream 100 bases:

>100_bases
AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCG
CACACAGCTAAGGAAACGAG

Downstream 100 bases:

>100_bases
CTTGTTGTAAGTCGGATCGGAGGCAACGTCTTCTGGTTGCAAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTG
TTAATGTGACAGAGCCATTG

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEMYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Sequences:

>Translated_356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEMYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
>Mature_355_residues
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLEG
FFHAQDLEMYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFA
EAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=99.1573033707865, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=346, Percent_Identity=42.7745664739884, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_ECO57 (Q8X4I9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85697
- PIR:   E90839
- RefSeq:   NP_287429.1
- RefSeq:   NP_309712.1
- ProteinModelPortal:   Q8X4I9
- SMR:   Q8X4I9
- EnsemblBacteria:   EBESCT00000027591
- EnsemblBacteria:   EBESCT00000057714
- GeneID:   913184
- GeneID:   960219
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1953
- KEGG:   ecs:ECs1685
- GeneTree:   EBGT00050000009862
- HOGENOM:   HBG712172
- OMA:   WGKQVLA
- ProtClustDB:   PRK03646
- BioCyc:   ECOL83334:ECS1685-MONOMER
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 38859; Mature: 38728

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 35-35 ACT_SITE 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796